Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,395,660 T→A 12.9% A122A (GCT→GCA setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,395,6600TA12.9% 264.0 / 26.1 100A122A (GCT→GCAsetBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base T (45/42);  new base A (6/7);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 103 total aligned reads displayed.

TGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGC  >  Exported/1395591‑1395729
                                                                     |                                                                     
tGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCt                                                                       <  1:2168085/70‑1 (MQ=255)
tGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCt                                                                       <  1:4234191/70‑1 (MQ=255)
 gCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTa                                                                      <  1:2044984/70‑1 (MQ=255)
 gCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTa                                                                      <  1:556707/70‑1 (MQ=255)
 gCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTa                                                                      <  1:1795002/70‑1 (MQ=255)
     ggAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCg                                                                 >  1:5539734/1‑71 (MQ=255)
      gAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAccc                                                                  <  1:981798/69‑1 (MQ=255)
      gAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCg                                                                 >  1:2731759/1‑70 (MQ=255)
       aaTCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGc                                                                >  1:2662203/1‑70 (MQ=255)
        aTCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTa                                                                      >  1:2399441/1‑63 (MQ=255)
           gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAAt                                                            <  1:3231286/70‑1 (MQ=255)
           gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATg                                                           <  1:3005094/71‑1 (MQ=255)
           gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATg                                                           >  1:2356862/1‑71 (MQ=255)
           gCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATg                                                           <  1:489317/71‑1 (MQ=255)
             aaCTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGt                                                          <  1:2204527/70‑1 (MQ=255)
               ctactTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg                                                       <  1:499377/71‑1 (MQ=255)
                 actTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGttt                                                        >  1:3792635/1‑68 (MQ=255)
                 actTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGttt                                                        >  1:3575381/1‑68 (MQ=255)
                 actTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcc                                                     >  1:3536042/1‑71 (MQ=255)
                 actTTGATTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGATCGACCGCTAACCCGCAAATGTttgcc                                                     >  1:114151/1‑71 (MQ=255)
                  ctTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcc                                                     <  1:1871584/70‑1 (MQ=255)
                     tGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTaa                                                                     >  1:1534247/1‑51 (MQ=255)
                     tGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttg                                                 >  1:4764043/1‑71 (MQ=255)
                     tGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgccc                                                    <  1:4020474/68‑1 (MQ=255)
                       ttttGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgcc                                               <  1:2629633/71‑1 (MQ=255)
                          tGCTATTCGTTGGCGTCTTTCTTATCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCACTTGCCCGt                                            >  1:5608040/1‑71 (MQ=255)
                          tGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGt                                                          >  1:2045115/1‑57 (MQ=255)
                          tGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGt                                                          >  1:2945977/1‑57 (MQ=255)
                          tGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg                                                       >  1:518647/1‑60 (MQ=255)
                           gCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAcc                                                                   >  1:1204837/1‑47 (MQ=255)
                           gCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGt                                            <  1:3121649/70‑1 (MQ=255)
                            cTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGa                                          <  1:3905033/71‑1 (MQ=255)
                             tATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         >  1:1225743/1‑71 (MQ=255)
                               ttCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:1181248/69‑1 (MQ=255)
                                tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                      >  1:3798946/1‑71 (MQ=255)
                                tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                      >  1:673789/1‑71 (MQ=255)
                                tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                      >  1:3063987/1‑71 (MQ=255)
                                 cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATg                                     >  1:2272248/1‑71 (MQ=255)
                                 cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATg                                     >  1:4623018/1‑71 (MQ=255)
                                  gTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATg                                                           <  1:1728812/48‑1 (MQ=255)
                                  gTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATg                                                           <  1:3163148/48‑1 (MQ=255)
                                    tGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGcc                                   >  1:855280/1‑70 (MQ=255)
                                     ggCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGt                                                          >  1:3548211/1‑46 (MQ=255)
                                      gCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:2916648/62‑1 (MQ=255)
                                       cGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGAc                                >  1:528450/1‑70 (MQ=255)
                                        gTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa                              <  1:1485890/71‑1 (MQ=255)
                                         tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:4375179/59‑1 (MQ=255)
                                         tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:101739/59‑1 (MQ=255)
                                         tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:1493566/59‑1 (MQ=255)
                                         tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         <  1:3232235/59‑1 (MQ=255)
                                           tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGttt                                                        <  1:15082/42‑1 (MQ=255)
                                           tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGc                                    <  1:5233794/62‑1 (MQ=255)
                                           tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAAc                           >  1:4570821/1‑71 (MQ=255)
                                            ttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa                              <  1:314623/67‑1 (MQ=255)
                                            ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTg                                           <  1:520729/54‑1 (MQ=255)
                                            ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTg                                           <  1:527433/54‑1 (MQ=255)
                                            ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACagaa                            >  1:5021657/1‑69 (MQ=255)
                                            ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACagaa                            >  1:1648555/1‑69 (MQ=255)
                                             tcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAATCCg                         >  1:5358291/1‑71 (MQ=255)
                                                 agcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                         >  1:4240929/1‑51 (MQ=255)
                                                  gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGt                    <  1:2887441/71‑1 (MQ=255)
                                                  gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt                    <  1:5205782/71‑1 (MQ=255)
                                                   cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATTCCGACAAAACCGGACGTg                   <  1:3663542/71‑1 (MQ=255)
                                                   cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                   <  1:1868552/71‑1 (MQ=255)
                                                     gcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAtag                                    <  1:1653959/52‑3 (MQ=255)
                                                      cTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgc                                                <  1:5360100/39‑1 (MQ=255)
                                                      cTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgc                                                <  1:3445092/39‑1 (MQ=255)
                                                        ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCg                                             >  1:1211650/1‑40 (MQ=255)
                                                        ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                        >  1:2319935/1‑45 (MQ=255)
                                                        ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                        >  1:904067/1‑45 (MQ=255)
                                                         tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                        >  1:4901421/1‑44 (MQ=255)
                                                         tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc               <  1:4069707/69‑1 (MQ=255)
                                                         tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc               <  1:2859038/69‑1 (MQ=255)
                                                          ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg             <  1:2464191/70‑1 (MQ=255)
                                                          ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg             <  1:655697/70‑1 (MQ=255)
                                                          ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg             <  1:3938887/70‑1 (MQ=255)
                                                           gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg           <  1:2601181/71‑1 (MQ=255)
                                                            cTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACg                     <  1:1969996/60‑1 (MQ=255)
                                                             tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt         >  1:1455267/1‑71 (MQ=255)
                                                             tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt         >  1:4926446/1‑71 (MQ=255)
                                                              cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                   >  1:3630451/1‑60 (MQ=255)
                                                              cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg        >  1:3794624/1‑71 (MQ=255)
                                                                aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACa                                       >  1:2103780/1‑38 (MQ=255)
                                                                aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACa                                       >  1:319160/1‑38 (MQ=255)
                                                                aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                            >  1:3869454/1‑49 (MQ=255)
                                                                aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                            >  1:5334500/1‑49 (MQ=255)
                                                                aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                            >  1:5340959/1‑49 (MQ=255)
                                                                aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      <  1:1754783/71‑1 (MQ=255)
                                                                aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      <  1:1246216/71‑1 (MQ=255)
                                                                aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      <  1:1748280/71‑1 (MQ=255)
                                                                aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      <  1:5499068/71‑1 (MQ=255)
                                                                 ccGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAAca                          >  1:2689226/1‑49 (MQ=255)
                                                                  cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAggcc                      >  1:3379320/1‑51 (MQ=255)
                                                                  cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      >  1:5679529/1‑69 (MQ=255)
                                                                  cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt      >  1:4275324/1‑69 (MQ=255)
                                                                   gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg   >  1:2190858/1‑71 (MQ=255)
                                                                    cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc  <  1:1596494/71‑1 (MQ=255)
                                                                    cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc  >  1:3744981/1‑71 (MQ=255)
                                                                    cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc  >  1:449811/1‑71 (MQ=255)
                                                                     tAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaa                             >  1:2489677/1‑43 (MQ=255)
                                                                     |                                                                     
TGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGC  >  Exported/1395591‑1395729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: