Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 309,859 G→A 26.7% T1005I (ACC→ATC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported309,8590GA26.7% 112.4 / 42.9 71T1005I (ACC→ATC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (29/23);  new base A (8/11);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA  >  Exported/309792‑309929
                                                                   |                                                                      
cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                                                     >  1:4126667/1‑71 (MQ=255)
 acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc                                                                    >  1:5554540/1‑71 (MQ=255)
    aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                                  >  1:5162098/1‑70 (MQ=255)
    aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                                  >  1:849491/1‑68 (MQ=12)
     aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                                    <  1:847248/67‑1 (MQ=255)
     aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                                    <  1:625993/67‑1 (MQ=255)
     aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                                    <  1:182003/67‑1 (MQ=255)
      gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag                                                               >  1:2923753/1‑69 (MQ=255)
        acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                                             >  1:3298615/3‑71 (MQ=255)
        acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                                             >  1:4712527/3‑71 (MQ=255)
         tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                                  >  1:5670355/1‑65 (MQ=255)
         tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                                  >  1:2475731/1‑65 (MQ=255)
          aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           <  1:5275557/71‑1 (MQ=255)
            cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                                  >  1:5372972/1‑60 (MQ=255)
             ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                                        >  1:309971/1‑71 (MQ=255)
             ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                                        <  1:2365160/71‑1 (MQ=255)
              gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                                                       <  1:2910880/69‑1 (MQ=255)
              gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                                                       <  1:2736173/69‑1 (MQ=255)
              gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                                                       <  1:2729606/71‑1 (MQ=255)
                aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                                     >  1:2623255/1‑71 (MQ=255)
                 cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                                                    >  1:2352911/1‑71 (MQ=255)
                 cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           >  1:487488/1‑64 (MQ=255)
                 cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           >  1:1336105/1‑64 (MQ=255)
                  gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                                   <  1:4858816/71‑1 (MQ=255)
                  gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                                                   <  1:1760126/71‑1 (MQ=12)
                   aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           <  1:4603109/62‑1 (MQ=255)
                   aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           <  1:1588986/62‑1 (MQ=255)
                   aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                           <  1:2606199/62‑1 (MQ=255)
                     gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                                <  1:1517037/71‑1 (MQ=255)
                     gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                                <  1:1306413/71‑1 (MQ=255)
                      aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa                                               <  1:448472/71‑1 (MQ=255)
                        gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                                             >  1:3420077/1‑71 (MQ=255)
                        gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                                             >  1:3216696/1‑71 (MQ=255)
                        gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                                             <  1:91297/71‑1 (MQ=255)
                         aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                                             <  1:2801117/70‑1 (MQ=255)
                         aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                                                    <  1:4018313/63‑1 (MQ=255)
                         aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                                                    <  1:4069408/63‑1 (MQ=255)
                            gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                                          >  1:4999943/1‑70 (MQ=255)
                                 tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                                    >  1:1582566/1‑71 (MQ=255)
                                  aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                                    >  1:5516986/1‑70 (MQ=255)
                                   ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa                                  >  1:2665121/1‑71 (MQ=255)
                                     gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt                                >  1:1012766/1‑71 (MQ=255)
                                      ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt                                >  1:4175240/1‑70 (MQ=255)
                                         cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt                             <  1:468344/70‑1 (MQ=255)
                                           gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                                            <  1:4097617/37‑1 (MQ=255)
                                           gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                                            <  1:5169313/37‑1 (MQ=255)
                                           gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                                            <  1:4352798/37‑1 (MQ=255)
                                           gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa                           <  1:2850347/70‑1 (MQ=255)
                                            tCACCATCCCGCCCATTACACCGGTACCTACTGCg                                                             <  1:719907/35‑1 (MQ=255)
                                              aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg                       <  1:2622107/71‑1 (MQ=255)
                                                cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc                          >  1:1679130/1‑66 (MQ=255)
                                                  tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGa                                               <  1:1986799/43‑1 (MQ=255)
                                                  tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTg                               >  1:2983748/1‑59 (MQ=255)
                                                  tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg                    >  1:2489622/1‑70 (MQ=255)
                                                    ccGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                                     >  1:4285740/1‑35 (MQ=255)
                                                    ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa                  >  1:2221300/1‑70 (MQ=255)
                                                     cGCCCATTACACCGGTACCTACTGCGTTCTGCGGGCCGGAACCAGCAcc                                      <  1:1454465/49‑1 (MQ=255)
                                                      gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                                          >  1:4182180/1‑44 (MQ=255)
                                                      gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                                          >  1:1062951/1‑44 (MQ=255)
                                                        ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg             >  1:3367986/1‑71 (MQ=255)
                                                          aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           <  1:5062096/71‑1 (MQ=255)
                                                          aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           <  1:4369787/71‑1 (MQ=255)
                                                           ttACCCCGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGcc           >  1:2923481/1‑70 (MQ=255)
                                                            tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           >  1:2110669/1‑69 (MQ=255)
                                                            tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           >  1:2464934/1‑69 (MQ=255)
                                                             acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg        >  1:2989727/1‑71 (MQ=255)
                                                              ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg        <  1:4620256/68‑1 (MQ=255)
                                                              ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg        <  1:3655006/68‑1 (MQ=255)
                                                              cacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa               >  1:3283800/1‑63 (MQ=255)
                                                                 cGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa    <  1:5235571/71‑1 (MQ=255)
                                                                 cGGTACCTACTGCGTTCTCCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt     >  1:1016418/1‑70 (MQ=255)
                                                                  ggTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa   <  1:4032596/71‑1 (MQ=255)
                                                                   gTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa  <  1:5169148/71‑1 (MQ=255)
                                                                   |                                                                      
CACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA  >  Exported/309792‑309929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: