Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,813 G→A 15.8% G163G (GGC→GGT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,8130GA15.8% 333.4 / 41.0 133G163G (GGC→GGTompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (59/52);  new base A (12/9);  total (72/61)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 136 total aligned reads displayed.

ACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAG  >  Exported/1439745‑1439882
                                                                    |                                                                     
aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc                                                                     >  1:2776364/1‑71 (MQ=255)
      cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc                                                               >  1:4028265/1‑71 (MQ=255)
      cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc                                                               >  1:901969/1‑71 (MQ=255)
         gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                             <  1:2827934/70‑1 (MQ=255)
               ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                >  1:2616335/1‑61 (MQ=255)
                 ttCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag                                                     >  1:3829570/1‑70 (MQ=255)
                  tCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGCCCGTCAACCAGACCgaag                                                     >  1:735838/1‑69 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                >  1:2568228/1‑57 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                >  1:4472787/1‑57 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                >  1:5603419/1‑57 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                                  >  1:4820904/1‑71 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                                  >  1:4258669/1‑71 (MQ=255)
                   cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                                  >  1:1775763/1‑71 (MQ=255)
                    aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                                  <  1:3112795/70‑1 (MQ=255)
                    aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                 <  1:5050667/71‑1 (MQ=255)
                    aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                 <  1:4362760/71‑1 (MQ=255)
                     ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                 >  1:4219703/1‑70 (MQ=255)
                        gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc                                                               >  1:1593116/1‑53 (MQ=255)
                        gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag                                                     <  1:2264004/63‑1 (MQ=255)
                        gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag                                                     <  1:4076419/63‑1 (MQ=255)
                        gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt                                             >  1:346052/1‑71 (MQ=255)
                        gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt                                             >  1:829005/1‑71 (MQ=255)
                         aTGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc                                                                  <  1:3463405/49‑1 (MQ=255)
                          tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt                                           <  1:594962/71‑1 (MQ=255)
                              ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa                                               <  1:5095300/63‑1 (MQ=255)
                              ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa                                               <  1:2691678/63‑1 (MQ=255)
                                ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAgt                                             <  1:1222257/61‑1 (MQ=2)
                                ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga                                      >  1:1921524/3‑69 (MQ=255)
                                ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga                                      >  1:245171/3‑69 (MQ=255)
                                gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                      <  1:2365396/70‑1 (MQ=255)
                                 tcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat                                     >  1:5514461/2‑68 (MQ=2)
                                 ccGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                      <  1:3448231/53‑1 (MQ=255)
                                   gttgttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATaa                                   <  1:913249/70‑2 (MQ=255)
                                   gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg                                  >  1:4051572/1‑71 (MQ=255)
                                    tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                >  1:4609455/1‑40 (MQ=255)
                                    tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg                                                        <  1:1208302/48‑1 (MQ=255)
                                    tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt                                 >  1:3365252/1‑71 (MQ=255)
                                    ttattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt                                 >  1:5490431/4‑71 (MQ=255)
                                     tattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGtt                                >  1:2472725/3‑70 (MQ=255)
                                      tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                             >  1:5206816/1‑41 (MQ=255)
                                      tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                             >  1:2124127/1‑41 (MQ=255)
                                      tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg                               >  1:2293748/1‑71 (MQ=255)
                                      gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg                               >  1:1540319/2‑71 (MQ=255)
                                      gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc                                <  1:4359271/69‑1 (MQ=255)
                                       ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc                              <  1:5324948/71‑1 (MQ=9)
                                       ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc                              <  1:3486189/71‑1 (MQ=9)
                                       ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                      <  1:4326007/47‑1 (MQ=255)
                                       ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                      <  1:755752/47‑1 (MQ=255)
                                        tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc                              >  1:1925733/1‑70 (MQ=9)
                                        tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc                              >  1:1432098/1‑70 (MQ=9)
                                         aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg                             >  1:77650/1‑70 (MQ=255)
                                         aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg                             >  1:2255134/1‑70 (MQ=255)
                                         aCCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa                            >  1:4607544/1‑69 (MQ=9)
                                          cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGCAGAAGTCAGTGTTACGGTAGGTCGCGaa                           <  1:4488359/71‑1 (MQ=255)
                                          cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGtt                                          <  1:1886572/56‑1 (MQ=255)
                                           ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg                          <  1:1428588/71‑1 (MQ=255)
                                             tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc                         >  1:1718842/4‑70 (MQ=9)
                                             tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc                         >  1:4504168/4‑70 (MQ=9)
                                              ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                                         >  1:2497059/1‑53 (MQ=255)
                                              gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAGTCAGTATTACGATAAGTCGCGACGcca                       >  1:3367110/3‑70 (MQ=9)
                                              gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca                       >  1:5310415/3‑70 (MQ=9)
                                               gTACTGAACAGCAAAGTTCAGGCCTTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                      <  1:2621710/71‑1 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                        <  1:4409602/69‑1 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                        <  1:1149380/69‑1 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg                       <  1:4788670/70‑1 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                      <  1:798438/71‑1 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                      >  1:2312503/1‑71 (MQ=255)
                                               gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                      >  1:3983863/1‑71 (MQ=255)
                                                tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCGGTGTTACGGTAGGTCGCGAAGc                         <  1:5030844/67‑1 (MQ=255)
                                                tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                     <  1:5506495/71‑1 (MQ=255)
                                                tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                     <  1:2037482/71‑1 (MQ=255)
                                                 aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa                           >  1:5538641/1‑64 (MQ=255)
                                                  cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                      <  1:2206403/52‑1 (MQ=255)
                                                  cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                      <  1:4804220/52‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                      <  1:5617523/35‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                <  1:1269071/41‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                <  1:2209405/41‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                <  1:3644829/41‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg                                              <  1:1015214/43‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc                         <  1:4635692/64‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc                         <  1:5188134/64‑1 (MQ=255)
                                                   tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                     <  1:1523523/68‑1 (MQ=255)
                                                    gAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag                                                     <  1:3798414/35‑1 (MQ=255)
                                                    gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa                                               <  1:4521908/41‑1 (MQ=255)
                                                     aaCAGCAAAGTTCAGGCCGTGAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa                           >  1:4705293/1‑60 (MQ=255)
                                                     aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa                           >  1:4595357/1‑60 (MQ=255)
                                                     aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc                >  1:4472473/1‑71 (MQ=255)
                                                      aCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt                                             >  1:316611/1‑41 (MQ=255)
                                                       cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc              <  1:1538613/71‑1 (MQ=255)
                                                       aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                  >  1:3733531/2‑51 (MQ=255)
                                                       aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                  >  1:1010531/2‑51 (MQ=255)
                                                       aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                  >  1:3497126/2‑51 (MQ=255)
                                                       aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                  >  1:5216332/2‑51 (MQ=255)
                                                           aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct          >  1:3365846/1‑71 (MQ=255)
                                                             aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                      <  1:1747386/41‑1 (MQ=255)
                                                             aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                      <  1:1058974/41‑1 (MQ=255)
                                                             aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGACGTTACCACGctgctg         >  1:5523568/1‑70 (MQ=255)
                                                                 cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg     >  1:3495420/1‑70 (MQ=255)
                                                                 cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg     >  1:798367/1‑70 (MQ=255)
                                                                   ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg  <  1:5264623/71‑1 (MQ=255)
                                                                    |                                                                     
ACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAG  >  Exported/1439745‑1439882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: