Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,259,384 A→G 17.2% L514L (TTG→CTG) ‡ torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,259,3840AG17.2% 401.4 / 68.8 180L514L (TTG→CTG) ‡torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (62/87);  new base G (13/18);  total (75/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 186 total aligned reads displayed.

AAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAG  >  Exported/1259316‑1259451
                                                                    |                                                                   
aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                                                                   <  1:3778107/71‑1 (MQ=255)
  aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                                                  <  1:5453119/70‑1 (MQ=255)
   ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt                                                                >  1:4026285/1‑71 (MQ=255)
      cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc                                                             >  1:250120/1‑71 (MQ=255)
        gAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                                                                 <  1:2915445/65‑1 (MQ=255)
         aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt                                                          >  1:4602458/1‑71 (MQ=255)
           cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                         >  1:3774467/1‑70 (MQ=255)
           cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                         >  1:1774422/1‑70 (MQ=255)
             aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                                                               >  1:5518979/1‑62 (MQ=255)
             aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                                                               >  1:4889175/1‑62 (MQ=255)
             aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                                                      >  1:4710690/1‑71 (MQ=255)
             aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                                                      >  1:3573577/1‑71 (MQ=255)
              cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                     >  1:5322952/1‑71 (MQ=255)
               aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                     >  1:4465805/1‑70 (MQ=255)
                gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGa                                                   <  1:898039/71‑1 (MQ=255)
                gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGa                                                   >  1:2465211/1‑71 (MQ=255)
                 cttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGag                                                  >  1:4764540/3‑70 (MQ=255)
                  ttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                               <  1:1105413/56‑1 (MQ=255)
                  ttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                               <  1:2268699/56‑1 (MQ=255)
                  cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                                 <  1:2590335/71‑1 (MQ=255)
                   tGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGagg                                                 >  1:299908/1‑68 (MQ=255)
                   tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                >  1:1590814/1‑71 (MQ=255)
                   tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                >  1:2879637/1‑71 (MQ=255)
                    gCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGagg                                                 >  1:4307876/1‑67 (MQ=255)
                    gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                    <  1:5129294/66‑1 (MQ=255)
                    gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                               >  1:5367628/1‑71 (MQ=255)
                    gCTTCATCGGCACAATATGCTGGTTGCTGAAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                               >  1:3960892/1‑71 (MQ=255)
                     cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                              <  1:3553840/71‑1 (MQ=255)
                      ttCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                               >  1:3026116/1‑53 (MQ=255)
                      ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCATAc                                                     <  1:936273/63‑1 (MQ=255)
                      ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                     <  1:1552641/63‑1 (MQ=255)
                      ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                     <  1:5655367/63‑1 (MQ=255)
                       tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                             >  1:3032629/1‑70 (MQ=255)
                       tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                             >  1:4743670/1‑70 (MQ=255)
                        cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtta                                                              >  1:1894571/1‑51 (MQ=255)
                        cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtta                                                              >  1:4800512/1‑51 (MQ=255)
                        cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                            <  1:1185271/70‑2 (MQ=255)
                         aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                          <  1:1655858/71‑1 (MQ=255)
                         aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                          <  1:820891/71‑1 (MQ=255)
                         aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                          <  1:2179754/71‑1 (MQ=255)
                         aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                          <  1:3427176/71‑1 (MQ=255)
                          tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                                        <  1:5094596/56‑1 (MQ=255)
                          tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                                        <  1:4401480/56‑1 (MQ=255)
                          tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                <  1:2926890/64‑1 (MQ=255)
                            ggCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTacg                                                            >  1:1250668/1‑50 (MQ=255)
                                caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                    <  1:490495/70‑1 (MQ=255)
                                 aaTATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATa                                  <  1:5124843/71‑1 (MQ=255)
                                   tatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTTCGCTCAAACGATGTGGTGATCGGTAATACg                                <  1:3512811/71‑1 (MQ=255)
                                   tatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                    <  1:318329/51‑1 (MQ=255)
                                    atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                              <  1:1144911/56‑3 (MQ=255)
                                    atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                              <  1:2124817/56‑3 (MQ=255)
                                     tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                        <  1:3453030/61‑1 (MQ=255)
                                     tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                        <  1:1314701/61‑1 (MQ=255)
                                      gCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTc                                                         <  1:1286203/43‑1 (MQ=12)
                                      gCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                            >  1:1265162/1‑55 (MQ=255)
                                       cTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt                                      >  1:1988109/1‑61 (MQ=255)
                                         ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                               <  1:2387688/50‑1 (MQ=255)
                                         ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                              >  1:1739802/1‑51 (MQ=255)
                                           ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                    <  1:2762776/59‑1 (MQ=255)
                                           ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGc                        <  1:686288/71‑1 (MQ=255)
                                            tGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCg                       >  1:4543770/1‑71 (MQ=255)
                                              cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                            <  1:5223313/64‑1 (MQ=255)
                                              cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                            <  1:1794790/64‑1 (MQ=255)
                                              cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                            <  1:2739635/64‑1 (MQ=255)
                                              cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                     <  1:3827125/71‑1 (MQ=255)
                                               tGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGat                              <  1:4050599/61‑1 (MQ=255)
                                                 tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                   <  1:3663348/54‑1 (MQ=255)
                                                 tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                   <  1:862157/54‑1 (MQ=255)
                                                 tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtgt                    <  1:3881979/69‑1 (MQ=255)
                                                  aaTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTg                 >  1:1209252/1‑71 (MQ=255)
                                                   aTCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGtttt                 <  1:2177162/70‑2 (MQ=255)
                                                    tCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTgg                >  1:2713331/1‑70 (MQ=255)
                                                    tCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgc               <  1:4492868/71‑1 (MQ=255)
                                                     cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                               >  1:5627515/1‑38 (MQ=255)
                                                     cACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                            <  1:5570248/57‑1 (MQ=255)
                                                         ggTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATa                                  >  1:2897523/1‑47 (MQ=255)
                                                          gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                <  1:3171506/48‑1 (MQ=255)
                                                          gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                <  1:4284283/48‑1 (MQ=255)
                                                          gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                <  1:4564207/48‑1 (MQ=255)
                                                          gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc         >  1:3286922/1‑71 (MQ=255)
                                                          gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc         <  1:5486043/71‑1 (MQ=255)
                                                            cATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                        >  1:764864/1‑38 (MQ=255)
                                                             aTCGTCAATTCATTGCGCTCAAACGATGTGGTGATCGGt                                      >  1:5305472/1‑39 (MQ=255)
                                                             aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                     >  1:4650951/1‑40 (MQ=255)
                                                             aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                     >  1:4114240/1‑40 (MQ=255)
                                                             aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                     >  1:4120518/1‑40 (MQ=255)
                                                             aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCAGCGTGTTTGGCTGCTGCgg       <  1:4417518/70‑1 (MQ=255)
                                                              tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:2339613/71‑1 (MQ=255)
                                                              tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:5424269/71‑1 (MQ=255)
                                                              tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:3350016/71‑1 (MQ=255)
                                                               cGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     >  1:1160132/1‑70 (MQ=255)
                                                                gTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGtt                   >  1:2228763/1‑55 (MQ=255)
                                                                gTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCg        >  1:4972986/1‑66 (MQ=255)
                                                                gTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCg        >  1:5668380/1‑66 (MQ=255)
                                                                 tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:2079134/68‑1 (MQ=255)
                                                                 tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:5381198/68‑1 (MQ=255)
                                                                 tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc     <  1:420713/68‑1 (MQ=255)
                                                                   aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc            <  1:1397707/59‑1 (MQ=255)
                                                                   aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg  >  1:5511488/1‑69 (MQ=255)
                                                                   aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg  >  1:1000552/1‑69 (MQ=255)
                                                                    |                                                                   
AAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAG  >  Exported/1259316‑1259451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: