| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,932,982 | G→A | 25.7% | T487T (ACC→ACT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,932,982 | 0 | G | A | 25.7% | 211.5 / 76.9 | 129 | T487T (ACC→ACT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (55/39); new base A (20/13); total (77/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 137 total aligned reads displayed.
CATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTG > Exported/1932912‑1933052 | tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt > 1:2800879/1‑71 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg > 1:1143899/1‑70 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:2485013/70‑1 (MQ=255) gCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg > 1:4501457/1‑61 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGctt < 1:905567/70‑3 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:3173011/70‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:287165/70‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:1281469/70‑1 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3424891/1‑65 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:2810753/1‑71 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:80001/1‑71 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:1140601/70‑1 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4162378/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGTATGGTATGGCTGTTGGTGa > 1:34734/1‑59 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:152248/1‑71 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:2969026/1‑71 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGGTg > 1:723710/1‑55 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:4696342/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5317859/70‑1 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5661682/70‑1 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:469845/70‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa > 1:1195276/1‑52 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:4596429/1‑70 (MQ=255) cGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa > 1:1911385/1‑70 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4976200/55‑1 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:214042/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4480796/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTTTGTAAACAGCATAATCATTCGGt > 1:4371568/1‑62 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4613870/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1222664/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1549438/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1121669/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4542885/1‑70 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3372839/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:1871851/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:195424/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:2515203/1‑71 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:596568/1‑71 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:5675664/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa > 1:2797841/1‑48 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:615725/1‑56 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:1964495/1‑56 (MQ=255) cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa > 1:2007391/1‑62 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:538936/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:166024/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc < 1:1912456/71‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1215495/64‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAccc < 1:2474814/71‑1 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc > 1:4509101/4‑68 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa < 1:4105807/67‑1 (MQ=255) aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:605765/1‑68 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:2554270/1‑71 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:3687344/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:3349004/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:4210291/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:2029828/1‑53 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc < 1:4848811/66‑1 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:539040/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2071754/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:4361469/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1768795/1‑71 (MQ=255) gAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:591399/1‑69 (MQ=255) aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAccag > 1:3842704/1‑69 (MQ=255) aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4995649/1‑66 (MQ=255) aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:5310255/57‑1 (MQ=255) cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:3724019/56‑1 (MQ=255) cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:5224403/56‑1 (MQ=255) gTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa > 1:2743370/1‑52 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg < 1:5073843/71‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1028680/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:4781095/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1262694/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:405258/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg < 1:2874951/66‑2 (MQ=255) gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc < 1:1094365/55‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg < 1:2438293/60‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt < 1:834487/71‑1 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:1655341/1‑70 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:561937/1‑70 (MQ=255) gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:1587734/1‑70 (MQ=255) gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:3022853/1‑70 (MQ=255) tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg < 1:3806969/57‑3 (MQ=255) tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg < 1:2880802/57‑3 (MQ=255) gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt > 1:4266918/1‑71 (MQ=255) gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt > 1:259517/1‑71 (MQ=255) gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt > 1:5237861/1‑70 (MQ=255) cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:2287609/71‑1 (MQ=255) cTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg > 1:3006644/1‑50 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTCTCGGCGGTAAGCCATGTTCCa < 1:227321/70‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:5648073/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:2511018/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:498485/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:692037/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa < 1:2188498/70‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg > 1:106959/1‑71 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGAGGTa > 1:163794/1‑58 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:639598/1‑41 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:3662225/1‑71 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:4782943/1‑71 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:409699/1‑71 (MQ=255) | CATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTG > Exported/1932912‑1933052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |