Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,792,350 C→T 14.6% A245V (GCG→GTG) ‡ gabT → 4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,792,3500CT14.6% 113.4 / 10.2 48A245V (GCG→GTG) ‡gabT4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (23/18);  new base T (4/3);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

AGCGTTTACGCGCTCTGTGTGACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAA  >  Exported/1792284‑1792420
                                                                  |                                                                      
aGCGTTTACGCGCTCTGTGTGACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCgggg                                                                    >  1:3037624/1‑71 (MQ=255)
             tctGTGTGACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGc                                                       >  1:1664302/1‑71 (MQ=255)
              ctGTGTGACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTAc                                                               >  1:4993916/1‑62 (MQ=255)
              ctGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCt                                                      <  1:4652432/71‑1 (MQ=255)
               tgtgtgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTg                                                     >  1:4684396/1‑71 (MQ=255)
                 tgtgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTg                                                     <  1:1308500/69‑1 (MQ=255)
                 tgtgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTg                                                     <  1:849431/69‑1 (MQ=255)
                 tgtgACGAGCACGGGATCATGCTGAATGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCt                                                      <  1:4720948/68‑1 (MQ=255)
                  gcgACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGttt                                                  >  1:4831320/3‑71 (MQ=255)
                   tgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCggg                                                                     >  1:1961799/1‑51 (MQ=255)
                   tgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCggg                                                                     >  1:532730/1‑51 (MQ=255)
                   tgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCggg                                                                     >  1:2040951/1‑51 (MQ=255)
                   tgACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCggg                                                                     >  1:4733063/1‑51 (MQ=255)
                    gatgAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGc                                                <  1:893522/68‑1 (MQ=255)
                     aCGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGc                                                <  1:782166/70‑1 (MQ=255)
                     aCGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGc                                                <  1:2704639/70‑1 (MQ=255)
                       gAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGtt                                                   >  1:5575742/1‑65 (MQ=255)
                         gCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATgg                                           <  1:5162724/71‑1 (MQ=255)
                          cACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCgg                                                            >  1:2652280/1‑53 (MQ=255)
                          cACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATg                                            >  1:2924971/1‑69 (MQ=255)
                             gggATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCa                                       <  1:2439660/71‑1 (MQ=255)
                                 tCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTg                                                     <  1:3937224/53‑1 (MQ=255)
                                 tCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATg                                   >  1:606700/1‑71 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGa                                     >  1:757240/1‑65 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGa                                     >  1:1979243/1‑65 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGa                                     >  1:243532/1‑65 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCg                               >  1:23092/1‑71 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCg                               >  1:1003948/1‑71 (MQ=255)
                                     gCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCg                               >  1:3120713/1‑71 (MQ=255)
                                      cTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCg                               <  1:4467188/70‑1 (MQ=255)
                                      cTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGt                              <  1:1239879/71‑1 (MQ=255)
                                       tGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGa                                              >  1:5492579/1‑54 (MQ=255)
                                       tGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGtt                             >  1:62374/1‑71 (MQ=255)
                                         aTTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGtt                             <  1:4786949/69‑1 (MQ=255)
                                          ttGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGa                                              >  1:5322686/1‑51 (MQ=255)
                                              cGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATgg                                           <  1:3804862/50‑1 (MQ=255)
                                              cGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATgg                                           <  1:4517687/50‑1 (MQ=255)
                                              cGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCgg                      <  1:2118988/71‑1 (MQ=255)
                                              cGATGAAGTGCAGAGCGGCGCGGGGAGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCgg                      <  1:4446443/71‑1 (MQ=255)
                                               gATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGa                     >  1:1191120/1‑71 (MQ=255)
                                                aTGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGa                                          >  1:68233/1‑49 (MQ=12)
                                                  gAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCt                  >  1:4795562/1‑71 (MQ=255)
                                                  gAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCt                  >  1:380934/1‑71 (MQ=255)
                                                   atgTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATc                   <  1:4160121/67‑1 (MQ=255)
                                                   aaGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCt                  >  1:3824077/1‑70 (MQ=255)
                                                    aGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGa                     <  1:1930109/66‑1 (MQ=255)
                                                         agagCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTaccacc           <  1:2033011/71‑1 (MQ=255)
                                                         agagCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTaccacc           <  1:3020909/71‑1 (MQ=255)
                                                                cgcgGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGa    <  1:3208125/71‑1 (MQ=255)
                                                                cgcgGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGa    <  1:3796617/71‑1 (MQ=255)
                                                                 gcgGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGa    >  1:3979176/1‑70 (MQ=255)
                                                                  cgGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGaaa  <  1:1877644/71‑1 (MQ=255)
                                                                  |                                                                      
AGCGTTTACGCGCTCTGTGTGACGAGCACGGGATCATGCTGATTGCCGATGAAGTGCAGAGCGGCGCGGGGCGTACCGGCACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAA  >  Exported/1792284‑1792420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: