Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,259,793 C→T 27.4% Q377Q (CAG→CAA torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,259,7930CT27.4% 75.4 / 29.9 51Q377Q (CAG→CAAtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (17/20);  new base T (10/4);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC  >  Exported/1259723‑1259863
                                                                      |                                                                      
cAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATc                                                                        >  1:2102408/1‑71 (MQ=255)
 aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCt                                                                       >  1:109426/1‑71 (MQ=255)
       cgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCt                                                                  <  1:1088197/70‑1 (MQ=255)
       cgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTa                                                                 <  1:1946550/71‑1 (MQ=255)
         tggggTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCaa                                                                >  1:3184431/3‑70 (MQ=255)
         tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTa                                                                 <  1:742430/69‑1 (MQ=255)
             gTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACAttg                                                           >  1:1004409/1‑69 (MQ=255)
              ttaccaccaTTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATtgc                                                          >  1:2755281/4‑71 (MQ=255)
              ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATcgc                                                          >  1:4433378/1‑71 (MQ=255)
               taccaccaTTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATtgc                                                          >  1:239651/3‑70 (MQ=255)
                          gAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCAc                                              <  1:3032831/71‑1 (MQ=255)
                          gAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCAc                                              <  1:2844667/71‑1 (MQ=255)
                           aGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCAc                                              >  1:4600250/1‑70 (MQ=255)
                             aaaTGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGc                                                   <  1:3618007/62‑1 (MQ=255)
                             aaaTGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGAcga                                            <  1:419678/69‑3 (MQ=255)
                                  gtAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATcc                                      <  1:4525914/70‑1 (MQ=255)
                                  gtAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATcc                                      <  1:138006/70‑1 (MQ=255)
                                   tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                       >  1:3898621/1‑69 (MQ=255)
                                      gagaAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGc                                          <  1:1149535/63‑1 (MQ=255)
                                      gagaAACCAAAACCGCCGCACGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGc                                          <  1:248870/63‑1 (MQ=255)
                                        gaAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACAt                                                             >  1:3003638/1‑42 (MQ=255)
                                        gaAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                >  1:2201941/1‑71 (MQ=255)
                                         agaCCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCa                                     >  1:402649/3‑65 (MQ=255)
                                          gaCCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGtttt                              >  1:4128930/2‑70 (MQ=255)
                                          aaCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGc                                                   <  1:3615561/50‑1 (MQ=255)
                                          aaCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCa                                         <  1:908069/60‑1 (MQ=255)
                                          aaCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                              <  1:2122035/71‑1 (MQ=255)
                                           aCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                              >  1:2733240/1‑70 (MQ=255)
                                            ccAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCa                                            >  1:2902326/1‑55 (MQ=255)
                                             cAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATtgct                                                         >  1:3988192/1‑40 (MQ=12)
                                             cAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGtttt                              >  1:3137196/1‑67 (MQ=255)
                                             cAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                      <  1:1660741/60‑1 (MQ=255)
                                             cAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                   <  1:3178490/63‑1 (MQ=255)
                                                aaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCa                        >  1:5384322/1‑71 (MQ=255)
                                                aaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCa                        >  1:2015270/1‑71 (MQ=255)
                                                   cgccgcCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt                           <  1:317868/65‑1 (MQ=255)
                                                    gccgccGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCa                     >  1:2928180/1‑70 (MQ=255)
                                                    gccgccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                     >  1:3376499/1‑70 (MQ=255)
                                                      cgccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                            <  1:5535675/61‑1 (MQ=255)
                                                       gccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                               <  1:2246028/57‑1 (MQ=255)
                                                       gccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                 <  1:4789950/71‑1 (MQ=255)
                                                        ccgggTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCa                        >  1:3004314/4‑63 (MQ=255)
                                                        cccGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTg                >  1:2805669/1‑71 (MQ=255)
                                                          cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGGATCCAGTGTTTCTGTTCACCATATTGCt              <  1:3908737/71‑1 (MQ=255)
                                                          cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                    >  1:1119392/1‑49 (MQ=255)
                                                             tgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc           >  1:2667483/1‑71 (MQ=255)
                                                               tACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                            <  1:1224449/52‑1 (MQ=255)
                                                               tACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                            <  1:3432571/52‑1 (MQ=255)
                                                                 ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtgt                                 >  1:1547495/1‑45 (MQ=255)
                                                                 ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtgt                                 >  1:2318236/1‑45 (MQ=255)
                                                                 ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtgt                                 >  1:2839865/1‑45 (MQ=255)
                                                                     tCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt              <  1:148507/60‑1 (MQ=255)
                                                                      cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc  >  1:4127653/1‑71 (MQ=255)
                                                                      cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc  >  1:3686733/1‑71 (MQ=255)
                                                                      cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc  >  1:761480/1‑71 (MQ=255)
                                                                      |                                                                      
CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC  >  Exported/1259723‑1259863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: