| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,259,295 | G→A | 6.8% | D543D (GAC→GAT) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,259,295 | 0 | G | A | 6.8% | 261.0 / 6.0 | 88 | D543D (GAC→GAT) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (47/35); new base A (2/4); total (49/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCT > Exported/1259228‑1259363 | cATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTc > 1:1751840/1‑70 (MQ=255) aTCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCaa < 1:4661793/71‑1 (MQ=255) tACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCTGCAAGATCGGCAAACACGTCaaa < 1:5420613/67‑1 (MQ=255) tACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCg > 1:768516/1‑71 (MQ=255) ttCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa > 1:4333271/1‑70 (MQ=255) ttCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa > 1:2577817/1‑70 (MQ=255) tCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTAc > 1:3456550/1‑70 (MQ=255) cGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:1015638/66‑1 (MQ=255) ggTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTAcgcg < 1:2240435/71‑1 (MQ=255) gTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:1451785/64‑1 (MQ=255) taGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCaaaa > 1:3051159/1‑57 (MQ=255) taGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCaaaa > 1:4322098/1‑57 (MQ=255) gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa < 1:1482188/70‑1 (MQ=255) gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa > 1:1489408/1‑70 (MQ=255) gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATAGGCAAACACGTCAAAATCGTTACGCGCTTCaa < 1:1294401/71‑1 (MQ=255) aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGt < 1:3541163/49‑1 (MQ=255) aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:3570043/58‑1 (MQ=255) aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:5377354/1‑71 (MQ=255) aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:2502103/1‑71 (MQ=255) aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:5384102/1‑71 (MQ=255) ctctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt > 1:5305766/1‑71 (MQ=255) tctTTTCCGCCAGGTTTGAGTAATTCTGCAAGATCGGCAAACACGTCAAAATCGt < 1:2733187/55‑1 (MQ=255) tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:2201226/55‑1 (MQ=255) tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:1802551/55‑1 (MQ=255) ctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTg > 1:3528852/1‑70 (MQ=255) ctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGc > 1:226675/1‑71 (MQ=255) ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTg < 1:1766721/69‑1 (MQ=255) ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg > 1:4219728/1‑71 (MQ=255) ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg > 1:3646290/1‑71 (MQ=255) ttttCCGCCAGGTTTGAGTAAATCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg > 1:134994/1‑71 (MQ=255) tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt < 1:391081/52‑1 (MQ=255) ttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg > 1:956112/1‑69 (MQ=255) tCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAAt > 1:1460579/1‑47 (MQ=255) tCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa > 1:4531876/1‑52 (MQ=255) tCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa > 1:1767400/1‑52 (MQ=255) cGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa < 1:4013688/61‑1 (MQ=255) gCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCaa < 1:3140806/71‑1 (MQ=255) ccAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt < 1:5290847/61‑1 (MQ=255) aGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAg > 1:4979884/1‑71 (MQ=255) ggTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGc > 1:4241530/1‑71 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa < 1:5367134/53‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa < 1:5406231/53‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:3357967/71‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:3677550/71‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:861176/71‑1 (MQ=255) ttGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:4569347/1‑53 (MQ=255) gTAATTCCGCAAGATCGGCAAACACGTCAAAATCGtt > 1:4067248/1‑37 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa < 1:1722986/46‑1 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc > 1:2259436/1‑64 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt < 1:5173178/69‑1 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt < 1:618322/69‑1 (MQ=255) aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACTCGCTTCAAAtt < 1:5272599/49‑1 (MQ=255) aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt < 1:3670806/49‑1 (MQ=255) aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt < 1:5293583/49‑1 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:1044371/1‑46 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:4725685/1‑46 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:413265/1‑46 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:5453428/1‑46 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:1617232/1‑46 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc > 1:4216608/1‑71 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc > 1:946461/1‑71 (MQ=255) ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:4448310/61‑1 (MQ=255) ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:2233876/61‑1 (MQ=255) tCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg > 1:2372971/1‑71 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:970065/1‑43 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:2127068/1‑43 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:2940991/1‑43 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa > 1:3546744/1‑43 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt > 1:1757209/1‑64 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg > 1:1358318/1‑70 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg > 1:4421697/1‑70 (MQ=255) cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac > 1:5262998/1‑70 (MQ=255) cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac > 1:5671875/1‑70 (MQ=255) aaGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa > 1:3155944/1‑71 (MQ=255) aactCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcac > 1:4615051/4‑68 (MQ=255) gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGc > 1:1478041/1‑41 (MQ=255) gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata > 1:2673821/1‑71 (MQ=255) ctCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacc > 1:5631644/2‑66 (MQ=255) tCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAatat > 1:4082233/1‑70 (MQ=255) ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:1029714/49‑1 (MQ=255) gCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTg < 1:4203601/71‑1 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacca < 1:4822424/62‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:3972096/71‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:4185684/71‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:2588035/71‑3 (MQ=255) aaaCACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGt < 1:5160103/71‑1 (MQ=255) acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa < 1:2597644/60‑1 (MQ=255) acGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt < 1:3570864/71‑1 (MQ=255) | CATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCT > Exported/1259228‑1259363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |