Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,440,244 T→G 11.5% N20H (AAC→CAC) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,440,2440TG11.5% 196.5 / 21.7 87N20H (AAC→CAC) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base T (47/30);  new base G (4/6);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

AGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCC  >  Exported/1440175‑1440312
                                                                     |                                                                    
aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                                     >  1:1521748/1‑71 (MQ=255)
 gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                                    <  1:3563717/71‑1 (MQ=255)
     aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                                                <  1:482148/71‑1 (MQ=255)
      ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg                                                               <  1:767531/71‑1 (MQ=255)
      ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca                                                               <  1:2463377/71‑2 (MQ=12)
           tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg                                                           <  1:3581500/70‑1 (MQ=255)
             tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc                                                          <  1:3016962/69‑1 (MQ=2)
             tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                                     <  1:3279653/58‑1 (MQ=255)
             tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                        >  1:1528568/1‑71 (MQ=255)
              aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                                             >  1:2294653/1‑65 (MQ=2)
              aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                                             >  1:1285980/1‑65 (MQ=2)
              aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                                             >  1:470653/1‑65 (MQ=2)
              aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc                                                             >  1:165270/1‑65 (MQ=2)
               cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                        <  1:4598928/69‑1 (MQ=255)
                tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc                                                              <  1:934179/62‑1 (MQ=255)
                tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc                                                              <  1:4836368/62‑1 (MQ=255)
                tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc                                                       >  1:4481721/1‑69 (MQ=255)
                  tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:520484/1‑70 (MQ=255)
                  tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:496206/1‑70 (MQ=255)
                  tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:1937160/1‑70 (MQ=255)
                  tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:4389517/1‑70 (MQ=255)
                  tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:1621446/1‑70 (MQ=255)
                    ccatACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag                                                 <  1:3618084/68‑1 (MQ=255)
                       tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                                                  <  1:4509361/51‑1 (MQ=255)
                        aCAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                    >  1:2338551/1‑64 (MQ=255)
                        aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcggc                                                       <  1:1619161/61‑4 (MQ=255)
                        aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag                                                 <  1:235588/67‑1 (MQ=255)
                         cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg                                            <  1:1884941/71‑1 (MQ=255)
                          aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                                     <  1:3575862/45‑1 (MQ=255)
                          aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                                           >  1:1228954/1‑71 (MQ=255)
                          aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                                           >  1:4972598/1‑71 (MQ=255)
                           gATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagc                                                                <  1:5338854/49‑1 (MQ=16)
                            aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCTTTtgc                                                                   >  1:4884715/1‑45 (MQ=255)
                            aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                                   >  1:1788181/1‑45 (MQ=255)
                             tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGtt                                                                      >  1:4738667/1‑41 (MQ=255)
                              cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat                                        >  1:2648995/1‑67 (MQ=255)
                              cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat                                        >  1:4935129/1‑67 (MQ=255)
                               tAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCt                                                            <  1:1763009/49‑1 (MQ=12)
                                aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                                    >  1:5116743/1‑40 (MQ=255)
                                aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                     >  1:2153372/1‑71 (MQ=255)
                                 aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                                        <  1:788333/67‑1 (MQ=255)
                                 aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagcac                                                              >  1:1627459/1‑43 (MQ=9)
                                 aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca                                                               >  1:2573411/1‑43 (MQ=9)
                                  tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc                                       <  1:5335710/67‑1 (MQ=255)
                                  tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                                   <  1:3937048/71‑1 (MQ=255)
                                  tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                                   <  1:204270/71‑1 (MQ=255)
                                  tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                     >  1:2103598/1‑69 (MQ=255)
                                    tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                                    >  1:2438350/1‑36 (MQ=255)
                                    tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg                                 >  1:1721441/1‑71 (MQ=255)
                                     gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg                                 <  1:988937/70‑1 (MQ=255)
                                     gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg                                 <  1:3760441/70‑1 (MQ=255)
                                         ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg                            <  1:75376/71‑1 (MQ=255)
                                           gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa                          >  1:3093427/1‑71 (MQ=255)
                                            tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGCGCTGGGACCAGGAGGGACAGTAc                         >  1:2184546/1‑71 (MQ=255)
                                            tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa                                         <  1:2971903/55‑1 (MQ=255)
                                            tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc                                       <  1:3814144/57‑1 (MQ=255)
                                            tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc                         >  1:1652277/1‑71 (MQ=255)
                                             cTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                        <  1:4306071/39‑1 (MQ=255)
                                              tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                     <  1:4770638/57‑1 (MQ=255)
                                              tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                       >  1:3535897/1‑71 (MQ=255)
                                              tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                       >  1:5492746/1‑71 (MQ=255)
                                              tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                       >  1:2461263/1‑71 (MQ=255)
                                              tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                       >  1:4708676/1‑71 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGATCTGGGACCAGGAGGGACAGTACttt                      <  1:1410667/70‑1 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                      >  1:3792894/1‑70 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                      <  1:169379/70‑1 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                      >  1:3094092/1‑70 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                      >  1:4949594/1‑70 (MQ=255)
                                                tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                       <  1:3888863/69‑1 (MQ=255)
                                                   tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca                                                     >  1:3527272/1‑36 (MQ=255)
                                                   tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca                                                     >  1:4931922/1‑36 (MQ=255)
                                                   tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca                                                     >  1:1156736/1‑36 (MQ=255)
                                                    gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc                         >  1:4123593/1‑63 (MQ=255)
                                                    gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc                         >  1:3194175/1‑63 (MQ=255)
                                                    gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt                 >  1:1963810/1‑71 (MQ=255)
                                                     tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt                >  1:3661286/1‑71 (MQ=255)
                                                     tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt                <  1:314447/71‑1 (MQ=255)
                                                     tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt                 <  1:649078/70‑1 (MQ=255)
                                                      aaaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa                                         >  1:1004588/1‑45 (MQ=255)
                                                       aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGc                                              >  1:5261197/1‑39 (MQ=255)
                                                       aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc               <  1:2271148/70‑1 (MQ=255)
                                                           tCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt           >  1:3377800/1‑70 (MQ=255)
                                                            cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta         >  1:772416/1‑71 (MQ=255)
                                                            cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt           <  1:3587214/69‑1 (MQ=255)
                                                            cagcagCGTTTGATGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta         >  1:3994858/1‑71 (MQ=255)
                                                             agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat        <  1:375657/71‑1 (MQ=255)
                                                             agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt           <  1:2394222/68‑1 (MQ=255)
                                                                agcGTTTGCTGCGACTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTaa     >  1:1496321/1‑71 (MQ=255)
                                                                 gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAc    >  1:296204/1‑71 (MQ=255)
                                                                 gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACATTAACTTTCATGTTATTAAc    >  1:115510/1‑71 (MQ=255)
                                                                   gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAccc  >  1:1424613/1‑71 (MQ=255)
                                                                     |                                                                    
AGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCC  >  Exported/1440175‑1440312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: