| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,309,751 | C→T | 29.7% | V81I (GTC→ATC) | yefM ← | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,309,751 | 0 | C | T | 29.7% | 46.6 / 23.3 | 37 | V81I (GTC→ATC) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base C (14/12); new base T (5/6); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGG > Exported/1309681‑1309821 | cTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGAc > 1:3243563/1‑71 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:1593833/1‑71 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:2544970/1‑71 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:1707546/1‑71 (MQ=255) taAGGCTACGCTAGCGTATCAAAAATGACAATTGATTCTATGAATGAATCTGTTCAATGACGTCCt < 1:100302/66‑1 (MQ=255) aaGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCg > 1:3075804/1‑71 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGcc > 1:4816987/1‑64 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:282512/1‑70 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:1602073/1‑70 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:3920503/1‑70 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAtt > 1:4147445/1‑69 (MQ=255) tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTc < 1:2282396/71‑1 (MQ=255) gACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTc < 1:4301870/44‑1 (MQ=255) aCAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCg < 1:1675075/71‑1 (MQ=255) cAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGa < 1:2773632/71‑1 (MQ=255) cTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGa > 1:4887146/1‑66 (MQ=255) tgaatgaaTCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATc > 1:5525881/1‑70 (MQ=255) tgaatgaaTCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCa < 1:2355566/71‑1 (MQ=255) aatCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTcc < 1:1021725/71‑1 (MQ=255) tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:3540320/64‑1 (MQ=255) tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:1820319/64‑1 (MQ=255) tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:3998463/64‑1 (MQ=255) tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATATc > 1:1311772/1‑39 (MQ=255) actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGc > 1:2872641/3‑70 (MQ=255) actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:4832637/3‑71 (MQ=255) actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:4003314/3‑71 (MQ=255) actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:1861607/3‑71 (MQ=255) ttCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCt < 1:993843/42‑1 (MQ=255) ttCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCt < 1:712842/42‑1 (MQ=255) tCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:2853134/40‑1 (MQ=255) tCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:5224612/40‑1 (MQ=255) cAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:4518319/39‑1 (MQ=255) aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGtt < 1:946480/69‑1 (MQ=255) aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAg < 1:4862433/71‑1 (MQ=255) aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAg < 1:2187059/71‑1 (MQ=255) aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTACGGGCGtt > 1:5191849/1‑69 (MQ=255) atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:1902720/41‑1 (MQ=255) atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:3847276/41‑1 (MQ=255) atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:2197681/41‑1 (MQ=255) atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGt < 1:2612528/49‑1 (MQ=255) tGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCg > 1:3194440/1‑71 (MQ=255) gACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgg > 1:4129340/1‑71 (MQ=255) cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:42893/71‑1 (MQ=255) cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:1862645/71‑1 (MQ=255) cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:2122161/71‑1 (MQ=255) | CTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGG > Exported/1309681‑1309821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |