Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,260,329 A→T 14.8% S199T (TCC→ACC) ‡ torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,260,3290AT14.8% 206.6 / 26.3 81S199T (TCC→ACC) ‡torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (37/32);  new base T (8/4);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

AAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTT  >  Exported/1260262‑1260396
                                                                   |                                                                   
aaGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag                                                                    >  1:1400898/1‑69 (MQ=255)
 aGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCATAATCAGCGGCCAGGAGGTc                                                                 >  1:3443078/1‑71 (MQ=255)
 aGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTc                                                                 >  1:711866/1‑71 (MQ=255)
 aGGTATTCAGCGGGTTCATTCCCCACAGCACCAAAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGt                                                                  <  1:2277717/70‑1 (MQ=255)
    tATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc                                                              <  1:4918965/71‑1 (MQ=255)
     aTTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc                                                              >  1:4336443/1‑70 (MQ=255)
      ttCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgctg                                                            <  1:5566174/71‑1 (MQ=255)
        cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt                                                           >  1:4141498/1‑70 (MQ=255)
          gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                                        <  1:5631559/71‑1 (MQ=255)
           cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGt                                                                  >  1:3354627/1‑60 (MQ=255)
           cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                                        >  1:1336383/1‑70 (MQ=255)
             ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag                                                                    >  1:3723437/1‑56 (MQ=255)
             ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag                                                                    >  1:3823476/1‑56 (MQ=255)
             ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag                                                                    >  1:1223074/1‑56 (MQ=255)
             ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATac                                                     <  1:4160766/71‑1 (MQ=255)
             ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATAAGCGGCCaggag                                                                    >  1:3482302/1‑56 (MQ=255)
              gTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca                                                    <  1:352442/71‑1 (MQ=255)
                tCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACAcc                                                  >  1:1785152/1‑71 (MQ=255)
                  aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                                        <  1:3573935/63‑1 (MQ=255)
                  aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca                                                    >  1:5071132/1‑67 (MQ=255)
                  aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca                                                    >  1:2900489/1‑67 (MQ=255)
                  aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa                                                      >  1:4038360/1‑65 (MQ=255)
                    tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                              >  1:4819010/1‑71 (MQ=255)
                    tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                              >  1:4327800/1‑71 (MQ=255)
                       ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                                           <  1:4607476/71‑1 (MQ=255)
                       ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                                           <  1:5527836/71‑1 (MQ=255)
                        cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaac                                                   <  1:1399712/62‑1 (MQ=255)
                        cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttcac                                              >  1:2175164/1‑65 (MQ=255)
                          caGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaact                                                  >  1:4406122/1‑60 (MQ=255)
                           aGCACCACGACATCGCTATGTTCCAGCACCAGCGGTCAACTGGTCTGCTGTTGATaaa                                                    >  1:4046215/1‑56 (MQ=255)
                           aGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa                                                    >  1:5234272/1‑56 (MQ=255)
                           aGCACCAAGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa                                                    >  1:4937699/1‑56 (MQ=255)
                            gCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                       >  1:4129664/1‑70 (MQ=255)
                             caccacGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa                                                    >  1:3883834/1‑54 (MQ=255)
                               ccacAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt                                                           <  1:5568185/47‑1 (MQ=255)
                               ccacAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt                                                           <  1:1189137/47‑1 (MQ=255)
                                cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa                                            <  1:3424540/61‑1 (MQ=255)
                                cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca                                  <  1:657866/71‑1 (MQ=255)
                                 acaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac                                 <  1:1199900/71‑1 (MQ=255)
                                  caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACctc                                                <  1:2356329/55‑1 (MQ=255)
                                  caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                >  1:2621935/1‑71 (MQ=255)
                                   aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt                               >  1:745218/1‑71 (MQ=255)
                                    aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                <  1:5497130/69‑1 (MQ=255)
                                    aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg                              >  1:1607392/1‑71 (MQ=255)
                                    aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg                              <  1:1657138/71‑1 (MQ=255)
                                      cTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCg                            <  1:1819726/71‑1 (MQ=255)
                                        ggCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg                           >  1:5348665/1‑70 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                       <  1:3638016/53‑1 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                       <  1:380373/53‑1 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                >  1:2118921/1‑60 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                >  1:2125503/1‑60 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa                         <  1:4982701/67‑1 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa                         <  1:3013841/67‑1 (MQ=255)
                                             ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa                      >  1:2053805/1‑70 (MQ=255)
                                             tgttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGa                      <  1:3837518/68‑1 (MQ=255)
                                              tttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa                                                      >  1:4256130/1‑37 (MQ=255)
                                              tttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa                                                      >  1:1862993/1‑37 (MQ=255)
                                               ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt                     >  1:3448164/1‑69 (MQ=255)
                                               ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                   <  1:5635217/71‑2 (MQ=255)
                                               ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                   >  1:4888777/1‑70 (MQ=255)
                                               ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                   <  1:3771724/71‑2 (MQ=255)
                                               ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                >  1:5386442/1‑58 (MQ=255)
                                               ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac                                 >  1:2792368/1‑57 (MQ=255)
                                               ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                       >  1:5605029/1‑67 (MQ=255)
                                               ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                       >  1:3667326/1‑67 (MQ=255)
                                               ttCCAGAATCAGCGGCCAGGAGGTCTGCAGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                       >  1:1734160/1‑67 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc                    <  1:4622432/68‑1 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc                    <  1:1905987/68‑1 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAcc                 >  1:2463294/1‑71 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                  <  1:4802583/70‑1 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                  <  1:3754421/70‑1 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                  <  1:65811/70‑1 (MQ=255)
                                                 ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                  >  1:1843578/1‑70 (MQ=255)
                                                   aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGa                                          <  1:2130681/44‑1 (MQ=255)
                                                    gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa                                            <  1:4680426/41‑1 (MQ=255)
                                                     aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg             >  1:2739886/1‑71 (MQ=255)
                                                     aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg             >  1:636680/1‑71 (MQ=255)
                                                       ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc                  >  1:4428977/2‑64 (MQ=255)
                                                       ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc                  >  1:2469112/2‑64 (MQ=255)
                                                       ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgcg             >  1:2004066/2‑67 (MQ=255)
                                                           cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt              <  1:2812304/64‑1 (MQ=255)
                                                           cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt              <  1:1127941/64‑1 (MQ=255)
                                                           cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGccc       <  1:3558249/71‑1 (MQ=255)
                                                             gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg     >  1:2095323/1‑71 (MQ=255)
                                                             gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg     >  1:3156104/1‑71 (MQ=255)
                                                               cAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCAGCGCAGCACCgg    >  1:1902837/1‑70 (MQ=255)
                                                                aggaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGtt  >  1:2762715/1‑71 (MQ=255)
                                                                  gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                  >  1:2985231/1‑53 (MQ=255)
                                                                   |                                                                   
AAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTT  >  Exported/1260262‑1260396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: