| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,390,615 | A→G | 71.7% | Q249Q (CAA→CAG) | nfo → | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,390,615 | 0 | A | G | 71.7% | 178.8 / 80.5 | 120 | Q249Q (CAA→CAG) | nfo | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Reads supporting (aligned to +/- strand): ref base A (17/17); new base G (42/44); total (59/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
CGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAG > Exported/1390547‑1390681 | cgcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:2416076/1‑71 (MQ=255) gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:5041463/1‑70 (MQ=255) tGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGAcc > 1:2903703/1‑71 (MQ=255) aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGt > 1:498833/1‑71 (MQ=255) aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGt > 1:576348/1‑71 (MQ=255) ccGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGtt < 1:3903613/71‑1 (MQ=255) gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTc < 1:3889519/71‑1 (MQ=255) gCCATCATAGCCTCGGAGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTc < 1:2305848/71‑1 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc > 1:786291/1‑70 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc > 1:1728552/1‑70 (MQ=255) atcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGAc < 1:2718316/71‑1 (MQ=255) tcatAGCCTCGTTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:5527570/68‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGttt > 1:5138435/1‑64 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:1330758/1‑67 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:3066369/70‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:1225596/70‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:3197701/70‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:4004741/70‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg > 1:750959/1‑70 (MQ=255) tAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa > 1:1300139/1‑71 (MQ=255) gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCAt > 1:1660334/1‑70 (MQ=255) cTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTcc < 1:27343/71‑1 (MQ=255) tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCg > 1:5316967/1‑71 (MQ=255) tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCg > 1:3450932/1‑71 (MQ=255) tCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCg > 1:5581133/1‑71 (MQ=255) ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCg < 1:3146161/69‑1 (MQ=255) ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCg < 1:2962635/69‑1 (MQ=255) ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGc < 1:813370/70‑1 (MQ=255) gTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTg < 1:4979206/71‑1 (MQ=255) gTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTg < 1:735100/71‑1 (MQ=255) gAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGAt > 1:4717645/1‑71 (MQ=255) aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATc > 1:723889/1‑71 (MQ=255) ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa > 1:1017968/1‑53 (MQ=255) ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGAc < 1:1585931/54‑1 (MQ=255) gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc < 1:1228278/71‑1 (MQ=255) cAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCg < 1:3199164/71‑1 (MQ=255) cAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCg < 1:4049172/71‑1 (MQ=255) cAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCg < 1:1500872/71‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCg < 1:1681627/70‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:2901670/71‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:5509785/71‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:3782188/71‑1 (MQ=255) atacCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:5103647/71‑1 (MQ=255) tatCGGTCATGATGCTTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTcc < 1:2392664/57‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGga < 1:700072/36‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa < 1:760549/48‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa < 1:4196756/48‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:856033/1‑48 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:1494487/1‑48 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa < 1:1839163/48‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaga > 1:4373681/1‑69 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:4558910/1‑71 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:2449868/1‑35 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:4757218/1‑35 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa < 1:1891539/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:3050954/45‑1 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:2784693/1‑46 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc < 1:5107569/70‑1 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:4496410/1‑70 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa > 1:5283917/1‑68 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa > 1:2938968/1‑68 (MQ=255) gTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc < 1:5574596/71‑1 (MQ=255) tgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGAt > 1:5663160/1‑54 (MQ=255) gatgCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:5307203/36‑1 (MQ=255) gatgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTc > 1:1104639/1‑46 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:4437023/1‑35 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:328129/1‑35 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:42690/1‑35 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:4956359/1‑35 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:3374223/1‑35 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:4317806/71‑1 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:2004999/71‑1 (MQ=255) cGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCgg > 1:1657547/1‑69 (MQ=255) ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTcc < 1:4896837/41‑1 (MQ=255) ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTcc < 1:3162277/41‑1 (MQ=255) ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCaa < 1:505533/62‑1 (MQ=255) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCAtt > 1:1678386/1‑37 (MQ=255) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCt > 1:4733310/1‑42 (MQ=255) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCt > 1:1793592/1‑42 (MQ=255) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCt > 1:5409857/1‑42 (MQ=255) cGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:5354652/70‑1 (MQ=255) cGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg > 1:5291433/1‑71 (MQ=255) cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg > 1:118005/1‑71 (MQ=255) tGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTggg > 1:5118190/1‑70 (MQ=255) gATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCag < 1:4833217/71‑1 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:5354149/51‑1 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:2341464/51‑1 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:3851013/51‑1 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:4424241/51‑1 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:3613379/51‑1 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:3122490/1‑43 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCAt > 1:5190526/1‑47 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc > 1:304554/1‑48 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGa > 1:4190338/1‑56 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:4558559/1‑69 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaag < 1:5399835/70‑1 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaag < 1:2257283/70‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:721951/1‑36 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:3319053/1‑36 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:3335036/1‑36 (MQ=255) | CGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAG > Exported/1390547‑1390681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |