| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,291 | G→A | 12.3% | Y337Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,291 | 0 | G | A | 12.3% | 580.7 / 54.0 | 228 | Y337Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (115/85); new base A (10/18); total (125/103) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 238 total aligned reads displayed.
GAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATA > Exported/1439221‑1439360 | gagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTTGTCCAGCAGGTTGATTTTg < 1:736171/71‑1 (MQ=255)gagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg > 1:2556152/1‑71 (MQ=255) gCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg > 1:3067279/1‑69 (MQ=255) cTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg > 1:4458924/1‑71 (MQ=255) ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:4031249/71‑1 (MQ=255) aTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:5022752/1‑71 (MQ=255) tCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:38354/1‑70 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:5355698/66‑1 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa > 1:1183250/1‑53 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa > 1:2312648/1‑53 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg > 1:5321850/1‑66 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:2351594/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt > 1:4381702/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt < 1:778097/71‑1 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt < 1:2947650/71‑1 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt < 1:4147216/70‑1 (MQ=255) gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt > 1:1636361/1‑71 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:5478143/70‑1 (MQ=255) aGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa > 1:5609555/1‑45 (MQ=255) aGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:3381229/68‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:4415228/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:2661346/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:1584789/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:2061745/1‑70 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:1091368/1‑70 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGCACAt > 1:2442057/1‑61 (MQ=255) gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:4625872/71‑1 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa > 1:5638620/1‑44 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:4316277/1‑50 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc > 1:3105519/1‑55 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc > 1:267152/1‑55 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag < 1:3443245/70‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:3894066/43‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:2777514/44‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta < 1:3003898/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg > 1:1019975/1‑42 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt < 1:253934/54‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3655631/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:225726/1‑70 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:2870853/1‑70 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:3529130/1‑70 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:48516/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:2591226/59‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:4665887/71‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:108110/71‑1 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:5450907/1‑71 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:1678382/1‑71 (MQ=255) gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt > 1:3393273/1‑62 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:1029685/1‑70 (MQ=255) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa < 1:857501/70‑1 (MQ=255) cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca > 1:3199365/1‑71 (MQ=255) catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCca < 1:2721209/66‑1 (MQ=17) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt > 1:3977898/1‑45 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:1868589/1‑47 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:1502786/1‑47 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:5407005/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa > 1:3334123/1‑64 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt < 1:1146107/43‑1 (MQ=21) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:335678/69‑3 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:4728259/69‑3 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:2830113/69‑3 (MQ=17) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt < 1:4061150/44‑1 (MQ=255) tcCAGCAGGTTGATTCTGTAGTCAACGTAGGTGGACATGttttt < 1:2523792/44‑1 (MQ=255) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat > 1:435950/1‑44 (MQ=21) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat > 1:5055669/1‑44 (MQ=21) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:5512792/1‑59 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:2143839/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:2483662/1‑71 (MQ=255) caacagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc < 1:5209956/60‑1 (MQ=14) agcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:4211506/45‑1 (MQ=17) aacagGTTGATTTTATAGTCAACGTAGGAGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:1941922/63‑2 (MQ=12) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:3425900/71‑1 (MQ=255) acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:4093247/63‑2 (MQ=17) cagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacgt < 1:1798618/71‑3 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:2701219/1‑65 (MQ=17) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:4943607/71‑1 (MQ=17) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag < 1:3031492/43‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat < 1:1992361/68‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:1943494/1‑71 (MQ=255) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc < 1:4986682/57‑1 (MQ=14) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc < 1:93082/57‑1 (MQ=14) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1251756/1‑57 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:688004/1‑70 (MQ=255) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGaa > 1:1638300/1‑40 (MQ=14) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAag < 1:3504904/41‑1 (MQ=14) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3398675/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3949071/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:5196568/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:2883354/1‑68 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:1056200/71‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:4959080/71‑1 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:2376032/36‑1 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:2214622/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:3995406/1‑68 (MQ=17) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:4280307/1‑68 (MQ=17) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:1517902/1‑68 (MQ=17) ttttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag > 1:2612646/1‑38 (MQ=255) tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:377474/48‑1 (MQ=14) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:2087892/64‑1 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:4159667/1‑61 (MQ=255) | GAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATA > Exported/1439221‑1439360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |