| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,614,522 | A→G | 27.4% | intergenic (‑147/+70) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,614,522 | 0 | A | G | 27.4% | 100.3 / 39.0 | 62 | intergenic (‑147/+70) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base A (23/22); new base G (11/6); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
TGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAT > Exported/1614459‑1614591 | cggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCt > 1:3433141/2‑70 (MQ=14) ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa > 1:2150067/1‑71 (MQ=255) ggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCtt < 1:705783/70‑1 (MQ=14) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:4773168/1‑71 (MQ=255) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:1463460/1‑71 (MQ=255) aTAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc > 1:2344458/1‑71 (MQ=18) tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATcc > 1:4978755/1‑71 (MQ=255) aaaaCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCg > 1:1016883/4‑71 (MQ=18) aaaaCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCg > 1:5645550/4‑71 (MQ=18) gACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc > 1:4877157/1‑70 (MQ=255) cgcgACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGccc > 1:2658491/1‑70 (MQ=17) cgGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTAc < 1:5262566/71‑1 (MQ=255) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa > 1:2468543/3‑71 (MQ=18) aaGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt < 1:3885686/71‑1 (MQ=255) aaGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgt > 1:606767/1‑71 (MQ=21) gCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGc < 1:3671484/71‑1 (MQ=21) gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCa < 1:939301/70‑1 (MQ=255) ttgCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa < 1:2801232/59‑1 (MQ=255) gCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAtt < 1:4596660/70‑2 (MQ=21) gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATcc > 1:2122357/1‑71 (MQ=255) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:2698853/71‑1 (MQ=255) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:4818242/1‑70 (MQ=18) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:1423884/1‑70 (MQ=18) tCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:762628/1‑48 (MQ=255) tCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGt < 1:1751831/71‑1 (MQ=21) cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTa < 1:2153166/70‑1 (MQ=18) cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1247214/70‑1 (MQ=255) cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1830344/70‑1 (MQ=255) cGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAg < 1:3673076/71‑1 (MQ=18) gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc > 1:3780197/1‑71 (MQ=255) cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt > 1:4676485/1‑71 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3435211/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3225964/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGt > 1:5027997/1‑62 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:2094292/1‑70 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:4208425/1‑70 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaa < 1:38485/71‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATa > 1:4353166/1‑70 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGAt < 1:2887924/69‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:134099/71‑1 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATACCGTAGGCTGGATaa < 1:3377068/71‑1 (MQ=255) aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaac > 1:2702758/1‑60 (MQ=17) aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa < 1:2166123/71‑2 (MQ=17) gcgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg < 1:2684412/71‑1 (MQ=255) ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcgcga > 1:2501852/1‑70 (MQ=11) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:5423361/1‑64 (MQ=39) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:4402637/1‑64 (MQ=39) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg > 1:694400/1‑65 (MQ=40) tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt < 1:3422101/58‑1 (MQ=35) ggCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt > 1:4950249/1‑57 (MQ=35) gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg < 1:3243313/71‑1 (MQ=39) gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:1443038/55‑1 (MQ=35) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1135395/1‑71 (MQ=39) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1219639/1‑71 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATcct < 1:2760224/71‑2 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1312082/71‑1 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1188101/71‑1 (MQ=39) cgTGACCGCCTTATCCGCCCTACATGTGCAATCCCg < 1:5157192/36‑1 (MQ=255) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:3818596/36‑1 (MQ=40) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:3404298/36‑1 (MQ=40) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg > 1:3390797/1‑71 (MQ=39) gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt > 1:5103668/1‑71 (MQ=38) | TGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAT > Exported/1614459‑1614591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |