| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,933 | G→T | 8.0% | S123S (TCC→TCA) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,933 | 0 | G | T | 8.0% | 472.8 / 14.9 | 148 | S123S (TCC→TCA) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (58/78); new base T (6/6); total (64/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
TACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTT > Exported/1439863‑1439998 | tACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAg > 1:2367988/1‑71 (MQ=255) aCCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAg > 1:404504/1‑70 (MQ=255) aCCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAg < 1:2073974/70‑1 (MQ=255) ccACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAgg < 1:1579249/70‑1 (MQ=255) ccACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa < 1:2856428/71‑1 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:4980864/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:2699539/71‑1 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:1523978/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:568697/1‑71 (MQ=255) gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg > 1:455742/1‑71 (MQ=255) gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg > 1:802831/1‑71 (MQ=255) ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:2587952/69‑1 (MQ=255) gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt > 1:2224268/1‑69 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:5096470/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:3702901/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:72602/71‑1 (MQ=255) aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa > 1:2516699/1‑70 (MQ=255) aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3136282/71‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5087546/57‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5234026/57‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:1776519/57‑1 (MQ=255) tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:1679774/1‑64 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAg > 1:1571097/1‑49 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg > 1:534128/1‑70 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5586634/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:77581/1‑71 (MQ=255) cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAg < 1:1201082/47‑1 (MQ=255) cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGaa > 1:2320880/1‑50 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:4179201/71‑1 (MQ=255) gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt < 1:4013174/55‑1 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:2129100/48‑1 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5497187/48‑1 (MQ=255) ccGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt > 1:3274189/1‑70 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:5600500/70‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg < 1:1385996/71‑1 (MQ=255) ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:2282188/1‑51 (MQ=255) gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg > 1:1314500/1‑70 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1029332/2‑71 (MQ=2) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:4869008/39‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:2815381/38‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:2677133/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:1387875/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4796874/47‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc > 1:2317450/1‑60 (MQ=255) caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc > 1:3933879/1‑70 (MQ=2) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:541706/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:594004/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:2054869/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:4747159/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:1125920/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc > 1:5221540/1‑63 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:1899483/71‑1 (MQ=255) accGAATTATGGCAGTACGTCGGTACAGGAAGTTACGTCATAaacaa > 1:3301902/1‑47 (MQ=255) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3858876/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3865498/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:2183233/71‑1 (MQ=2) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:3431447/41‑1 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:5397797/44‑1 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4828836/44‑1 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1343961/44‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1031628/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3275574/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:2420399/43‑1 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa > 1:4436782/1‑41 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:3116911/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:2565344/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:1664598/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4875436/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:1224023/1‑68 (MQ=255) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:1639409/67‑1 (MQ=9) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAg > 1:4231383/2‑71 (MQ=2) aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca > 1:5266472/1‑42 (MQ=255) ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:5393229/39‑1 (MQ=255) ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3525080/39‑1 (MQ=255) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:2916126/60‑1 (MQ=2) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4153494/37‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3709835/37‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa > 1:357278/1‑46 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:1653329/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa < 1:4663147/58‑1 (MQ=255) cTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa > 1:3960016/1‑65 (MQ=2) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:5207718/42‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa < 1:4038902/61‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:5182644/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:379425/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:748351/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:379437/57‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAACCAACGCCGTAGTTa > 1:2268116/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:3183425/40‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:5438572/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:3098874/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:1853459/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg > 1:855759/1‑71 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:4490468/70‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1064637/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:5367911/68‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt < 1:4948393/71‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3516121/70‑1 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg > 1:4526654/1‑49 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:2205639/1‑68 (MQ=255) | TACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTT > Exported/1439863‑1439998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |