Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,398,585 T→G 18.6% A303A (GCT→GCG yeiQ → predicted dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,398,5850TG18.6% 188.1 / 47.4 97A303A (GCT→GCGyeiQpredicted dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (31/48);  new base G (7/11);  total (38/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 104 total aligned reads displayed.

GTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCG  >  Exported/1398517‑1398655
                                                                    |                                                                      
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                                       <  1:4777362/70‑1 (MQ=255)
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                                       <  1:5593535/70‑1 (MQ=255)
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      >  1:4255604/1‑71 (MQ=255)
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      >  1:3027454/1‑71 (MQ=255)
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      <  1:5051443/71‑1 (MQ=255)
gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      <  1:5078414/71‑1 (MQ=255)
   agcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                                                    <  1:2354516/67‑1 (MQ=255)
   aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCt                                                                   <  1:5027712/71‑1 (MQ=255)
    gcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCt                                                                   <  1:3224720/68‑1 (MQ=255)
     cgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                                                    <  1:1424359/67‑1 (MQ=255)
      gatgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg                                                                 <  1:5159011/70‑1 (MQ=255)
        tgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGtt                                                              <  1:4385565/71‑1 (MQ=255)
          tCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                                       <  1:4665547/60‑1 (MQ=255)
           ccTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                                                           >  1:5546970/1‑71 (MQ=255)
             tGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      <  1:5057520/58‑1 (MQ=255)
               ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                                                        <  1:3320876/70‑1 (MQ=255)
               ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                                         <  1:5654675/69‑1 (MQ=255)
                cATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                                                           >  1:3515975/1‑66 (MQ=255)
                   gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                                                  <  1:2749945/56‑1 (MQ=255)
                   gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                                                  <  1:1739868/56‑1 (MQ=255)
                   atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                                                   >  1:1029373/3‑71 (MQ=255)
                   atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                                                   >  1:5199356/3‑71 (MQ=255)
                    tgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGAt                                                  <  1:1907401/70‑1 (MQ=255)
                    ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                                                  <  1:1049247/71‑1 (MQ=255)
                    ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                                                  <  1:2370704/71‑1 (MQ=255)
                     gAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAtt                                                 >  1:489934/1‑71 (MQ=255)
                        gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCtctc                                                       >  1:879195/1‑62 (MQ=255)
                         tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                                                     >  1:2217285/2‑46 (MQ=16)
                         tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                                                     >  1:756699/2‑46 (MQ=16)
                         agaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc                                             <  1:5531092/71‑1 (MQ=255)
                         agaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc                                             <  1:2707655/71‑1 (MQ=255)
                          gaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc                                             <  1:3547853/70‑1 (MQ=255)
                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                                                        <  1:3071036/58‑1 (MQ=255)
                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                                         <  1:2436884/57‑1 (MQ=255)
                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                                         <  1:1556137/57‑1 (MQ=255)
                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCa                                           >  1:4584046/1‑71 (MQ=255)
                            tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                                          <  1:3566817/71‑1 (MQ=255)
                            tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                                          <  1:3302335/71‑1 (MQ=255)
                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                                                >  1:3509646/1‑48 (MQ=255)
                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                                                >  1:2800551/1‑48 (MQ=255)
                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                                          <  1:2486661/70‑1 (MQ=255)
                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCt                                                                        >  1:103924/1‑39 (MQ=255)
                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt                                                                     >  1:4186710/1‑42 (MQ=255)
                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                                        >  1:4494058/1‑71 (MQ=255)
                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                                        >  1:4617564/1‑71 (MQ=255)
                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                                                  <  1:2001653/59‑1 (MQ=255)
                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                                                  <  1:729386/59‑1 (MQ=255)
                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTc                                                <  1:2563694/61‑1 (MQ=255)
                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                                        >  1:278798/1‑69 (MQ=255)
                                 ctgctgATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                                       <  1:923099/37‑1 (MQ=255)
                                 cTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCtc                                                                  <  1:5095352/42‑2 (MQ=12)
                                 cTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAc                                                            <  1:669445/48‑1 (MQ=255)
                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                                      <  1:838642/35‑1 (MQ=255)
                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt                                                                     >  1:3124372/1‑36 (MQ=255)
                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt                                    <  1:4640400/69‑1 (MQ=255)
                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTg                                   <  1:3296696/70‑1 (MQ=255)
                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                                                  <  1:5406986/38‑1 (MQ=255)
                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                                                  <  1:4890018/38‑1 (MQ=255)
                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                                                  <  1:751561/38‑1 (MQ=255)
                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGa                                  <  1:4052202/70‑1 (MQ=255)
                                      taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                                           <  1:3865900/43‑1 (MQ=16)
                                      taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                                           <  1:2209461/43‑1 (MQ=16)
                                      taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                                           <  1:3023955/43‑1 (MQ=16)
                                      taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                                           <  1:319173/43‑1 (MQ=16)
                                      taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                                           <  1:43793/43‑1 (MQ=16)
                                      gATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGAtt                                <  1:839277/71‑1 (MQ=255)
                                       aTGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg                               <  1:4027587/71‑1 (MQ=255)
                                        tGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACctc                                                         >  1:3064531/1‑44 (MQ=16)
                                        tGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACctc                                                         >  1:4265212/1‑44 (MQ=16)
                                        tGCTGAACGGCAGACACTCTTTTCTGGCGTACCTCGGTTACctc                                                         >  1:2113970/1‑44 (MQ=255)
                                           ttAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTAGCCCATATCAGTGATtgcatg                           >  1:1332273/1‑71 (MQ=255)
                                            tAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCa                                           >  1:884494/1‑54 (MQ=255)
                                             aaTGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatg                           <  1:1182832/69‑1 (MQ=255)
                                              aTGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAg                        >  1:5126400/1‑71 (MQ=255)
                                               tGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc                          <  1:3728052/68‑1 (MQ=255)
                                               tGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc                          <  1:1939867/68‑1 (MQ=255)
                                                     ccACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg                 <  1:2551372/71‑1 (MQ=255)
                                                      cACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgca                         >  1:1060234/1‑62 (MQ=255)
                                                      cACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc                          >  1:208736/1‑61 (MQ=255)
                                                      cACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCgcgc                <  1:2882270/71‑1 (MQ=255)
                                                      cACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCgcgc                <  1:1276337/71‑1 (MQ=255)
                                                        ctctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt              >  1:1836041/1‑71 (MQ=255)
                                                         tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg                       <  1:1137083/61‑1 (MQ=255)
                                                         tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAtt             >  1:1263395/1‑71 (MQ=255)
                                                          ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc                          >  1:2268802/1‑57 (MQ=255)
                                                           ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCg                                               >  1:704466/1‑35 (MQ=255)
                                                           ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCa                                           >  1:867980/1‑39 (MQ=255)
                                                            tttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAtt             <  1:4557502/68‑1 (MQ=255)
                                                             ttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAtt             >  1:889460/1‑67 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCata                                         >  1:3571500/1‑38 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCata                                         >  1:3874216/1‑38 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc         <  1:5378455/70‑1 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc         <  1:1751731/70‑1 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc         <  1:4390868/70‑1 (MQ=255)
                                                              tctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc         <  1:5522876/70‑1 (MQ=255)
                                                               ctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt                                    >  1:2161392/1‑42 (MQ=255)
                                                               ctcGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGcc        <  1:4857124/70‑1 (MQ=255)
                                                                  gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGAt                                 >  1:5619090/1‑42 (MQ=255)
                                                                    ttATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc                          <  1:777520/47‑1 (MQ=255)
                                                                    ttATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATgccg  >  1:598562/1‑71 (MQ=255)
                                                                    |                                                                      
GTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCG  >  Exported/1398517‑1398655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: