| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,054 | G→A | 19.7% | T463T (ACC→ACT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,054 | 0 | G | A | 19.7% | 457.1 / 94.5 | 213 | T463T (ACC→ACT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (96/75); new base A (28/14); total (124/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
Only 100 of 220 total aligned reads displayed.
TGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATC > Exported/1932984‑1933119 | tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg < 1:594551/71‑1 (MQ=255)tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:837025/1‑71 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:244594/71‑1 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:4710148/71‑1 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGt > 1:1117808/1‑71 (MQ=255) gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:3103808/1‑65 (MQ=255) cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg > 1:379263/1‑70 (MQ=255) aTTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:222314/71‑1 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:1038074/1‑70 (MQ=255) atcATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATc > 1:1327360/3‑71 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:4487654/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:4741292/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2562783/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCt < 1:3005456/71‑1 (MQ=255) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTaa > 1:489146/4‑66 (MQ=12) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTc < 1:4864590/68‑1 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:4060993/1‑70 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:3637554/71‑1 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:2721361/71‑1 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTGAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4605625/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4403023/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa < 1:4850848/71‑1 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:2249762/1‑71 (MQ=255) ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:5649709/1‑71 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:3678290/1‑69 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:3344681/1‑69 (MQ=255) cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:4360687/1‑68 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTa > 1:177889/1‑69 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:3623786/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:2414069/70‑1 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:715726/1‑70 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:2935772/71‑1 (MQ=255) tACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa > 1:3374853/1‑70 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:276887/1‑61 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:3031612/1‑71 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa < 1:5325187/70‑1 (MQ=255) atacccAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGc > 1:5622597/4‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2187071/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2034544/1‑71 (MQ=255) tcacAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:227026/4‑71 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:2434104/1‑71 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:2157477/1‑71 (MQ=255) cccAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc < 1:5442157/71‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2074851/48‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:3304704/51‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:679348/1‑53 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:4010927/1‑53 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:2018189/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:3930457/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:1584344/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgt > 1:4269045/1‑70 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:1659818/42‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:413791/42‑1 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:3667897/1‑71 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:623682/1‑62 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt > 1:1489893/1‑71 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:4159028/1‑62 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:4344229/1‑62 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5508692/1‑61 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg < 1:282302/69‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg < 1:374490/69‑1 (MQ=255) ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:5089658/1‑71 (MQ=255) gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:132381/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc < 1:1524349/44‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:1561213/52‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:3262295/71‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2332597/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:863496/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:141426/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:5427428/71‑1 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:5192428/1‑71 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1314586/1‑38 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3367552/1‑51 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5667727/1‑51 (MQ=255) ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc < 1:1729618/47‑1 (MQ=255) ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagcatt < 1:2202938/71‑4 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:2885321/1‑49 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc > 1:2967475/1‑70 (MQ=255) cATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:1107454/1‑39 (MQ=12) aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa > 1:1084495/1‑71 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:3339026/60‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg < 1:3178810/66‑1 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt > 1:3531122/1‑71 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:3405306/1‑35 (MQ=12) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:3308526/1‑60 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:1858649/1‑60 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:2725835/1‑60 (MQ=255) tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:3768674/1‑36 (MQ=255) tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg > 1:2664724/1‑71 (MQ=255) ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa > 1:369527/1‑71 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCttta > 1:693925/1‑58 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc > 1:2511602/1‑61 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt > 1:3222538/1‑70 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCtt > 1:546473/1‑38 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt < 1:2131891/71‑3 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:5014813/44‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:476922/1‑70 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt < 1:1154704/54‑1 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt < 1:1386820/54‑1 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:4422532/52‑1 (MQ=255) | TGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATC > Exported/1932984‑1933119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |