Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,440,224 G→A 23.9% N26N (AAC→AAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,440,2240GA23.9% 205.6 / 51.5 96N26N (AAC→AATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (44/29);  new base A (16/7);  total (60/36)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTT  >  Exported/1440154‑1440292
                                                                      |                                                                    
cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCTTCTttg                                                                      <  1:180053/71‑1 (MQ=255)
cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg                                                                      <  1:3816773/71‑1 (MQ=255)
cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg                                                                      <  1:696502/71‑1 (MQ=255)
      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                 >  1:2978238/1‑70 (MQ=255)
      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                 <  1:4519547/70‑1 (MQ=255)
       gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa                                                                <  1:3901941/70‑1 (MQ=255)
        ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATaa                                                                >  1:4584766/4‑69 (MQ=2)
        ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc                                                              >  1:5075339/4‑71 (MQ=9)
         tgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt                                                                    >  1:5530487/1‑64 (MQ=255)
           tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                            <  1:2968300/70‑1 (MQ=255)
           tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                            >  1:323894/1‑70 (MQ=255)
             agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag                                                         >  1:2106970/1‑71 (MQ=255)
             agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag                                                         >  1:4428153/1‑71 (MQ=255)
                gaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagcag                                                      >  1:1661661/1‑71 (MQ=255)
                aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcggctg                                                      >  1:1430497/2‑69 (MQ=9)
                 aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc                                                           >  1:3737092/1‑65 (MQ=255)
                     aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                 >  1:1521748/1‑71 (MQ=255)
                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca                                                          >  1:4284700/1‑62 (MQ=255)
                      gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa                                                               >  1:2672177/1‑56 (MQ=255)
                      gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                <  1:3563717/71‑1 (MQ=255)
                         cAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca                                                          <  1:4235278/58‑1 (MQ=255)
                          aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                            <  1:482148/71‑1 (MQ=255)
                           ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg                                           <  1:767531/71‑1 (MQ=255)
                           ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca                                           <  1:2463377/71‑2 (MQ=12)
                                tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg                                       <  1:3581500/70‑1 (MQ=255)
                                  tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc                                      <  1:3016962/69‑1 (MQ=2)
                                  tACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt                                                                    >  1:605422/1‑39 (MQ=255)
                                  tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca                                                          >  1:5107266/1‑49 (MQ=255)
                                  tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc                                                           >  1:2609717/1‑48 (MQ=255)
                                  tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                 <  1:3279653/58‑1 (MQ=255)
                                  tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                    >  1:1528568/1‑71 (MQ=255)
                                  aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc                                                              >  1:1861550/2‑45 (MQ=17)
                                  aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc                                                              >  1:5362326/2‑45 (MQ=17)
                                  aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc                                                              >  1:4446776/2‑45 (MQ=17)
                                  aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc                                                              >  1:1064332/2‑45 (MQ=17)
                                  aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcg                                                          >  1:2065895/2‑48 (MQ=17)
                                   aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                         >  1:470653/1‑65 (MQ=2)
                                   aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                         >  1:165270/1‑65 (MQ=2)
                                   aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                         >  1:1285980/1‑65 (MQ=2)
                                    cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                    <  1:4598928/69‑1 (MQ=255)
                                     tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc                                          <  1:4836368/62‑1 (MQ=255)
                                     tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc                                          <  1:934179/62‑1 (MQ=255)
                                     tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc                                   >  1:4481721/1‑69 (MQ=255)
                                       tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:4389517/1‑70 (MQ=255)
                                       tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:1937160/1‑70 (MQ=255)
                                       tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:496206/1‑70 (MQ=255)
                                       tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:520484/1‑70 (MQ=255)
                                       tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:1621446/1‑70 (MQ=255)
                                         ccatACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag                             <  1:3618084/68‑1 (MQ=255)
                                            tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagca                                                       <  1:1616235/42‑1 (MQ=255)
                                            tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagca                                                       <  1:5490912/42‑1 (MQ=255)
                                            tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt                                                   >  1:4747511/1‑46 (MQ=255)
                                            tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                              <  1:4509361/51‑1 (MQ=255)
                                            tACAGATCTAATTTGTTCCGTCTTTGTTGTAAACTTcagca                                                       <  1:4550452/41‑1 (MQ=39)
                                             aCAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                >  1:2338551/1‑64 (MQ=255)
                                             aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcggc                                   <  1:1619161/61‑4 (MQ=255)
                                             aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag                             <  1:235588/67‑1 (MQ=255)
                                              cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                            >  1:3135967/1‑35 (MQ=255)
                                              cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc                                                           >  1:5527792/1‑36 (MQ=255)
                                              cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc                                                           >  1:172728/1‑36 (MQ=255)
                                              cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc                                                           >  1:4225050/1‑36 (MQ=255)
                                              cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg                        <  1:1884941/71‑1 (MQ=255)
                                               aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                 <  1:3575862/45‑1 (MQ=255)
                                               aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                       >  1:4972598/1‑71 (MQ=255)
                                               aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                       >  1:1228954/1‑71 (MQ=255)
                                                gATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagc                                            <  1:5338854/49‑1 (MQ=16)
                                                 aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCTTTtgc                                               >  1:4884715/1‑45 (MQ=255)
                                                 aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                               >  1:1788181/1‑45 (MQ=255)
                                                  tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGtt                                                  >  1:4738667/1‑41 (MQ=255)
                                                   cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat                    >  1:2648995/1‑67 (MQ=255)
                                                   cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat                    >  1:4935129/1‑67 (MQ=255)
                                                    tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt                                                   >  1:439235/1‑38 (MQ=255)
                                                    tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt                                                   >  1:1495279/1‑38 (MQ=255)
                                                    tAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCt                                        <  1:1763009/49‑1 (MQ=12)
                                                     aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                 >  1:2153372/1‑71 (MQ=255)
                                                      aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                    <  1:788333/67‑1 (MQ=255)
                                                      aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagcac                                          >  1:1627459/1‑43 (MQ=9)
                                                      aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca                                           >  1:2573411/1‑43 (MQ=9)
                                                       tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc                   <  1:5335710/67‑1 (MQ=255)
                                                       tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga               <  1:204270/71‑1 (MQ=255)
                                                       tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                 >  1:2103598/1‑69 (MQ=255)
                                                         tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                >  1:2438350/1‑36 (MQ=255)
                                                         tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg             >  1:1721441/1‑71 (MQ=255)
                                                          gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg             <  1:3760441/70‑1 (MQ=255)
                                                              ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg        <  1:75376/71‑1 (MQ=255)
                                                                gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa      >  1:3093427/1‑71 (MQ=255)
                                                                 tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGCGCTGGGACCAGGAGGGACAGTAc     >  1:2184546/1‑71 (MQ=255)
                                                                 tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa                     <  1:2971903/55‑1 (MQ=255)
                                                                 tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc                   <  1:3814144/57‑1 (MQ=255)
                                                                 tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc     >  1:1652277/1‑71 (MQ=255)
                                                                  cTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                    <  1:4306071/39‑1 (MQ=255)
                                                                   tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                 <  1:4770638/57‑1 (MQ=255)
                                                                   tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt   >  1:5492746/1‑71 (MQ=255)
                                                                   tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt   >  1:2461263/1‑71 (MQ=255)
                                                                   tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt   >  1:4708676/1‑71 (MQ=255)
                                                                   tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt   >  1:3535897/1‑71 (MQ=255)
                                                                     tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGATCTGGGACCAGGAGGGACAGTACttt  <  1:1410667/70‑1 (MQ=255)
                                                                     tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt  >  1:4949594/1‑70 (MQ=255)
                                                                     tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt  >  1:3792894/1‑70 (MQ=255)
                                                                     tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt   <  1:3888863/69‑1 (MQ=255)
                                                                      |                                                                    
CATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTT  >  Exported/1440154‑1440292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: