| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,294 | G→A | 16.4% | D336D (GAC→GAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,294 | 0 | G | A | 16.4% | 542.1 / 77.9 | 225 | D336D (GAC→GAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (101/87); new base A (21/16); total (122/103) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 229 total aligned reads displayed.
CTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCT > Exported/1439224‑1439363 | aCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt > 1:171737/1‑70 (MQ=255) cGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc < 1:5637433/70‑1 (MQ=255) ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg > 1:3940912/1‑69 (MQ=255) ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:4031249/71‑1 (MQ=255) gtgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg > 1:2460578/1‑70 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:1046173/66‑1 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:3768460/66‑1 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:3775057/66‑1 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:5355698/66‑1 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg > 1:5321850/1‑66 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:271094/1‑71 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGATTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:824490/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt > 1:4381702/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt < 1:778097/71‑1 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt > 1:2530451/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt < 1:4147216/70‑1 (MQ=255) gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt > 1:3396512/1‑71 (MQ=255) gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt > 1:1636361/1‑71 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:5478143/70‑1 (MQ=255) cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg > 1:3092386/1‑71 (MQ=255) cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg < 1:2314737/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:3392749/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:2661346/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:2061745/1‑70 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg > 1:2860493/1‑40 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa > 1:2770774/1‑44 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:4316277/1‑50 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag < 1:3443245/70‑1 (MQ=255) gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag > 1:503619/1‑71 (MQ=255) aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:3431505/71‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:3894066/43‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:2667403/43‑1 (MQ=255) tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag < 1:4993626/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:3039062/1‑43 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg > 1:1019975/1‑42 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3655631/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3413120/71‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:4003433/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:4341750/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:4665887/71‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:108110/71‑1 (MQ=255) ggTTGTCGTCCAGCAGTTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg > 1:1873684/1‑56 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:5450907/1‑71 (MQ=255) gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt > 1:3374146/1‑62 (MQ=255) ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2695107/71‑1 (MQ=255) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa > 1:2783014/4‑71 (MQ=18) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa < 1:3520931/68‑1 (MQ=18) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2572929/1‑71 (MQ=255) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:5410912/1‑71 (MQ=255) tcatcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa > 1:2288634/4‑70 (MQ=17) cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca > 1:3199365/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt > 1:3977898/1‑45 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:4390068/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:2913728/1‑71 (MQ=255) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt < 1:1146107/43‑1 (MQ=21) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:4728259/69‑3 (MQ=17) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt < 1:4061150/44‑1 (MQ=255) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat > 1:1058379/1‑44 (MQ=21) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc < 1:1550976/71‑1 (MQ=17) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:5512792/1‑59 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:2143839/1‑71 (MQ=255) cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca < 1:4983761/70‑1 (MQ=17) agcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:4211506/45‑1 (MQ=17) agcagGGTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAgtagta < 1:4868277/53‑1 (MQ=14) aacagGTTGATTTTATAGTCAACGTAGGAGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:1941922/63‑2 (MQ=12) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:3324712/1‑71 (MQ=255) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:1599128/1‑71 (MQ=255) gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:5693118/71‑2 (MQ=17) acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:4093247/63‑2 (MQ=17) cagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacgt < 1:1798618/71‑3 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:1673454/1‑65 (MQ=17) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:5221442/71‑1 (MQ=17) agGTTGATTTTATAGTCAACGTAGGTGGACATGttttta > 1:2596783/1‑38 (MQ=18) ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat < 1:3344538/71‑1 (MQ=255) ggTTGATTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:2978689/41‑1 (MQ=14) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:2416003/41‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:1167466/47‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:2497682/47‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:4643368/51‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat < 1:1992361/68‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:1943494/1‑71 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:151752/1‑57 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:688004/1‑70 (MQ=255) tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa > 1:4956813/1‑57 (MQ=14) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAag < 1:3504904/41‑1 (MQ=14) gATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt < 1:1391926/36‑1 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2546674/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:5196568/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3949071/1‑69 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:894945/71‑1 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:2214622/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:5070657/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:4280307/1‑68 (MQ=17) ttttATAGTCAACGTAGGTGGACATGTTTTTATTGaaa < 1:2357745/38‑2 (MQ=14) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2877197/50‑1 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:2087892/64‑1 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata > 1:4428765/1‑71 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:301854/1‑61 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:4159667/1‑61 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:4759256/1‑64 (MQ=255) | CTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCT > Exported/1439224‑1439363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |