| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,314,024 | T→C | 5.6% | C93C (TGT→TGC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,314,024 | 0 | T | C | 5.6% | 358.8 / 7.8 | 124 | C93C (TGT→TGC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (63/54); new base C (4/3); total (67/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
TGGCCCGCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGG > Exported/1313955‑1314091 | tGGCCCGCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTc > 1:3224690/1‑71 (MQ=255) gCCCGCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCg < 1:603928/71‑1 (MQ=255) gCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAcc > 1:4603229/1‑71 (MQ=255) gCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAcc > 1:4125639/1‑71 (MQ=255) gCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAcc > 1:1524076/1‑71 (MQ=255) aCCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc > 1:1008391/1‑63 (MQ=255) aCCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGccc > 1:396848/1‑71 (MQ=255) ccTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGccc > 1:2120648/1‑70 (MQ=255) ccTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCccg > 1:1432718/1‑71 (MQ=255) atgatgCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGt > 1:5452984/1‑54 (MQ=255) tgCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAg > 1:2962933/1‑71 (MQ=255) tgCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAg > 1:5673214/1‑71 (MQ=255) tgCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAg > 1:5111757/1‑71 (MQ=255) gCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCccgc > 1:4541811/1‑63 (MQ=255) cAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGt < 1:5373365/48‑1 (MQ=255) aGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc < 1:161713/51‑1 (MQ=255) aGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc > 1:3440324/1‑71 (MQ=255) aGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:2031111/71‑1 (MQ=255) aTCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGAt < 1:5085510/63‑1 (MQ=255) cTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCg > 1:4756069/1‑46 (MQ=255) cTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGa > 1:5646913/1‑60 (MQ=255) cTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACt > 1:5412058/1‑71 (MQ=255) cTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACt > 1:708315/1‑71 (MQ=255) ttCACCTAGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCt < 1:674097/50‑1 (MQ=255) cACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGcc > 1:1384773/1‑71 (MQ=255) cACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGc > 1:4664930/1‑70 (MQ=255) aCCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc > 1:1276970/1‑43 (MQ=255) aCCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAcc > 1:4989827/1‑46 (MQ=255) ccTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGt < 1:3551807/71‑1 (MQ=255) cTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc < 1:1985587/41‑1 (MQ=255) cTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc < 1:1108949/41‑1 (MQ=255) cTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGc < 1:3363107/41‑1 (MQ=255) tCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATg < 1:866335/55‑1 (MQ=255) tCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATg < 1:1860455/55‑1 (MQ=255) cGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAc > 1:3507951/1‑41 (MQ=255) cGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAc > 1:1558988/1‑41 (MQ=255) cGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCAc > 1:153819/1‑41 (MQ=255) gCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAg > 1:2691722/1‑55 (MQ=255) gCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCTTAAGc > 1:4892024/1‑71 (MQ=255) gCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTaa < 1:746857/69‑1 (MQ=255) gCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGc > 1:2473536/1‑71 (MQ=255) gCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGc > 1:3199475/1‑71 (MQ=255) aaGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGcc < 1:3162866/70‑1 (MQ=255) aaGGCGTACAGTTTGATGAAGTGCTGATTTGCCCacac > 1:2408007/1‑38 (MQ=37) aaGGCGTACAGTTTGATGAAGTGCTGATTTGCCCacac > 1:3867441/1‑38 (MQ=37) aaGGCGTACAGTTTGATGAAGTGCTGATTTGCCCacac > 1:1777237/1‑38 (MQ=37) aaGGCGTACAGTTTGATGAAGTGCTGATTTGCCCacac > 1:5576554/1‑38 (MQ=37) aGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCg > 1:222643/1‑70 (MQ=255) aGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCg > 1:3488733/1‑70 (MQ=255) aGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:2587966/71‑1 (MQ=255) aGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:3795436/71‑1 (MQ=255) cGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:2482127/52‑1 (MQ=255) tACAGTTTTATGAAGTGCTGATTTGCCCACACCTGCccgccg < 1:4746706/42‑1 (MQ=37) tACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCccg < 1:5304967/39‑1 (MQ=255) tACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCccg < 1:347704/39‑1 (MQ=255) tACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCccg < 1:425818/39‑1 (MQ=255) tACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTg < 1:3510871/55‑1 (MQ=255) tACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTg < 1:1376026/55‑1 (MQ=255) tACAGTTTGATGAAGTGCTGATTTGCCCACACCTGCccgccg < 1:2667646/42‑1 (MQ=38) cAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGAc > 1:2225128/1‑51 (MQ=255) cAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACt > 1:484266/1‑52 (MQ=255) cAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTaa > 1:1189806/1‑70 (MQ=255) cAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTaa > 1:4232045/1‑70 (MQ=255) cAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTaa < 1:256503/70‑1 (MQ=255) gTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGAt < 1:444928/40‑1 (MQ=255) gTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:1017020/46‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGAt < 1:5429820/39‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:4552447/45‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:1361352/45‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:1402156/45‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGc < 1:5188555/45‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACt > 1:4452839/1‑71 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACt < 1:1574811/71‑1 (MQ=255) tttGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACt > 1:5007386/1‑71 (MQ=255) tgatgaAGTGCTGATTTGTCCGCACCTGCCCTCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtgg > 1:5468018/1‑71 (MQ=255) tgatgaAGTGCTGATTTGTCCGCACCTGCCCGCCGa > 1:39897/1‑36 (MQ=255) tgatgaAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtgg > 1:1729615/1‑71 (MQ=255) tgaAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtggtgg < 1:772607/71‑1 (MQ=255) tgaAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtggtgg < 1:472157/71‑1 (MQ=255) aaGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGaa > 1:5195929/1‑71 (MQ=255) gTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGc < 1:3737855/49‑1 (MQ=255) gTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGc < 1:2773685/49‑1 (MQ=255) tGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:717272/51‑1 (MQ=255) tGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:1434376/51‑1 (MQ=255) tGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTACCGTAAGCCGa < 1:1094074/51‑1 (MQ=255) cTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTa > 1:3418943/1‑71 (MQ=255) gATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtgg > 1:4512102/1‑59 (MQ=255) aTTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:1892955/46‑1 (MQ=255) aTTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:2707126/46‑1 (MQ=255) aTTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:225682/46‑1 (MQ=255) aTTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCt < 1:1669942/71‑1 (MQ=255) tttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGa < 1:2053940/45‑1 (MQ=255) tttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtgg > 1:696891/1‑57 (MQ=255) tttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACtgg > 1:1747899/1‑57 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAACCTGGTGGATCGTTATCTg > 1:1026396/1‑70 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTgg > 1:4677623/1‑71 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTgg > 1:1217658/1‑71 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTgg > 1:4077122/1‑71 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTgg > 1:1640232/1‑71 (MQ=255) ttGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTg > 1:2106932/1‑70 (MQ=255) | TGGCCCGCACAACCTGATGATGCAGATCTTCACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGG > Exported/1313955‑1314091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |