Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,826,243 C→A 29.3% Q27H (CAG→CAT hydN ← formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,826,2430CA29.3% 214.7 / 68.1 109Q27H (CAG→CAThydNformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base C (45/32);  new base A (17/15);  total (62/47)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

Only 100 of 109 total aligned reads displayed.

ACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGAT  >  Exported/1826175‑1826311
                                                                    |                                                                    
acacCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt                                                                     >  1:3577443/1‑70 (MQ=255)
acacCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt                                                                     >  1:4775503/1‑70 (MQ=255)
     tttAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat                                                                  >  1:5689004/1‑68 (MQ=255)
         aTGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGaaa                                                            <  1:4873535/70‑1 (MQ=255)
              aTGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt                                                                     >  1:5190936/1‑56 (MQ=255)
                 gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg                                                   >  1:3602715/1‑71 (MQ=255)
                 gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg                                                   >  1:4261569/1‑71 (MQ=255)
                 gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg                                                   >  1:1488008/1‑71 (MQ=255)
                 gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACg                                                   >  1:3988531/1‑71 (MQ=255)
                     cgcgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGaaa                                                            >  1:4173205/1‑58 (MQ=255)
                         gTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga                                                              <  1:5510429/52‑1 (MQ=255)
                          tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac                                          <  1:612773/71‑1 (MQ=255)
                            aaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat                                                                  <  1:5298269/45‑1 (MQ=255)
                             aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:4945897/71‑1 (MQ=255)
                              aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGt                                      <  1:3883524/71‑1 (MQ=255)
                               aaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTa                                     >  1:2947631/1‑71 (MQ=255)
                                  tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt                                                                     <  1:5361557/36‑1 (MQ=255)
                                  tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt                                                                     <  1:4469653/36‑1 (MQ=255)
                                  tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGt                                      <  1:1239893/67‑1 (MQ=255)
                                   ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg                                  >  1:737801/1‑70 (MQ=255)
                                    tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAAcca                                                         >  1:96347/1‑45 (MQ=255)
                                    tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac                                          >  1:1112419/1‑61 (MQ=255)
                                       ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAAc                              >  1:5412158/1‑70 (MQ=255)
                                       ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAAcc                             <  1:3590309/71‑1 (MQ=255)
                                         ggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa                           <  1:2852499/71‑1 (MQ=255)
                                            cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCa                                                     >  1:2704404/1‑42 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACTCACGCTACTACACAGGTACGGCAACCAATACa                       >  1:508002/1‑71 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTa                                     >  1:3008819/1‑57 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTa                                     >  1:3961321/1‑57 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCaa                           >  1:4462017/1‑67 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCaa                           >  1:4262611/1‑67 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATAc                        <  1:3326430/70‑1 (MQ=255)
                                             aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa                       >  1:1007127/1‑71 (MQ=255)
                                              gCGATGCACAGTCCTGATTTTACTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa                       <  1:765359/70‑1 (MQ=255)
                                               cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt                     >  1:3295559/1‑71 (MQ=255)
                                               cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt                     <  1:1007139/71‑1 (MQ=255)
                                               cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt                     <  1:4151053/71‑1 (MQ=255)
                                                 aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTa                   >  1:1894282/1‑71 (MQ=255)
                                                 aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:4132488/51‑1 (MQ=255)
                                                 aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:966424/51‑1 (MQ=255)
                                                 aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTa                   <  1:1973093/71‑1 (MQ=255)
                                                   gCACAGTCCTGATTTTGCTGATGAGAAACCACGCACGCTACTTCACAGGTa                                     >  1:3382647/1‑51 (MQ=38)
                                                   gCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg                                        >  1:1436344/1‑48 (MQ=255)
                                                   gCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg                                        >  1:4692973/1‑48 (MQ=255)
                                                    cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTAc                                    >  1:2238228/1‑51 (MQ=255)
                                                    cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTAc                                    >  1:2244745/1‑51 (MQ=255)
                                                      caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc              <  1:3192130/68‑1 (MQ=255)
                                                      caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc              <  1:1102946/68‑1 (MQ=255)
                                                      caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc              <  1:4541114/68‑1 (MQ=255)
                                                       aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAgg                                       <  1:1078367/43‑1 (MQ=12)
                                                       aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAgg                                       <  1:2021130/43‑1 (MQ=12)
                                                       aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca             >  1:649410/3‑70 (MQ=255)
                                                       aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc              <  1:2550428/68‑1 (MQ=255)
                                                       aGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:181518/45‑1 (MQ=255)
                                                       aGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:1912060/45‑1 (MQ=255)
                                                        gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg                                       <  1:1449875/44‑1 (MQ=255)
                                                        gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt                 >  1:133643/1‑66 (MQ=255)
                                                        gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt                 >  1:4059290/1‑66 (MQ=255)
                                                        atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat            >  1:1623890/2‑69 (MQ=255)
                                                        atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat            >  1:4393609/2‑69 (MQ=255)
                                                        atCCTGAATCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat            <  1:1165970/70‑3 (MQ=255)
                                                         tCCTGATTTTCCTTATGAGAAACCACGCACGCTACTTCACAGGTACGGc                                 <  1:3634267/49‑1 (MQ=255)
                                                         tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc                                 <  1:5149652/49‑1 (MQ=255)
                                                         tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACg                                   >  1:2740211/1‑47 (MQ=16)
                                                           cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg         >  1:4335482/1‑71 (MQ=255)
                                                           cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg         >  1:880577/1‑71 (MQ=255)
                                                           cTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt                      >  1:1394768/1‑58 (MQ=255)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:3370833/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:5233976/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:168476/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:5239275/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:5273999/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:4594554/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:4574773/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa                                     >  1:386318/1‑43 (MQ=12)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga                           <  1:2051226/53‑3 (MQ=255)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga                           <  1:1103046/53‑3 (MQ=255)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt                      >  1:1124460/1‑58 (MQ=255)
                                                           cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg         >  1:1523518/1‑69 (MQ=255)
                                                            tGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt                 <  1:3074446/62‑1 (MQ=255)
                                                             gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt                 >  1:3799546/1‑61 (MQ=255)
                                                             gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt                 >  1:3681554/1‑61 (MQ=255)
                                                             gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc        >  1:5298515/1‑70 (MQ=255)
                                                             gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa                                <  1:4620319/46‑1 (MQ=16)
                                                             gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa                                <  1:4354397/46‑1 (MQ=16)
                                                             gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa                                <  1:2131427/46‑1 (MQ=16)
                                                             gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc        >  1:4850430/1‑70 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCa                                >  1:1634268/1‑45 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa      >  1:5036326/1‑71 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa       >  1:3737640/1‑70 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa       <  1:2285167/70‑1 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt                      >  1:2524375/1‑55 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg               >  1:515577/1‑62 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc        <  1:1264433/69‑1 (MQ=255)
                                                              aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc        <  1:602642/69‑1 (MQ=255)
                                                              aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc        <  1:4796910/69‑1 (MQ=255)
                                                              aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc        <  1:3267245/69‑1 (MQ=255)
                                                              aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa       <  1:3064599/70‑1 (MQ=255)
                                                                 ttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc                                 <  1:2303942/41‑1 (MQ=255)
                                                                  tCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat  >  1:4631497/1‑71 (MQ=255)
                                                                    |                                                                    
ACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGAT  >  Exported/1826175‑1826311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: