Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 310,793 T→C 8.5% K694E (AAA→GAA) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported310,7930TC 8.5% 162.0 / 5.2 59K694E (AAA→GAA) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (23/31);  new base C (3/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGA  >  Exported/310723‑310861
                                                                      |                                                                    
cGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                      <  1:4757332/71‑1 (MQ=255)
cGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                      <  1:3711164/71‑1 (MQ=255)
 gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                                     <  1:5607459/71‑1 (MQ=255)
 gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                                     <  1:2909431/71‑1 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                                     <  1:4161674/68‑1 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                  >  1:3586773/1‑71 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                  >  1:3162654/1‑71 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                                 <  1:1106219/71‑1 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                                 <  1:5023481/71‑1 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                                 <  1:4482940/71‑1 (MQ=255)
         gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:295943/71‑1 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCGTGAGTCAGTTTTTCGTGAcc                                                             >  1:280878/1‑69 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:3969444/69‑1 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             >  1:4977237/1‑69 (MQ=255)
              cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                                        <  1:83119/71‑1 (MQ=255)
              cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                                        <  1:5482814/71‑1 (MQ=255)
                tatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                                      <  1:194227/71‑1 (MQ=255)
                       gTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:5345261/57‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa                                            <  1:4248127/71‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa                                            >  1:5678351/1‑71 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                             <  1:4569373/70‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                             <  1:2166150/70‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                      <  1:3740370/43‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                      <  1:1571864/43‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                      <  1:3167609/43‑1 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                           >  1:1054075/1‑69 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                           >  1:1785531/1‑69 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                           >  1:2705732/1‑69 (MQ=255)
                               ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                                   <  1:4555066/43‑1 (MQ=255)
                                tgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                      >  1:3442614/1‑71 (MQ=255)
                                 gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                                 >  1:1755266/1‑43 (MQ=255)
                                 gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                      >  1:1410835/1‑70 (MQ=255)
                                   ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                    >  1:2339927/1‑70 (MQ=255)
                                   ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                    >  1:4169904/1‑70 (MQ=255)
                                    tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcc                                                   >  1:1967700/1‑54 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                                              <  1:1587239/42‑1 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                                  <  1:1220595/70‑1 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                   <  1:817166/69‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                  >  1:3649713/1‑37 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                  >  1:1733112/1‑37 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:5243633/42‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:70925/42‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                             <  1:1351816/42‑1 (MQ=255)
                                       gcaagcaaCTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                                            >  1:1807289/1‑42 (MQ=255)
                                       gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                                            >  1:2489477/1‑42 (MQ=255)
                                       ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc                               <  1:1626494/70‑1 (MQ=255)
                                         aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga                             <  1:999838/71‑3 (MQ=255)
                                         aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa                             <  1:2247475/71‑1 (MQ=255)
                                         aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa                             <  1:1283897/71‑1 (MQ=255)
                                          agcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga                             >  1:1523076/1‑68 (MQ=255)
                                           gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa                           >  1:4088067/1‑70 (MQ=255)
                                           gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa                           >  1:1711755/1‑70 (MQ=255)
                                           gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag                           >  1:425769/1‑71 (MQ=255)
                                            caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc                          >  1:4575506/1‑71 (MQ=255)
                                            caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc                          >  1:2708401/1‑71 (MQ=255)
                                             aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                         <  1:5341281/71‑1 (MQ=255)
                                               cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                                                 <  1:5583754/45‑1 (MQ=255)
                                               cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                           >  1:436058/1‑51 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                   <  1:2018286/56‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                   <  1:835662/56‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                    <  1:4700272/71‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                    <  1:4797019/71‑1 (MQ=255)
                                                    tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                    >  1:4778008/1‑69 (MQ=255)
                                                          ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc            <  1:577185/71‑1 (MQ=255)
                                                          ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc            <  1:3974914/71‑1 (MQ=255)
                                                              aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt        <  1:2198278/71‑1 (MQ=255)
                                                                   gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc                                     <  1:3457405/37‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                         <  1:2744478/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                         <  1:4875957/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                         <  1:3020273/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                         <  1:383746/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                    <  1:1198118/53‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc                   <  1:1731220/54‑1 (MQ=255)
                                                                     ttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGa  >  1:2639938/1‑70 (MQ=255)
                                                                      tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                              >  1:2049266/1‑41 (MQ=255)
                                                                      tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                              >  1:1446223/1‑41 (MQ=255)
                                                                      tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa                  >  1:4645013/1‑53 (MQ=255)
                                                                      tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGt         >  1:4281216/1‑62 (MQ=255)
                                                                      |                                                                    
CGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGA  >  Exported/310723‑310861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: