Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,423 A→G 18.7% R293R (CGT→CGC ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4230AG18.7% 259.3 / 45.7 112R293R (CGT→CGCompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (37/54);  new base G (9/12);  total (46/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

Only 100 of 135 total aligned reads displayed.

AGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGG  >  Exported/1439355‑1439493
                                                                    |                                                                      
aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                                      <  1:747491/71‑1 (MQ=255)
aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                                      <  1:2320555/71‑1 (MQ=255)
  gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                                    <  1:135816/71‑1 (MQ=255)
  gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                                    <  1:1458373/71‑1 (MQ=255)
  gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                                    <  1:542678/71‑1 (MQ=255)
    tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                                    <  1:4449484/69‑1 (MQ=255)
    tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa                                                                  <  1:2167186/71‑1 (MQ=255)
     atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc                                                                 >  1:4839122/1‑71 (MQ=255)
        ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGCCCg                                                               >  1:513285/1‑70 (MQ=255)
        ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                               >  1:2203836/1‑70 (MQ=255)
         tcgtcgtcgTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                                                                        >  1:964194/1‑60 (MQ=255)
          cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                               <  1:4221174/68‑1 (MQ=255)
          cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                               <  1:4553075/68‑1 (MQ=255)
          cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                               <  1:1738365/68‑1 (MQ=255)
          cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                             <  1:2506492/70‑1 (MQ=255)
          cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa                                                              <  1:4591558/69‑1 (MQ=255)
           gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTGGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                             <  1:2625254/69‑1 (MQ=255)
           gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                           >  1:3647463/1‑71 (MQ=255)
              gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGa                                                        <  1:1833562/71‑1 (MQ=255)
                cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                      >  1:2020717/1‑71 (MQ=255)
                 gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                      <  1:1064020/70‑1 (MQ=255)
                 gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                     >  1:4348782/1‑71 (MQ=255)
                 gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc                                                      <  1:3198147/70‑1 (MQ=255)
                    gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                   <  1:5136905/70‑1 (MQ=255)
                    gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                   <  1:2769725/70‑1 (MQ=255)
                       aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt                                               >  1:4915708/1‑71 (MQ=255)
                              aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                        <  1:5095140/71‑1 (MQ=255)
                               ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                       >  1:3569540/1‑55 (MQ=255)
                               ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                                       <  1:3138662/71‑1 (MQ=255)
                                 tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                             <  1:2070705/47‑1 (MQ=255)
                                  ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                         >  1:104976/1‑50 (MQ=255)
                                  ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                         >  1:3046131/1‑50 (MQ=255)
                                  ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                         >  1:152100/1‑50 (MQ=255)
                                  ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                    >  1:5083192/1‑71 (MQ=255)
                                  ttttACCTTTAGACTGCAGGTAAGCCAGCGACGGACGCAGACCGAAGTCg                                                         >  1:282336/1‑50 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                                      <  1:957795/36‑1 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc                                           <  1:5545305/63‑1 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                   >  1:359277/1‑71 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                   >  1:3856771/1‑71 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                    <  1:2948643/70‑1 (MQ=255)
                                   tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                    <  1:1406890/70‑1 (MQ=255)
                                     tACCTTTAGATTGCAGGTAAGAGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                               >  1:2668514/1‑57 (MQ=255)
                                     tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa                                         >  1:5181823/1‑63 (MQ=255)
                                     tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc                                  <  1:4635071/70‑1 (MQ=255)
                                      aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACtt                                   >  1:3224646/1‑68 (MQ=18)
                                      aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                 <  1:2125179/70‑2 (MQ=18)
                                      aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                 <  1:229860/70‑1 (MQ=255)
                                       ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                 <  1:2128302/69‑2 (MQ=18)
                                        cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                              >  1:4586901/1‑71 (MQ=255)
                                        cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                              >  1:1664479/1‑71 (MQ=255)
                                          ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                            >  1:2825957/1‑71 (MQ=16)
                                          ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                            >  1:2920638/1‑71 (MQ=255)
                                          ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt                             >  1:640514/1‑70 (MQ=255)
                                           tAGATTGCAGGTAGGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt                             <  1:676837/69‑1 (MQ=12)
                                           tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                            <  1:4497202/70‑1 (MQ=16)
                                           tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                            <  1:3856330/70‑1 (MQ=16)
                                            aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                                 >  1:2225626/1‑48 (MQ=2)
                                            aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                                 >  1:2729144/1‑48 (MQ=2)
                                            aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGttt                           <  1:1728506/70‑2 (MQ=12)
                                            aGATTGCAGGTAAGCAACGGACGGACGCAGACCGAAATCGAACTGATATTGTGCAACAGCTTCGAAGtttt                          >  1:4744142/1‑69 (MQ=17)
                                            aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc                           <  1:3576373/70‑1 (MQ=255)
                                             gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                          <  1:2782426/70‑1 (MQ=255)
                                               cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg                       <  1:839806/71‑1 (MQ=255)
                                                  cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca                                                     >  1:1757761/1‑37 (MQ=255)
                                                    ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt                     <  1:1927208/68‑1 (MQ=255)
                                                    ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg                  <  1:5580003/71‑1 (MQ=255)
                                                     gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                                  <  1:1866340/54‑1 (MQ=9)
                                                     gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt                 >  1:5621379/1‑71 (MQ=12)
                                                     gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                     <  1:5455682/35‑1 (MQ=255)
                                                     gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                     <  1:1653707/35‑1 (MQ=255)
                                                     gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                     <  1:305950/35‑1 (MQ=255)
                                                     gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                         <  1:4577851/63‑1 (MQ=255)
                                                      tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                <  1:1279895/71‑1 (MQ=12)
                                                      tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                <  1:212433/71‑1 (MQ=12)
                                                      tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                <  1:5201831/71‑1 (MQ=12)
                                                      tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                         <  1:5024250/62‑1 (MQ=255)
                                                      tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt                <  1:4205124/71‑1 (MQ=255)
                                                       aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                                     >  1:3181866/1‑49 (MQ=255)
                                                       aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc               >  1:2821390/1‑71 (MQ=255)
                                                        aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg              >  1:3673739/1‑71 (MQ=255)
                                                        aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg              >  1:5202142/1‑71 (MQ=255)
                                                           cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc            >  1:3970357/1‑70 (MQ=255)
                                                           cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc            >  1:4290362/1‑70 (MQ=255)
                                                            aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa          <  1:3738549/71‑1 (MQ=255)
                                                             tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc             <  1:473578/66‑1 (MQ=12)
                                                             gggACGGACGCAGACCGAAGTCTAACTGGTACTGAGCAAc                                        <  1:279155/40‑1 (MQ=255)
                                                             gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                        <  1:4756293/40‑1 (MQ=255)
                                                             gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                        <  1:1054902/40‑1 (MQ=255)
                                                             gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                          <  1:1573870/54‑1 (MQ=255)
                                                              ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                            <  1:525522/51‑1 (MQ=255)
                                                               gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc       <  1:888507/71‑1 (MQ=255)
                                                                acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa          <  1:4511539/67‑1 (MQ=255)
                                                                acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa          <  1:5427483/67‑1 (MQ=255)
                                                                acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc      <  1:477419/71‑1 (MQ=255)
                                                                 cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc            <  1:5247982/64‑1 (MQ=255)
                                                                  ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                         <  1:5569083/50‑1 (MQ=255)
                                                                  ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg    <  1:5348626/71‑1 (MQ=255)
                                                                    acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg  <  1:4712528/71‑1 (MQ=255)
                                                                    acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg  <  1:4695601/71‑1 (MQ=255)
                                                                    acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg   >  1:1440325/1‑70 (MQ=255)
                                                                    |                                                                      
AGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGG  >  Exported/1439355‑1439493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: