Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,823,331 G→T 19.0% intergenic (+68/+60) norW → / ← hypF NADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,823,3310GT19.0% 140.9 / 14.0 52intergenic (+68/+60)norW/hypFNADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base T (4/6);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCGTT  >  Exported/1823261‑1823399
                                                                       |                                                                    
tAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtg                                                                      >  1:5614669/1‑71 (MQ=255)
  ggTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtgtg                                                                    <  1:2626366/71‑1 (MQ=255)
   gTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt                                                                   <  1:1008766/71‑1 (MQ=255)
   gTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt                                                                   <  1:5467348/71‑1 (MQ=255)
      ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg                                                                <  1:2687919/71‑1 (MQ=255)
      ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg                                                                >  1:3997628/1‑71 (MQ=255)
           cTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGc                                                               >  1:3974633/1‑67 (MQ=255)
           cTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGa                                                           <  1:2086222/71‑1 (MQ=255)
              tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGAt                                                          <  1:60408/69‑1 (MQ=255)
              tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATaa                                                        <  1:2136262/71‑1 (MQ=255)
               gCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAg                                                       >  1:5442851/1‑71 (MQ=255)
                 cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtgt                                                                     <  1:4765372/55‑1 (MQ=255)
                 cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGc                                                     <  1:839346/71‑1 (MQ=255)
                 cTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGc                                                     <  1:61392/71‑1 (MQ=255)
                   aaaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATgtgt                                                                   <  1:4665323/55‑1 (MQ=255)
                    gggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGc                                                     <  1:3300383/66‑1 (MQ=18)
                    gggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCg                                                    <  1:5224143/67‑1 (MQ=18)
                    aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGtt                                                  <  1:2659720/71‑1 (MQ=255)
                    aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGtt                                                  >  1:3545806/1‑71 (MQ=255)
                    aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGt                                                   <  1:5583964/70‑1 (MQ=255)
                     ggtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt                                                  <  1:2093581/69‑1 (MQ=21)
                      aTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTa                                                >  1:3678413/1‑71 (MQ=255)
                       tGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAc                                               >  1:4195416/1‑71 (MQ=255)
                         tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcg                                              <  1:226097/70‑1 (MQ=255)
                         tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcg                                              <  1:1206975/70‑1 (MQ=255)
                          cGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcgcc                                            >  1:1009605/1‑71 (MQ=255)
                           ggATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcgcc                                            <  1:1886594/70‑1 (MQ=255)
                            gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg                                           <  1:3502571/71‑1 (MQ=18)
                            gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg                                           <  1:2220199/71‑1 (MQ=18)
                             aTGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCg                                                             >  1:106473/1‑51 (MQ=255)
                               gCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt                                        <  1:342817/70‑1 (MQ=255)
                                cGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                       <  1:5260542/70‑1 (MQ=255)
                                cGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                       <  1:2446669/70‑1 (MQ=255)
                                 gACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                      >  1:4586736/1‑71 (MQ=18)
                                            gTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTc                          >  1:3734995/1‑71 (MQ=255)
                                            gTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTc                          >  1:4468053/1‑71 (MQ=255)
                                               ttATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAt                                >  1:2486958/1‑62 (MQ=255)
                                               ttATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAt                                >  1:3773046/1‑62 (MQ=255)
                                                  tCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATg                    >  1:1056081/1‑71 (MQ=255)
                                                   ccGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGgtgt                           >  1:381741/1‑63 (MQ=255)
                                                    cGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt                                                  <  1:521248/40‑1 (MQ=17)
                                                    cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc                   <  1:2103605/70‑1 (MQ=255)
                                                    cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc                   <  1:1676696/70‑1 (MQ=255)
                                                    cGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGCGTCCAAATGc                   <  1:150420/70‑1 (MQ=255)
                                                       ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTa                                                >  1:170489/1‑39 (MQ=14)
                                                        cTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAacac              >  1:3485102/1‑71 (MQ=255)
                                                          aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                            >  1:617926/1‑40 (MQ=11)
                                                          aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                            >  1:2492745/1‑40 (MQ=11)
                                                          aCGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGt            >  1:1425518/1‑71 (MQ=255)
                                                           cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                   >  1:1035101/1‑47 (MQ=255)
                                                           cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                   >  1:4126872/1‑47 (MQ=255)
                                                           cGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACg             <  1:4216816/69‑1 (MQ=255)
                                                                   gCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc                   <  1:2547528/56‑1 (MQ=255)
                                                                   gCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGc                   <  1:5193773/56‑1 (MQ=255)
                                                                   gCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAACCGGCAATGGTGTCCAAATGCAACACGTTTTATCCg    >  1:1937331/1‑71 (MQ=255)
                                                                     aTGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATc      >  1:364538/1‑67 (MQ=255)
                                                                     aTGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCGtt  >  1:2022711/1‑71 (MQ=255)
                                                                     aTGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCGt   >  1:5312209/1‑70 (MQ=255)
                                                                       |                                                                    
TAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCGTT  >  Exported/1823261‑1823399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑