Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,840 G→A 16.2% N154N (AAC→AAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,8400GA16.2% 356.5 / 46.7 142N154N (AAC→AATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (64/55);  new base A (15/8);  total (79/63)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

TGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCG  >  Exported/1439771‑1439909
                                                                     |                                                                     
tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt                                                                      <  1:594962/71‑1 (MQ=255)
  ggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                                                                    <  1:2939076/71‑1 (MQ=255)
      ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga                                                                 >  1:245171/3‑69 (MQ=255)
      ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga                                                                 >  1:1921524/3‑69 (MQ=255)
      gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                                                 <  1:2365396/70‑1 (MQ=255)
         gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg                                                             >  1:3481802/1‑71 (MQ=255)
          tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg                                                             >  1:3235629/1‑70 (MQ=255)
          tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt                                                            <  1:5418776/71‑1 (MQ=255)
          ttgttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGt                                                            <  1:4163262/68‑1 (MQ=9)
          ttattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt                                                            >  1:5490431/4‑71 (MQ=255)
           tattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGtt                                                           >  1:2472725/3‑70 (MQ=255)
            tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg                                                          >  1:2293748/1‑71 (MQ=255)
            gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg                                                          >  1:2122834/2‑71 (MQ=255)
            gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg                                                          >  1:1540319/2‑71 (MQ=255)
            gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc                                                           <  1:4359271/69‑1 (MQ=255)
            gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc                                                           <  1:1903393/69‑1 (MQ=255)
             ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc                                                         <  1:399180/71‑1 (MQ=9)
               aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg                                                        >  1:2255134/1‑70 (MQ=255)
               aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg                                                        >  1:77650/1‑70 (MQ=255)
               aCCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa                                                       >  1:4607544/1‑69 (MQ=9)
                cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGCAGAAGTCAGTGTTACGGTAGGTCGCGaa                                                      <  1:4488359/71‑1 (MQ=255)
                 ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg                                                     <  1:1428588/71‑1 (MQ=255)
                   tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc                                                    >  1:1718842/4‑70 (MQ=9)
                    ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                                                                    >  1:2497059/1‑53 (MQ=255)
                    gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAGTCAGTATTACGATAAGTCGCGACGcca                                                  >  1:3367110/3‑70 (MQ=9)
                    gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca                                                  >  1:5310415/3‑70 (MQ=9)
                     gTACTGAACAGCAAAGTTCAGGCCTTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                                 <  1:2621710/71‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                                                   <  1:888923/69‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                                                   <  1:1149380/69‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                                                   <  1:4409602/69‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg                                                  <  1:4788670/70‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                                 >  1:3983863/1‑71 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                                 <  1:4839570/71‑1 (MQ=255)
                     gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                                 >  1:2312503/1‑71 (MQ=255)
                      tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCGGTGTTACGGTAGGTCGCGAAGc                                                    <  1:5030844/67‑1 (MQ=255)
                      tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                                                <  1:5506495/71‑1 (MQ=255)
                       aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa                                                      >  1:5538641/1‑64 (MQ=255)
                        cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                                                 <  1:4804220/52‑1 (MQ=255)
                         tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc                                                    <  1:4635692/64‑1 (MQ=255)
                         tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                                                <  1:1523523/68‑1 (MQ=255)
                           aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc                                           >  1:4472473/1‑71 (MQ=255)
                             cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc                                         <  1:5579924/71‑1 (MQ=255)
                             aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                                             >  1:72759/2‑51 (MQ=255)
                             aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                                             >  1:1010531/2‑51 (MQ=255)
                             aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                                             >  1:3733531/2‑51 (MQ=255)
                             aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                                             >  1:3497126/2‑51 (MQ=255)
                             aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg                                                             >  1:5216332/2‑51 (MQ=255)
                               gCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                                 >  1:4543191/1‑61 (MQ=255)
                                 aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct                                     >  1:3365846/1‑71 (MQ=255)
                                   aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                                                 <  1:1747386/41‑1 (MQ=255)
                                   aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                                                 <  1:1058974/41‑1 (MQ=255)
                                   aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGACGTTACCACGctgctg                                    >  1:5523568/1‑70 (MQ=255)
                                       cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg                                >  1:3495420/1‑70 (MQ=255)
                                       cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg                                >  1:5108051/1‑70 (MQ=255)
                                       cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg                                >  1:798367/1‑70 (MQ=255)
                                         ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg                             <  1:5264623/71‑1 (MQ=255)
                                         ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg                             <  1:792965/71‑1 (MQ=255)
                                           ccGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGt                            >  1:3921721/1‑70 (MQ=255)
                                            cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg                          <  1:5125337/71‑1 (MQ=255)
                                            cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg                          <  1:1414085/71‑1 (MQ=255)
                                            cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg                          >  1:625381/1‑71 (MQ=255)
                                              tCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGaa                              <  1:5215595/65‑1 (MQ=255)
                                              tCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt                         >  1:1187373/1‑70 (MQ=255)
                                                aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt                         >  1:5082359/1‑68 (MQ=255)
                                                aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt                         >  1:1870619/1‑68 (MQ=255)
                                                aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCa                       <  1:2939990/70‑1 (MQ=255)
                                                 aCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGa                     <  1:4514527/71‑1 (MQ=255)
                                                 aCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGa                     <  1:2192932/71‑1 (MQ=255)
                                                  ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGaa                    >  1:3472507/1‑71 (MQ=255)
                                                    aGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa                                  >  1:2420254/1‑55 (MQ=255)
                                                    aGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa                                  >  1:3307747/1‑55 (MQ=255)
                                                    aGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAAcc                  <  1:3328028/71‑1 (MQ=255)
                                                    aGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGATGCa                                  >  1:5355164/1‑55 (MQ=255)
                                                    aGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca                                                  >  1:1970352/1‑38 (MQ=16)
                                                     gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg                                                  <  1:1953240/38‑1 (MQ=255)
                                                     gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg                                                  <  1:1005961/38‑1 (MQ=255)
                                                     gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAg                      <  1:937581/66‑1 (MQ=255)
                                                     gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAg                      <  1:941465/66‑1 (MQ=255)
                                                     gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAg                      <  1:2365801/66‑1 (MQ=255)
                                                      aCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt                <  1:849701/71‑1 (MQ=255)
                                                      aCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt                <  1:3823201/71‑1 (MQ=255)
                                                      aCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt                <  1:1543241/71‑1 (MQ=255)
                                                        caaagaagCCATTGTTACGGTAGGTTGCAACACCATTAGCACGACCGTTCATGAAGTTGTc                        >  1:4247968/3‑61 (MQ=255)
                                                        caaagaagCCATTGTTACGGTAGGTTGCAACACCATTAGCACGACCGTTCATGAAGTTGTc                        >  1:507267/3‑61 (MQ=255)
                                                        cGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTa               <  1:2526094/70‑1 (MQ=255)
                                                          aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt            <  1:4895985/71‑1 (MQ=255)
                                                          aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt            >  1:4287459/1‑71 (MQ=255)
                                                            gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt          <  1:3775624/71‑1 (MQ=255)
                                                            gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt          <  1:227010/71‑1 (MQ=255)
                                                            gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtg           >  1:3868254/1‑70 (MQ=255)
                                                             aagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa                                            >  1:2246723/1‑36 (MQ=255)
                                                             aagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAgg             >  1:1728379/1‑67 (MQ=255)
                                                              agTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca        <  1:1767335/71‑1 (MQ=255)
                                                               gTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg                             <  1:1603331/49‑1 (MQ=255)
                                                                tCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcac       >  1:3225003/1‑70 (MQ=255)
                                                                  aGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac    >  1:4193063/1‑71 (MQ=255)
                                                                  aGTGTTACGGTAGGTCGCGAAGCAGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac    >  1:1523839/1‑71 (MQ=255)
                                                                    tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt                <  1:167129/57‑1 (MQ=255)
                                                                    tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac    >  1:5532442/1‑69 (MQ=255)
                                                                     gtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCg  <  1:1748050/70‑1 (MQ=255)
                                                                     |                                                                     
TGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCG  >  Exported/1439771‑1439909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: