Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 309,825 T→C 29.7% V1016V (GTA→GTG) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported309,8250TC29.7% 118.9 / 60.1 85V1016V (GTA→GTG) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (24/35);  new base C (11/14);  total (35/50)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

TTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAG  >  Exported/309759‑309895
                                                                  |                                                                      
ttCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg                                                                    >  1:3118460/1‑71 (MQ=255)
 tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt                                                                   >  1:3062001/1‑71 (MQ=255)
  cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                  >  1:726645/1‑71 (MQ=255)
   aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                  <  1:5632875/70‑1 (MQ=255)
   aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                  <  1:5639346/70‑1 (MQ=255)
         gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt                                                             <  1:4802089/69‑1 (MQ=255)
         gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt                                                             <  1:1037312/69‑1 (MQ=255)
             ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                       >  1:1446701/2‑71 (MQ=16)
             ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                       >  1:221571/2‑71 (MQ=16)
             cTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg                                                                    <  1:5569396/58‑1 (MQ=255)
              tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg                                                                    <  1:3829365/57‑1 (MQ=255)
              tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg                                                                 <  1:1550459/60‑1 (MQ=255)
              tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                       <  1:701302/70‑1 (MQ=255)
              tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                       <  1:1254904/70‑1 (MQ=255)
              tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                       <  1:2154671/70‑1 (MQ=255)
               aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc                                                     >  1:3255651/1‑71 (MQ=255)
                aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                      <  1:3234260/69‑1 (MQ=255)
                aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                      <  1:3879147/69‑1 (MQ=255)
                aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                      <  1:2545637/69‑1 (MQ=255)
                 aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg                                                   >  1:3422099/1‑71 (MQ=255)
                   cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTCCAGTACCGTTGCGGTCACCATcc                                                     >  1:4777737/1‑66 (MQ=255)
                    ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                 <  1:3265306/70‑1 (MQ=255)
                      cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc                                                                <  1:4354259/53‑1 (MQ=255)
                      cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc                                                                <  1:2851986/53‑1 (MQ=255)
                      cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc                                                                <  1:3891948/53‑1 (MQ=255)
                        gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAGCg                                                                    <  1:1792654/47‑1 (MQ=255)
                         cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                            <  1:4164197/70‑1 (MQ=255)
                           aaCCACCACAAAGAATACGGAACGAAGAAGATTGCCAGTAc                                                                      >  1:3598405/1‑41 (MQ=38)
                            accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc                                        >  1:4442604/1‑71 (MQ=255)
                             tcaccacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTAcccc                                        >  1:314986/2‑70 (MQ=255)
                             ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                               >  1:5409611/1‑63 (MQ=255)
                               accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                 <  1:3281504/59‑1 (MQ=255)
                               accacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTaccc                                         >  1:5354213/1‑65 (MQ=16)
                                 cacaAAGAATACCGGAACGAAGAAGATTGCCAGTAc                                                                      >  1:532980/1‑36 (MQ=255)
                                 cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                 <  1:1440765/57‑1 (MQ=255)
                                 cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                               >  1:4718558/1‑59 (MQ=255)
                                 cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                   >  1:4126667/1‑71 (MQ=255)
                                  acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc                                  >  1:5554540/1‑71 (MQ=255)
                                   caAAGAATACCGGAACTAAGAAGATTGCCAGTACCGTTGCGGTCAc                                                          <  1:1065477/46‑1 (MQ=255)
                                   caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTTCGGTCAc                                                          <  1:4336929/46‑1 (MQ=255)
                                   caAAGAATACCGGAACCAAGAAGATTGCCAGGACCGTTGCGGCCAc                                                          <  1:5163425/46‑1 (MQ=255)
                                     aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                >  1:5162098/1‑70 (MQ=255)
                                     aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                >  1:849491/1‑68 (MQ=12)
                                      aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                  <  1:625993/67‑1 (MQ=255)
                                      aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                  <  1:182003/67‑1 (MQ=255)
                                      aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                  <  1:847248/67‑1 (MQ=255)
                                      aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                 <  1:973145/52‑1 (MQ=12)
                                      aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                 <  1:31127/52‑1 (MQ=12)
                                       gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag                             >  1:2923753/1‑69 (MQ=255)
                                         acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                           >  1:3298615/3‑71 (MQ=255)
                                         acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                           >  1:4712527/3‑71 (MQ=255)
                                          tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                >  1:2475731/1‑65 (MQ=255)
                                          tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                >  1:5670355/1‑65 (MQ=255)
                                           aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         <  1:5275557/71‑1 (MQ=255)
                                            ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                           <  1:878289/52‑1 (MQ=255)
                                             cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                >  1:5372972/1‑60 (MQ=255)
                                              ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                           <  1:5521459/50‑1 (MQ=255)
                                              ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                      >  1:309971/1‑71 (MQ=255)
                                              ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                      <  1:2365160/71‑1 (MQ=255)
                                               gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                     <  1:2736173/69‑1 (MQ=255)
                                               gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                     <  1:2910880/69‑1 (MQ=255)
                                               gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                     <  1:2729606/71‑1 (MQ=255)
                                                aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                         >  1:3542944/1‑50 (MQ=255)
                                                aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                         >  1:5452146/1‑50 (MQ=255)
                                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                   >  1:2623255/1‑71 (MQ=255)
                                                  cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                  >  1:2352911/1‑71 (MQ=255)
                                                  cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         >  1:1336105/1‑64 (MQ=255)
                                                  cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         >  1:487488/1‑64 (MQ=255)
                                                   gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                              <  1:5150395/42‑1 (MQ=255)
                                                   gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                 <  1:4858816/71‑1 (MQ=255)
                                                   gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                 <  1:1760126/71‑1 (MQ=12)
                                                    aagaagATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                              <  1:3118294/41‑1 (MQ=255)
                                                    aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         <  1:2606199/62‑1 (MQ=255)
                                                    aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         <  1:1588986/62‑1 (MQ=255)
                                                    aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                         <  1:4603109/62‑1 (MQ=255)
                                                      gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg              <  1:1306413/71‑1 (MQ=255)
                                                      gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg              <  1:1517037/71‑1 (MQ=255)
                                                       aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa             <  1:448472/71‑1 (MQ=255)
                                                         gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc           <  1:91297/71‑1 (MQ=255)
                                                         gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc           >  1:3420077/1‑71 (MQ=255)
                                                         gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc           >  1:3216696/1‑71 (MQ=255)
                                                          aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc           <  1:2801117/70‑1 (MQ=255)
                                                          aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                  <  1:4069408/63‑1 (MQ=255)
                                                          aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                  <  1:4018313/63‑1 (MQ=255)
                                                             gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg        >  1:4999943/1‑70 (MQ=255)
                                                                  tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg  >  1:1582566/1‑71 (MQ=255)
                                                                  |                                                                      
TTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAG  >  Exported/309759‑309895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: