| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,591,605 | G→A | 33.1% | intergenic (+64/+125) | yfeS → / ← cysM | conserved hypothetical protein/cysteine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,591,605 | 0 | G | A | 33.1% | 158.6 / 120.8 | 145 | intergenic (+64/+125) | yfeS/cysM | conserved hypothetical protein/cysteine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (43/54); new base A (29/19); total (72/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 151 total aligned reads displayed.
AAATTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAG > Exported/1591535‑1591675 | aaaTTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATgcggcg > 1:2273374/1‑71 (MQ=255)aaaTTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATgcggcg > 1:1867160/1‑71 (MQ=255) ttAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa < 1:2699824/71‑1 (MQ=255) aTACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTTTTTATGCCGGATGCGGCGTAAACGc < 1:1482433/71‑1 (MQ=255) gCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:644159/1‑63 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATaaa < 1:3281216‑M1/15‑1 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCtt > 1:257903‑M1/48‑68 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTa > 1:284181‑M1/48‑69 (MQ=255) tttGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:5493286/58‑1 (MQ=255) ataggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGc > 1:1068605‑M1/46‑63 (MQ=255) tGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:5612222/1‑58 (MQ=255) aggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAAc < 1:1522535‑M1/16‑1 (MQ=255) aggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAAc < 1:5605964‑M1/16‑1 (MQ=255) aggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAAc < 1:4449442‑M1/16‑1 (MQ=255) ggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTAtccgacc < 1:5406054‑M1/29‑4 (MQ=255) ggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTAtccgacc < 1:4881947‑M1/29‑4 (MQ=255) ggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTAtccgacc < 1:3460856‑M1/29‑4 (MQ=255) gTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 1:2131209/70‑1 (MQ=255) cGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2060160/53‑1 (MQ=255) ggCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3732465/52‑1 (MQ=255) ggCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:4785641/71‑1 (MQ=255) ctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTa < 1:41480‑M1/31‑1 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTa < 1:2746008‑M1/31‑1 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc > 1:2185675‑M1/40‑71 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc < 1:2792159‑M1/32‑1 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc > 1:4427802‑M1/39‑70 (MQ=255) gataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACa < 1:2192035‑M1/33‑1 (MQ=255) gataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACa < 1:1148611‑M1/33‑1 (MQ=255) gataagacgcggcaagcgtctcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCg > 1:146277‑M1/38‑63 (MQ=255) gataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTa > 1:3476384‑M1/38‑50 (MQ=255) gataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa < 1:653082‑M1/33‑1 (MQ=255) ctgataagacggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa < 1:1112508‑M1/33‑1 (MQ=255) ataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaa < 1:4676571‑M1/34‑1 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACaa < 1:4663289‑M1/34‑1 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa > 1:3968541‑M1/37‑69 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa > 1:3133308‑M1/37‑69 (MQ=255) taagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTAc > 1:2505612‑M1/36‑67 (MQ=255) taagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTAc > 1:3689311‑M1/36‑67 (MQ=255) taagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa < 1:673634‑M1/36‑1 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa < 1:3316933‑M1/36‑1 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAt < 1:3698561‑M1/37‑1 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAt < 1:1652032‑M1/37‑1 (MQ=255) aagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt < 1:5355782‑M1/37‑1 (MQ=255) aagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt < 1:4347264‑M1/37‑1 (MQ=255) agacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATc > 1:3931994‑M1/34‑71 (MQ=255) agacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATc > 1:455065‑M1/34‑71 (MQ=255) agacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCg > 1:2936739‑M1/34‑59 (MQ=255) aacaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc > 1:4471248/1‑71 (MQ=255) aacaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc > 1:1993540/1‑71 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAtca > 1:1472609‑M1/33‑70 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAtca < 1:2919820‑M1/39‑2 (MQ=255) gacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAtca < 1:2232205‑M1/39‑2 (MQ=255) acaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa < 1:4865689/54‑1 (MQ=255) acTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:4041016/42‑1 (MQ=255) cTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:553422/1‑43 (MQ=255) cTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACg < 1:3071542/45‑1 (MQ=255) ttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa > 1:670278‑M1/27‑62 (MQ=255) ttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTaa > 1:4081563‑M1/27‑71 (MQ=255) gTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTaa > 1:3292707/1‑71 (MQ=255) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc > 1:235094/1‑67 (MQ=39) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAt > 1:4599416/1‑71 (MQ=255) caagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:209120‑M1/26‑70 (MQ=255) tAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAtt > 1:2591310/1‑71 (MQ=40) tAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAtt > 1:2474244/1‑71 (MQ=40) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:2446982‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:2920001‑M1/25‑69 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCtaaa < 1:2228188‑M1/46‑2 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACgctaaatc > 1:4238166‑M1/24‑68 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat < 1:2082957‑M1/47‑3 (MQ=255) gcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa > 1:4811518‑M1/23‑71 (MQ=255) aaaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTc < 1:1177903/70‑1 (MQ=39) aaaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCa < 1:3609408/71‑1 (MQ=38) aaaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCa < 1:2427778/71‑1 (MQ=38) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:187267‑M1/22‑70 (MQ=255) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:3585021‑M1/22‑70 (MQ=255) cgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:519117‑M1/50‑2 (MQ=255) gtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAAtcagg < 1:1299606‑M1/51‑3 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:3384337‑M1/20‑64 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:3391076‑M1/20‑64 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc > 1:5118810‑M1/20‑68 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcagga > 1:1080715‑M1/20‑68 (MQ=255) cgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:754138‑M1/19‑63 (MQ=255) gTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAatata < 1:3988206/71‑1 (MQ=39) gcgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATAtt > 1:1995596/1‑71 (MQ=39) gcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:3826488‑M1/11‑59 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTc < 1:2608126/56‑1 (MQ=35) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAc > 1:2781268/1‑69 (MQ=255) tATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaatca > 1:305977/1‑68 (MQ=11) tATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaatc < 1:4377490/70‑3 (MQ=11) gatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:4317859‑M1/50‑2 (MQ=255) gatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:228643‑M1/50‑2 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGc > 1:2859712/1‑71 (MQ=11) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCt < 1:321210/41‑1 (MQ=32) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAgg > 1:161687/1‑70 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc > 1:3033291/1‑71 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc < 1:487477/71‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGc > 1:1538705/1‑55 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCt < 1:4258487/71‑1 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTa > 1:4840718/1‑65 (MQ=11) gTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg > 1:1172367/1‑71 (MQ=255) | AAATTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAG > Exported/1591535‑1591675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |