Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,260,352 A→C 7.7% V191G (GTA→GGA) ‡ torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,260,3520AC 7.7% 195.7 / 9.8 78V191G (GTA→GGA) ‡torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (36/36);  new base C (3/3);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

TCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAA  >  Exported/1260282‑1260420
                                                                      |                                                                    
tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                                                      >  1:4327800/1‑71 (MQ=255)
tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                                                      >  1:4819010/1‑71 (MQ=255)
   ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                                                                   <  1:5527836/71‑1 (MQ=255)
   ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                                                                   <  1:4607476/71‑1 (MQ=255)
        gCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                                               >  1:4129664/1‑70 (MQ=255)
            cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa                                                                    <  1:3424540/61‑1 (MQ=255)
            cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca                                                          <  1:657866/71‑1 (MQ=255)
             acaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac                                                         <  1:1199900/71‑1 (MQ=255)
              caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                                        >  1:2621935/1‑71 (MQ=255)
               aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt                                                       >  1:745218/1‑71 (MQ=255)
                aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                                        <  1:5497130/69‑1 (MQ=255)
                aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg                                                      <  1:1657138/71‑1 (MQ=255)
                aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg                                                      >  1:1607392/1‑71 (MQ=255)
                  cTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCg                                                    <  1:1819726/71‑1 (MQ=255)
                    ggCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg                                                   >  1:5348665/1‑70 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                                               <  1:380373/53‑1 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc                                                               <  1:3638016/53‑1 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                                        >  1:2125503/1‑60 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                                        >  1:2118921/1‑60 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa                                                 <  1:3013841/67‑1 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa                                                 <  1:4982701/67‑1 (MQ=255)
                         ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa                                              >  1:2053805/1‑70 (MQ=255)
                         tgttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGa                                              <  1:3837518/68‑1 (MQ=255)
                           ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt                                             >  1:3448164/1‑69 (MQ=255)
                           ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                                           >  1:4888777/1‑70 (MQ=255)
                           ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                                           <  1:3771724/71‑2 (MQ=255)
                           ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg                                           <  1:5635217/71‑2 (MQ=255)
                           ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                                                        >  1:5386442/1‑58 (MQ=255)
                           ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac                                                         >  1:2792368/1‑57 (MQ=255)
                           ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                                               >  1:5605029/1‑67 (MQ=255)
                           ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                                               >  1:3667326/1‑67 (MQ=255)
                           ttCCAGAATCAGCGGCCAGGAGGTCTGCAGTTCATACACCTCTACAGAACCGACCACATGCGGCATg                                               >  1:1734160/1‑67 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc                                            <  1:4622432/68‑1 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc                                            <  1:1905987/68‑1 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAcc                                         >  1:2463294/1‑71 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                                          <  1:65811/70‑1 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                                          >  1:1843578/1‑70 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                                          <  1:4802583/70‑1 (MQ=255)
                             ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                                          <  1:3754421/70‑1 (MQ=255)
                               aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGa                                                                  <  1:2130681/44‑1 (MQ=255)
                                gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa                                                                    <  1:4680426/41‑1 (MQ=255)
                                 aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg                                     >  1:636680/1‑71 (MQ=255)
                                 aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg                                     >  1:2739886/1‑71 (MQ=255)
                                   ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc                                          >  1:2469112/2‑64 (MQ=255)
                                   ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc                                          >  1:4428977/2‑64 (MQ=255)
                                   ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgcg                                     >  1:2004066/2‑67 (MQ=255)
                                       cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt                                      <  1:2812304/64‑1 (MQ=255)
                                       cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt                                      <  1:1127941/64‑1 (MQ=255)
                                       cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGccc                               <  1:3558249/71‑1 (MQ=255)
                                         gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg                             >  1:2095323/1‑71 (MQ=255)
                                         gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg                             >  1:3156104/1‑71 (MQ=255)
                                           cAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCAGCGCAGCACCgg                            >  1:1902837/1‑70 (MQ=255)
                                            aggaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGtt                          >  1:2762715/1‑71 (MQ=255)
                                              gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc                                          >  1:2985231/1‑53 (MQ=255)
                                               tggTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt                                             >  1:4584112/2‑49 (MQ=255)
                                                ggTTTGCTGTTCATACACTTCCATCGAACCGACTAAGCGCGGCAGGATCACCTGCGCAGCACAGGTAGag                       >  1:866146/1‑69 (MQ=255)
                                                  tCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa                    <  1:3517141/71‑1 (MQ=255)
                                                   ctgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataat                   >  1:2371063/1‑71 (MQ=255)
                                                   ctgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataat                   >  1:5492888/1‑71 (MQ=255)
                                                    tgctgTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGCGCAGCACCGGTAGagtaat                   <  1:5009371/70‑1 (MQ=255)
                                                    tgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc                                            <  1:2666496/45‑1 (MQ=255)
                                                    tgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATc                  >  1:3645477/1‑71 (MQ=255)
                                                       tgTTCATACACCTCTACAGAACCGACCACATGCgg                                                   <  1:702544/35‑1 (MQ=255)
                                                       tgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgcc               >  1:181128/1‑71 (MQ=255)
                                                         ttCATACACCTCTACAGCACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaat                      <  1:4559352/62‑1 (MQ=255)
                                                         ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaat                      <  1:3231834/62‑1 (MQ=255)
                                                         ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgccgc             <  1:1894208/71‑1 (MQ=255)
                                                           cATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa                    >  1:4765835/1‑62 (MQ=255)
                                                           cATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa                    >  1:440420/1‑62 (MQ=255)
                                                             tACACCTCTACAGAACCGACCACATGCGGCATGATCa                                           <  1:1579972/37‑1 (MQ=255)
                                                             tACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgctgc                                 <  1:4105586/47‑1 (MQ=255)
                                                               cacCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGccc                               >  1:1769775/1‑47 (MQ=255)
                                                                 ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg     <  1:5546190/71‑1 (MQ=255)
                                                                 ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg     <  1:2810837/71‑1 (MQ=255)
                                                                  ctctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg     >  1:2166735/1‑70 (MQ=255)
                                                                   tctACAGAACCGACCACATGCGGCATGATCACCtg                                       <  1:5660438/35‑1 (MQ=255)
                                                                    ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg     <  1:3560212/68‑1 (MQ=255)
                                                                    ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg     <  1:949837/68‑1 (MQ=255)
                                                                    ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGaa  >  1:4810591/1‑71 (MQ=255)
                                                                     tACAGAACCGACCACATGCGGCATGATCACCtgtg                                     >  1:167511/1‑35 (MQ=255)
                                                                      |                                                                    
TCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAA  >  Exported/1260282‑1260420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: