Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,295 G→A 5.3% F19F (TTC→TTT cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2950GA 5.3% 421.9 / 8.8 150F19F (TTC→TTTcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (67/75);  new base A (5/3);  total (72/78)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 157 total aligned reads displayed.

TTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCT  >  Exported/1211225‑1211364
                                                                      |                                                                     
ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg                                                                       <  1:4816761/71‑1 (MQ=255)
  ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                      <  1:2205268/70‑1 (MQ=255)
  ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                      <  1:1533115/70‑1 (MQ=255)
        ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa                                                               <  1:2066571/71‑1 (MQ=255)
         cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa                                                               <  1:951577/70‑1 (MQ=9)
          aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                             >  1:3805245/1‑71 (MQ=255)
          aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                             >  1:597120/1‑71 (MQ=255)
              gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt                                                          <  1:4722556/68‑1 (MQ=12)
              ctcTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt                                                         <  1:2084618/69‑1 (MQ=9)
              cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                          <  1:3886370/70‑1 (MQ=255)
              cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGATGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                          >  1:4280542/1‑70 (MQ=255)
              cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                          <  1:4050024/70‑1 (MQ=255)
                cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                       <  1:3836516/71‑1 (MQ=255)
                 tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaaac                                                                   >  1:5419329/1‑56 (MQ=18)
                 tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt                                                          >  1:4297971/1‑67 (MQ=12)
                 tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa                                                      >  1:290164/1‑71 (MQ=255)
                  ggATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc                                                     >  1:1426323/1‑71 (MQ=255)
                   gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa                                                      >  1:4172713/1‑69 (MQ=255)
                   gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt                                                    <  1:523556/71‑1 (MQ=12)
                   gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt                                                    >  1:2843540/1‑71 (MQ=255)
                        cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtta                                               <  1:1167976/69‑2 (MQ=255)
                        cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt                                                <  1:3159219/68‑1 (MQ=12)
                        cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt                                                >  1:4202839/3‑70 (MQ=12)
                        cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa                                                        <  1:5060615/62‑1 (MQ=255)
                         ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                              <  1:303111/71‑1 (MQ=255)
                         ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                              <  1:5539536/71‑1 (MQ=255)
                         ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                              <  1:1613290/71‑1 (MQ=255)
                          gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc                                                     >  1:3861295/1‑63 (MQ=12)
                          gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc                                                     >  1:2598203/1‑63 (MQ=12)
                            gaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                            >  1:5097356/1‑70 (MQ=255)
                             aaGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                          <  1:3736081/55‑1 (MQ=255)
                              aGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCAc                                         <  1:4487975/71‑1 (MQ=255)
                                tgtAAGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccg                                                                 <  1:1828006/45‑2 (MQ=2)
                                 gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg                                                                       >  1:1775226/1‑38 (MQ=18)
                                 gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg                                                                       >  1:3088576/1‑38 (MQ=18)
                                 gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg                                                                       >  1:1348652/1‑38 (MQ=18)
                                 gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg                                                                       <  1:1027758/38‑1 (MQ=255)
                                  tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaa                                                                     <  1:5693580/39‑1 (MQ=17)
                                  tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa                                     >  1:2964801/1‑71 (MQ=255)
                                  tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa                                     >  1:702603/1‑71 (MQ=255)
                                  tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa                                                        >  1:3796598/1‑52 (MQ=255)
                                  tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt                                                 >  1:2812273/1‑59 (MQ=255)
                                  tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                      <  1:2181620/70‑1 (MQ=255)
                                  tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta                                     >  1:4551257/1‑69 (MQ=255)
                                   aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                             >  1:1208086/1‑46 (MQ=255)
                                   aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                             >  1:3856104/1‑46 (MQ=255)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg                                                                       >  1:4512650/1‑35 (MQ=12)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa                                                                      >  1:2849694/1‑36 (MQ=12)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa                                             >  1:3629146/1‑61 (MQ=9)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc                                            >  1:2622983/1‑62 (MQ=9)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc                                            >  1:2731558/1‑62 (MQ=9)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg                                   >  1:4127071/1‑70 (MQ=12)
                                    cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg                                   >  1:192844/1‑70 (MQ=12)
                                      aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga                                                                >  1:5206818/1‑38 (MQ=9)
                                      aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga                                                                >  1:1525315/1‑38 (MQ=9)
                                       acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa                                <  1:3971008/71‑1 (MQ=255)
                                        cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc                                         <  1:3855277/61‑1 (MQ=255)
                                          cgtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCt                             >  1:3944241/3‑71 (MQ=12)
                                          caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt                             <  1:4781149/71‑1 (MQ=255)
                                           aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                            <  1:4970200/71‑1 (MQ=255)
                                            tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                       >  1:1835602/1‑43 (MQ=255)
                                            tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg                                               <  1:5642545/51‑1 (MQ=255)
                                            tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                      <  1:4881688/60‑1 (MQ=255)
                                            tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                      <  1:5158342/60‑1 (MQ=255)
                                              tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg                                                       <  1:1025835/41‑3 (MQ=12)
                                              tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg                                                        <  1:4885998/40‑2 (MQ=12)
                                              tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg                                 >  1:5654221/1‑63 (MQ=255)
                                                tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                          <  1:2164328/68‑1 (MQ=255)
                                                tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                          <  1:3678572/68‑1 (MQ=255)
                                                tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                          <  1:3454643/68‑1 (MQ=255)
                                                 gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                      >  1:3595011/1‑71 (MQ=255)
                                                  ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtt                     <  1:5041135/71‑1 (MQ=255)
                                                   tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                          <  1:1711364/65‑1 (MQ=255)
                                                   tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                          <  1:402581/65‑1 (MQ=255)
                                                     ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc                  >  1:1797797/1‑71 (MQ=255)
                                                       aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa                <  1:5384946/71‑1 (MQ=255)
                                                        tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                      <  1:3575/64‑1 (MQ=255)
                                                        tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt               <  1:1346808/71‑1 (MQ=255)
                                                        tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt               >  1:5457350/1‑71 (MQ=255)
                                                          aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                    >  1:4610099/1‑64 (MQ=255)
                                                          aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt              >  1:3981886/1‑70 (MQ=255)
                                                            ccGTAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc                                    >  1:3683862/1‑46 (MQ=12)
                                                            ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc                                    >  1:2053871/1‑46 (MQ=16)
                                                            ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa            <  1:4366361/70‑4 (MQ=255)
                                                            ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa            <  1:3496766/70‑4 (MQ=255)
                                                            ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa            <  1:3476175/70‑4 (MQ=255)
                                                            ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca             <  1:3627065/69‑3 (MQ=255)
                                                              ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa                <  1:2943115/64‑1 (MQ=255)
                                                              ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa         <  1:3067404/71‑1 (MQ=255)
                                                                aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt              >  1:3768443/1‑64 (MQ=255)
                                                                aGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtta                    >  1:810974/1‑57 (MQ=16)
                                                                 gTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTAcc                              >  1:3366984/1‑47 (MQ=9)
                                                                  tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                            <  1:4339395/48‑1 (MQ=255)
                                                                   aaTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt                           <  1:3264398/48‑1 (MQ=255)
                                                                    aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                         >  1:4980506/1‑49 (MQ=255)
                                                                    aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc   <  1:2504374/71‑1 (MQ=255)
                                                                    aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc   <  1:3240707/71‑1 (MQ=255)
                                                                    aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc   >  1:3116939/1‑71 (MQ=255)
                                                                    aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc   >  1:3092134/1‑71 (MQ=255)
                                                                     tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt               <  1:4771719/58‑1 (MQ=255)
                                                                      |                                                                     
TTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCT  >  Exported/1211225‑1211364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: