| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 223,030 | T→G | 6.6% | intergenic (+85/‑95) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 223,030 | 0 | T | G | 6.6% | 353.7 / 13.9 | 138 | intergenic (+85/‑95) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base T (62/66); new base G (6/3); total (69/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 141 total aligned reads displayed.
TAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCTTA > Exported/222963‑223100 | aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:75157/67‑1 (MQ=11) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:3666333/1‑71 (MQ=255) gTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaa > 1:1943052/1‑71 (MQ=14) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATg > 1:1076548/1‑63 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGc > 1:2237443/1‑64 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa > 1:2742367/1‑71 (MQ=255) ttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaact > 1:187324/1‑69 (MQ=11) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:5551855/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3034541/1‑71 (MQ=255) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:3545990/2‑69 (MQ=11) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:2380082/2‑69 (MQ=11) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc < 1:5228399/71‑1 (MQ=255) cgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcg < 1:4007667/71‑1 (MQ=255) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:3970778/1‑71 (MQ=255) gcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:1360600/60‑1 (MQ=11) cATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:1816658/1‑69 (MQ=255) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:3307470/41‑1 (MQ=14) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:1633189/41‑1 (MQ=14) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:2547384/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:3118635/1‑68 (MQ=255) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:4043461/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1834998/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1661702/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:2721273/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3473134/1‑45 (MQ=11) gtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:4971846/1‑50 (MQ=14) tgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:2791128/71‑1 (MQ=255) cgcTGATGCCTGATGTGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa < 1:2414668/70‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1277350/67‑1 (MQ=255) gcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:14595/71‑1 (MQ=255) ctgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:4647595/70‑1 (MQ=255) cctatgcctgatgcgacGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:1427658‑M1/58‑20 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataat > 1:3498067/1‑71 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:1437379‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta > 1:314186‑M1/3‑41 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta > 1:875263‑M1/3‑41 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1903227‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1004805‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:2101907‑M1/2‑40 (MQ=255) gatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATg > 1:1984470/1‑71 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc > 1:2946535‑M1/1‑39 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc > 1:1415165‑M1/1‑39 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:4187097/39‑1 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3815614/71‑1 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:88171/1‑47 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:1887819/54‑1 (MQ=255) tgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:2198426/69‑1 (MQ=255) tgatgcGACGCTAACGCGTCTTATCATGCCTACAAATcgc < 1:1517671/40‑3 (MQ=14) tgatgcGACGCTAACGCGTCTTATCATGCCTACAAATcgc < 1:5430110/40‑3 (MQ=14) gatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:5660260‑M1/52‑20 (MQ=255) gatgcGACGCTGAAGCGTCTTATCAAGCCTACAtagcactgccacgtatgtttacaccgcatccggcata > 1:818729‑M1/1‑33 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:1062898/1‑69 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg > 1:3836249/1‑71 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:713490‑M1/70‑39 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcac > 1:4033205‑M1/1‑32 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2048094‑M1/69‑39 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:4048171‑M1/69‑39 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2484138‑M1/69‑39 (MQ=255) gcGACGCTAACGCGTCTTATCAGCCCTACAAATTCTCACGCCATCCGTAGGCCGGATAAGGCGTTCAcgcc > 1:3373977/1‑71 (MQ=9) cGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGt < 1:421325/47‑1 (MQ=255) gACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaaca > 1:1857692‑M1/1‑28 (MQ=255) aCGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:5142139/50‑1 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacac < 1:2096420‑M1/71‑45 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaa < 1:1281470‑M1/68‑42 (MQ=255) cGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCAt > 1:4647210/1‑71 (MQ=255) gCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:4238763/1‑37 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:1281187/1‑64 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg < 1:3770558‑M1/71‑48 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:2391419‑M1/63‑40 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta < 1:5457551‑M1/45‑22 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta < 1:433358‑M1/45‑22 (MQ=255) tGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1671084/46‑1 (MQ=255) gACGCGACTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGa > 1:732620/1‑71 (MQ=255) aagcgtcTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcac > 1:774384‑M1/3‑21 (MQ=255) aagcgtcTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgca < 1:4692444‑M1/68‑50 (MQ=255) agcgtCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcac < 1:3150977‑M1/69‑51 (MQ=255) gcgTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcactt > 1:1562269‑M1/1‑19 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3094885/53‑1 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3572125/53‑1 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3274196/53‑1 (MQ=255) gTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTg > 1:2133690/1‑70 (MQ=255) tCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:5660959‑M1/55‑40 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaa < 1:4840597/71‑1 (MQ=255) cTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcactttg > 1:4855398‑M1/1‑15 (MQ=255) cTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:5284462/40‑1 (MQ=255) ttATCATGCCTACAtagcactgccacgtatgtttaca > 1:4214843‑M1/1‑14 (MQ=255) ttATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3216099/49‑1 (MQ=255) ttATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:5372232/1‑51 (MQ=255) ttATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa > 1:123280/1‑71 (MQ=255) ttATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAAAGCGTTc > 1:3778498/1‑50 (MQ=255) tATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAAt > 1:4034852/1‑71 (MQ=255) aTCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa < 1:3537942/69‑1 (MQ=255) aTCATGCCTAC‑AATCTGCACCCGAACCGTAGGCCGAATAATGCGTTCACGCCACATCCGACCTGAAAATTc > 1:4937648/1‑71 (MQ=21) aTCATGCCTAC‑AATCTGCACCCGAACCGTAGGCCGAATAATGCGTTCACGCCACATCCGACCTGAAAATTc > 1:3826570/1‑71 (MQ=21) aTCATGACTACAAATCTGTACGCGAACCGTAGGCCGa < 1:1461573/37‑1 (MQ=255) tCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCg < 1:5591709/59‑1 (MQ=255) tCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa < 1:3674492/68‑1 (MQ=255) aTGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaa < 1:3903671/65‑1 (MQ=255) aTGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCt > 1:3423540/1‑70 (MQ=255) tGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCTTa < 1:2603975/71‑1 (MQ=255) | TAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCTTA > Exported/222963‑223100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |