| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 223,627 | T→A | 74.4% | intergenic (+47/+10) | prfH → / ← pepD | predicted peptide chain release factor/aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 223,627 | 0 | T | A | 74.4% | 64.0 / 23.3 | 39 | intergenic (+47/+10) | prfH/pepD | predicted peptide chain release factor/aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base A (12/17); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
ATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAC > Exported/223560‑223689 | aTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCatat > 1:513021/1‑70 (MQ=255)aTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCaaat > 1:1405190/1‑70 (MQ=255) gCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCaaa < 1:660179/71‑1 (MQ=255) ttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAaataat > 1:4802136/1‑71 (MQ=255) ttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa < 1:1876211/70‑1 (MQ=255) tATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAaataa > 1:3172538/1‑69 (MQ=255) tATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat < 1:5349290/70‑1 (MQ=255) tCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc < 1:1142462/71‑1 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATc < 1:2138658/53‑1 (MQ=255) aTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGa < 1:1167490/70‑1 (MQ=255) aaGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAttt < 1:4453716/71‑1 (MQ=255) aaGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAttt > 1:2846230/1‑71 (MQ=255) aGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAttt < 1:525247/70‑1 (MQ=255) gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAt < 1:435002/66‑1 (MQ=255) ccGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGt < 1:5091499/69‑1 (MQ=255) ccGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAAtt < 1:2889224/43‑1 (MQ=255) ccGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGtt < 1:803871/70‑1 (MQ=255) cGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:1241154/71‑1 (MQ=255) cGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:1785425/71‑1 (MQ=255) cGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:5318717/71‑1 (MQ=255) ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGtt < 1:1319656/68‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt > 1:5414332/1‑71 (MQ=255) tggCGTTTAATCGCCTTCCGGCAGTAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt > 1:3658445/2‑69 (MQ=25) tggCGTTTAATCGCCTTCCGGCAGTAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcag > 1:5426807/2‑68 (MQ=25) aGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtca < 1:4434603/71‑1 (MQ=255) aGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGaa > 1:4945021/1‑50 (MQ=255) gCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagc < 1:2478385/71‑1 (MQ=255) cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagca < 1:3647297/71‑1 (MQ=255) cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagca < 1:1178868/71‑1 (MQ=255) aTTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagc < 1:1453631/68‑1 (MQ=255) tAAACGCCATCCGGCAGCAAATCAAATAATTACTTc < 1:4733738/36‑1 (MQ=255) tAAACGCCATCCGGCAGCAAATCAAATAATTACTTc < 1:874660/36‑1 (MQ=255) tAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagca > 1:5023277/1‑55 (MQ=255) tAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAgtgt > 1:499984/1‑71 (MQ=255) aaCGCCATCCGGCAGCAAATCAAATAATTACTTCGc > 1:2138884/1‑36 (MQ=255) aaCGCCATCCGGCAGCAAATCAAATAATTACTTCGc > 1:5603102/1‑36 (MQ=255) aaCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTc > 1:5139722/1‑70 (MQ=255) cGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagcag > 1:2450013/1‑57 (MQ=255) cGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAc > 1:476510/1‑71 (MQ=255) ggCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt > 1:568237/1‑50 (MQ=255) gcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtcag < 1:3017110/49‑1 (MQ=255) | ATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAC > Exported/223560‑223689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |