| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,552,249 | G→A | 100% | intergenic (+119/+65) | torI → / ← dsdC | response regulator inhibitor for tor operon/DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,552,249 | 0 | G | A | 77.8% | 130.9 / 34.3 | 72 | intergenic (+119/+65) | torI/dsdC | response regulator inhibitor for tor operon/DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/16); new base A (35/21); total (35/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
CCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAT > Exported/1552182‑1552310 | cccTGCTGCTTCAAGTAGATGTCTGCAGGTGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAAcc > 1:643968/1‑71 (MQ=255)cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAAcc > 1:3860894/1‑71 (MQ=255)cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAAc < 1:5077644/70‑1 (MQ=255) ctTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaatt < 1:2844457/70‑1 (MQ=255) ctTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaatt < 1:2560091/70‑1 (MQ=255) ctTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt < 1:94013/70‑1 (MQ=255) ctTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt < 1:353295/70‑1 (MQ=255) gTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg < 1:5457246/71‑1 (MQ=255) tAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg > 1:827243/1‑70 (MQ=255) gATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAGAACCGTAAATTAAATTCGAAc < 1:472317/71‑1 (MQ=255) tGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTa < 1:2041168/71‑1 (MQ=255) tGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTa < 1:2460505/71‑1 (MQ=255) tGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTa < 1:2453862/71‑1 (MQ=255) gTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaaa < 1:1544813/61‑1 (MQ=255) gTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAg > 1:1998871/1‑71 (MQ=255) tCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAg > 1:2193488/1‑70 (MQ=255) cAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCa < 1:4012569/68‑1 (MQ=255) cAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGta > 1:2805578/1‑49 (MQ=255) gACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAAt < 1:2139922/71‑1 (MQ=255) gACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGaa > 1:1044878/1‑70 (MQ=255) gACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt < 1:316000/71‑1 (MQ=255) gACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt < 1:4425711/71‑1 (MQ=255) acacCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCg < 1:1817864/41‑1 (MQ=255) acacCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATa < 1:2671832/71‑1 (MQ=255) aTCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaa > 1:3262800/1‑40 (MQ=255) aTCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaa > 1:2398290/1‑40 (MQ=255) aTCCTTGTGTTTATCCATAAAACCACATAAAAACCGTAAATTAAATTc > 1:3874734/1‑48 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaattaaa < 1:906970/44‑1 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaattaaa < 1:2356231/44‑1 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa > 1:2357453/1‑43 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa > 1:2364124/1‑43 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg < 1:2373984/61‑1 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGca > 1:4418292/1‑70 (MQ=255) tCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGca > 1:1920351/1‑70 (MQ=255) ccTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaa > 1:3081982/1‑38 (MQ=255) ccTTGTGTTTATCCCTAAAACCACATAAAAACCGtaa > 1:1365851/1‑37 (MQ=255) ccTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:4841250/1‑71 (MQ=255) ccTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:5025785/1‑71 (MQ=255) cTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCacac < 1:5690007/71‑1 (MQ=255) cTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCacac < 1:379662/71‑1 (MQ=255) cTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg < 1:594598/46‑1 (MQ=255) cTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg < 1:601524/46‑1 (MQ=255) cTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAg > 1:1458843/1‑52 (MQ=255) ttGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCg < 1:1044103/45‑1 (MQ=255) ttGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCacac > 1:844924/1‑70 (MQ=255) gtgtTTATCCCTAAAACCACATAAAAACCGTATATTAAATTCGAACTAGCAGGCCGAATAAGGCACAcc < 1:5334203/69‑1 (MQ=255) tgtTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAgg < 1:2774175/71‑1 (MQ=255) tgtTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGaa > 1:3178307/1‑44 (MQ=255) tgtTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGaa > 1:2020117/1‑44 (MQ=255) tttATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAgg < 1:3011098/69‑1 (MQ=255) tttATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg < 1:12381/53‑1 (MQ=255) cccTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt < 1:213020/51‑1 (MQ=255) ccTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGt < 1:4065981/70‑1 (MQ=255) ccTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa < 1:3647562/48‑1 (MQ=255) ccTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa < 1:5512894/48‑1 (MQ=255) cTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa < 1:2286845/47‑1 (MQ=255) tAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGGGGt > 1:402596/1‑71 (MQ=255) aaaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGcc > 1:1403328/1‑43 (MQ=255) aaaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCg > 1:4881652/1‑66 (MQ=255) aaaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCg > 1:1444597/1‑66 (MQ=255) aaaCCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGttt < 1:800798/71‑1 (MQ=255) aaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGca > 1:99067/1‑52 (MQ=255) aaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGttt > 1:2520404/1‑71 (MQ=255) aaaCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGt < 1:1519964/69‑1 (MQ=255) aCCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTa < 1:1535236/71‑1 (MQ=255) aCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTa > 1:5079179/1‑71 (MQ=255) aCCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTa > 1:1051355/1‑71 (MQ=255) ccACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAt > 1:3252346/1‑71 (MQ=255) acaTAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:5643809/1‑49 (MQ=255) acaTAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:1032110/1‑49 (MQ=255) acaTAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCacac > 1:1386247/1‑50 (MQ=255) acaTAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAAc > 1:3407548/1‑57 (MQ=255) aaaaACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:5564990/1‑45 (MQ=255) aaaaACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:5449094/1‑45 (MQ=255) aaaaACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca > 1:1333727/1‑45 (MQ=255) | CCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAT > Exported/1552182‑1552310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |