| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,299,487 | G→A | 62.0% | intergenic (‑412/+78) | cobU ← / ← yeeX | bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,299,487 | 0 | G | A | 62.0% | 33.4 / 47.6 | 50 | intergenic (‑412/+78) | cobU/yeeX | bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/6); new base A (15/16); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAA > Exported/1299419‑1299556 | ttGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggat > 1:2886363/1‑71 (MQ=255) acaacGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACg > 1:2418501/1‑71 (MQ=255) gAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAg < 1:4801208/71‑1 (MQ=255) aTCCGGTATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGAt > 1:736262/1‑68 (MQ=255) aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggat < 1:4214647/48‑1 (MQ=255) aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACCGTg > 1:4438504/1‑58 (MQ=255) ggAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATCATGACGGTGATACACAGATAAGAATc < 1:3470771/71‑1 (MQ=255) cGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaat < 1:2815251/71‑1 (MQ=255) aCTATAATTTCGATTGGTTCTCGGATGAGGCtgatga < 1:734875/37‑1 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGa > 1:4676791/3‑41 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAg < 1:1265391/51‑1 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAg < 1:16855/51‑1 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagca > 1:4760695/3‑65 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagca > 1:1578557/3‑65 (MQ=255) gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAgg > 1:3712144/3‑71 (MQ=255) ttaATTTCGATTGGTTCTCGGATGAGAATGATGACgg < 1:1878994/36‑1 (MQ=255) taATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGt > 1:3682244/1‑71 (MQ=255) aaTTTCGATTGGTTCTCGGATGAGAATGATGCCGGTg > 1:5558383/1‑37 (MQ=255) tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATa < 1:4485575/38‑1 (MQ=255) tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:1576840/51‑1 (MQ=255) tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc < 1:4329047/71‑1 (MQ=255) ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1891705/1‑71 (MQ=255) ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1879182/1‑71 (MQ=255) ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:4659974/1‑71 (MQ=255) ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1138704/1‑71 (MQ=255) tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:5229794/1‑42 (MQ=255) tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:4813716/1‑42 (MQ=255) tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:1811707/1‑42 (MQ=255) cGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatga > 1:3828495/1‑61 (MQ=255) aTTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGc < 1:3413670/71‑1 (MQ=255) aTTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGc < 1:2834904/71‑1 (MQ=255) ttGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCa < 1:1119972/71‑1 (MQ=255) tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc > 1:1126321/1‑64 (MQ=255) tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa > 1:668830/1‑71 (MQ=255) tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCa < 1:4331363/70‑1 (MQ=255) ggTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa < 1:3194197/70‑1 (MQ=255) ggTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa < 1:776394/70‑1 (MQ=255) ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:2492974/1‑57 (MQ=255) ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:3507679/1‑57 (MQ=255) ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:3660162/1‑57 (MQ=255) ggTTCTCGGATGAGAATGATGACGGTGATACAAAGATAAGAATcaatgagcaatgag > 1:4400970/1‑57 (MQ=255) ttCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:5176984/41‑1 (MQ=255) ttCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:3817267/41‑1 (MQ=255) tctcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:4965426/1‑71 (MQ=255) tctcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:1631714/1‑71 (MQ=255) ctcGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:4603600/1‑70 (MQ=255) ctcGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt < 1:325468/70‑1 (MQ=255) cGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaa > 1:275216/1‑47 (MQ=255) cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:2150772/38‑1 (MQ=255) cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:2144172/38‑1 (MQ=255) cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:193263/38‑1 (MQ=255) cGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCg > 1:3113920/1‑59 (MQ=255) gatgaggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCAtt < 1:1463320/70‑1 (MQ=255) gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:3938001/45‑1 (MQ=255) gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:778066/45‑1 (MQ=255) gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:2539479/45‑1 (MQ=255) gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:4382338/45‑1 (MQ=255) catgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:5486510/44‑1 (MQ=255) atgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc < 1:3144439/54‑1 (MQ=255) atgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGa < 1:2588942/71‑1 (MQ=255) tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa < 1:3050738/71‑1 (MQ=255) tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa < 1:1902230/71‑1 (MQ=255) tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGa < 1:2489490/70‑1 (MQ=255) gagaatgaTGACGGTGATACACAGATAAGAATcaa > 1:1941911/1‑35 (MQ=255) gagaatgaTGACGGTGATACACAGATAAGAATcaa > 1:2783634/1‑35 (MQ=255) gagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTg < 1:2689362/51‑1 (MQ=255) gagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa > 1:867047/1‑70 (MQ=255) ggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATaa < 1:2367832/71‑1 (MQ=255) | TTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAA > Exported/1299419‑1299556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |