Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,459 A→T 28.7% D320E (GAT→GAA nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4590AT28.7% 156.3 / 79.4 101D320E (GAT→GAAnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base T (17/12);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 117 total aligned reads displayed.

CCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAT  >  Exported/1837392‑1837529
                                                                   |                                                                      
ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                                                       <  1:2586968/69‑1 (MQ=255)
ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                                                       <  1:2746298/69‑1 (MQ=255)
 cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAAtcatca                                                                     >  1:2363276/1‑70 (MQ=255)
 cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                    >  1:3523343/1‑71 (MQ=255)
 cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                    >  1:1830435/1‑71 (MQ=255)
 cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                    >  1:577200/1‑71 (MQ=255)
  gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                    >  1:1943963/1‑70 (MQ=255)
  gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                    >  1:4166284/1‑70 (MQ=255)
   ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                  >  1:1506986/1‑71 (MQ=255)
    cTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                 >  1:3004534/1‑71 (MQ=255)
     ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                                                       <  1:3249820/64‑1 (MQ=255)
       aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                               >  1:3185901/1‑70 (MQ=255)
        aCTTCTTGTTGTTCCCGGACCAGGATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                             <  1:2500831/71‑1 (MQ=255)
        aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                             <  1:5127570/71‑1 (MQ=255)
        aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                             <  1:3627885/71‑1 (MQ=255)
        aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                             <  1:2222326/71‑1 (MQ=255)
        aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                              <  1:877407/70‑1 (MQ=255)
         cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                              <  1:3885381/69‑1 (MQ=255)
         cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                              <  1:2011674/69‑1 (MQ=255)
         cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                              <  1:254132/69‑1 (MQ=255)
               gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                        <  1:5424680/69‑1 (MQ=255)
               gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                       <  1:4205122/70‑1 (MQ=255)
                  gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                          >  1:3393886/1‑64 (MQ=255)
                   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                   <  1:470958/70‑1 (MQ=255)
                   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                   <  1:1129091/70‑1 (MQ=255)
                   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                  <  1:4905618/71‑1 (MQ=255)
                     cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                              >  1:2303730/1‑57 (MQ=255)
                       tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                               <  1:940574/67‑1 (MQ=255)
                       tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                               <  1:216349/67‑1 (MQ=255)
                       cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                                              <  1:2104168/71‑1 (MQ=255)
                            cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                 <  1:918072/47‑1 (MQ=255)
                            cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt                                         <  1:210467/71‑1 (MQ=255)
                             aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt                                         <  1:2273518/70‑1 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                               >  1:4194240/1‑47 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                               >  1:4418962/1‑47 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                               >  1:1609507/1‑47 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                               >  1:3298963/1‑47 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                       <  1:3767932/55‑1 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                       <  1:5178212/55‑1 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                       <  1:760127/55‑1 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                       <  1:4288213/71‑1 (MQ=255)
                              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                       <  1:969777/71‑1 (MQ=255)
                               cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                      >  1:5110646/1‑71 (MQ=255)
                                atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                      >  1:2584178/4‑70 (MQ=255)
                                 ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                  <  1:398584/41‑1 (MQ=255)
                                 ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                          >  1:1587023/1‑49 (MQ=255)
                                 ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                       >  1:1663468/1‑68 (MQ=255)
                                 ttGTGTAGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                  <  1:503388/41‑1 (MQ=255)
                                  tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc                                   >  1:4724543/1‑71 (MQ=255)
                                  tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg                                    >  1:4044360/1‑70 (MQ=255)
                                  tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg                                          >  1:5221032/1‑64 (MQ=255)
                                   gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                  >  1:3937281/1‑71 (MQ=255)
                                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                  >  1:3550347/1‑71 (MQ=255)
                                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                  >  1:2447017/1‑71 (MQ=255)
                                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg                                  >  1:5366931/1‑71 (MQ=255)
                                    tgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                             <  1:4988662/43‑1 (MQ=255)
                                    tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                >  1:3683978/1‑56 (MQ=255)
                                    tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                >  1:744114/1‑56 (MQ=255)
                                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                       >  1:832223/4‑63 (MQ=255)
                                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                <  1:4151842/67‑1 (MQ=255)
                                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                <  1:147037/67‑1 (MQ=255)
                                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                               >  1:4608661/4‑71 (MQ=255)
                                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                               >  1:435145/4‑71 (MQ=255)
                                      tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                                                 <  1:5070809/53‑1 (MQ=255)
                                       cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                           >  1:1162839/3‑58 (MQ=255)
                                       cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                               >  1:5488917/3‑70 (MQ=255)
                                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                 <  1:2391443/35‑1 (MQ=255)
                                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                             >  1:171463/1‑71 (MQ=255)
                                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                             >  1:279509/1‑71 (MQ=255)
                                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga                              >  1:2485721/1‑69 (MQ=255)
                                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                           >  1:3314409/2‑57 (MQ=255)
                                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                   <  1:2958374/64‑3 (MQ=255)
                                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct                             <  1:4579251/70‑2 (MQ=255)
                                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                            <  1:5610259/71‑3 (MQ=255)
                                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                            <  1:2302439/71‑3 (MQ=255)
                                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                            <  1:4749363/71‑3 (MQ=255)
                                         taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                <  1:1184967/65‑1 (MQ=255)
                                          tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc                            >  1:4100491/1‑70 (MQ=255)
                                            tGGGCGTAGGCACTCAGGTAATCATCATTTTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt                          >  1:1414191/1‑70 (MQ=255)
                                            tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt                         <  1:2870711/71‑1 (MQ=255)
                                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa                                                     >  1:3229625/1‑42 (MQ=255)
                                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                    >  1:4626672/1‑43 (MQ=255)
                                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                    >  1:4294273/1‑43 (MQ=255)
                                               gCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                       <  1:1852372/38‑1 (MQ=255)
                                                  tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                  <  1:1068833/40‑1 (MQ=255)
                                                    ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa                  >  1:2287529/1‑70 (MQ=255)
                                                     gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                <  1:1831448/71‑1 (MQ=255)
                                                        cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                      <  1:4423282/62‑1 (MQ=255)
                                                        cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac             <  1:2100593/71‑1 (MQ=255)
                                                          cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa           >  1:4076349/1‑71 (MQ=255)
                                                            ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                       <  1:1838597/41‑1 (MQ=255)
                                                            ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg         >  1:1033989/1‑71 (MQ=255)
                                                             gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                <  1:893411/63‑1 (MQ=255)
                                                               aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac             >  1:5385861/1‑64 (MQ=255)
                                                               aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac             >  1:775615/1‑64 (MQ=255)
                                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                               >  1:3969487/1‑43 (MQ=255)
                                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                               >  1:3919408/1‑43 (MQ=255)
                                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac             <  1:1029486/61‑1 (MQ=255)
                                                                   atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa           >  1:2835029/1‑62 (MQ=255)
                                                                   atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt  <  1:43273/71‑1 (MQ=255)
                                                                   |                                                                      
CCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAT  >  Exported/1837392‑1837529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: