| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,121 | A→T | 40.1% | L441Q (CTG→CAG) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,121 | 0 | A | T | 40.1% | 120.0 / 198.4 | 167 | L441Q (CTG→CAG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (44/56); new base T (27/40); total (71/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 169 total aligned reads displayed.
GTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAT > Exported/1933055‑1933191 | caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac > 1:3999744/2‑69 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg < 1:2627342/71‑1 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg > 1:5455186/1‑71 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:4591973/70‑1 (MQ=255) cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt < 1:344419/71‑1 (MQ=255) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg < 1:251110/69‑1 (MQ=255) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:1957255/71‑1 (MQ=255) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt > 1:2153822/1‑71 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:2108966/68‑1 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:5420870/68‑1 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt < 1:2769100/68‑1 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:5333516/1‑71 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc < 1:1217528/70‑1 (MQ=255) tCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg < 1:3560192/65‑1 (MQ=255) cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc > 1:5031790/1‑71 (MQ=255) ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaa > 1:3260059/1‑60 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc > 1:1685781/1‑70 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt > 1:2063233/1‑71 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGaa < 1:3334116/58‑1 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt < 1:5634821/69‑1 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:3157432/70‑1 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa > 1:728693/1‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt < 1:557120/57‑1 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt > 1:4084607/2‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt > 1:504192/2‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt > 1:3321430/2‑70 (MQ=255) tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:3810730/58‑1 (MQ=255) tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:1038443/58‑1 (MQ=255) tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc < 1:5379538/71‑1 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg > 1:5693059/1‑69 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg > 1:157900/1‑69 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct < 1:4171349/70‑2 (MQ=255) gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg > 1:4037584/1‑71 (MQ=255) aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgc < 1:2846754/71‑1 (MQ=255) cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt > 1:2141943/1‑71 (MQ=255) cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt < 1:4430440/71‑1 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:3133129/1‑52 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:3622461/58‑1 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:4142501/58‑1 (MQ=255) tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:4602582/69‑2 (MQ=255) tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:304919/69‑2 (MQ=255) tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa < 1:3810493/71‑1 (MQ=255) ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcgt > 1:3469952/2‑69 (MQ=255) ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:567082/69‑2 (MQ=255) gCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt > 1:2250125/1‑61 (MQ=255) cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGtt < 1:51440/48‑1 (MQ=255) cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt > 1:2837605/1‑57 (MQ=255) cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc < 1:2975721/71‑1 (MQ=255) tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt < 1:1668496/69‑2 (MQ=255) tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt < 1:102543/69‑2 (MQ=255) cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa < 1:185405/70‑4 (MQ=255) cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa < 1:5573518/70‑4 (MQ=255) cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttta < 1:1583054/69‑3 (MQ=255) gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa > 1:2656606/1‑66 (MQ=255) gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa > 1:3920543/1‑66 (MQ=255) tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc > 1:2887221/1‑71 (MQ=255) tcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTc < 1:4628986/62‑1 (MQ=255) cgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc < 1:2014241/55‑1 (MQ=255) gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAg < 1:4729331/37‑1 (MQ=255) tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt < 1:1432638/71‑1 (MQ=255) tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt < 1:5443102/71‑1 (MQ=255) cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc < 1:3043267/70‑1 (MQ=255) tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:5461608/1‑70 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:2278565/1‑71 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:3019898/1‑71 (MQ=255) tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc > 1:1110760/1‑71 (MQ=255) cgcgGCAACCTGATCCAGGACGCGTTGCGCCTGATc > 1:5638580/1‑36 (MQ=255) gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg > 1:4927850/1‑54 (MQ=255) gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg > 1:3878992/1‑54 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:234870/71‑1 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:4699661/71‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt < 1:5163388/60‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc < 1:1364007/69‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:5256005/70‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:1425217/54‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:2442884/54‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:2479176/54‑1 (MQ=255) cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa < 1:440975/41‑1 (MQ=255) cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa < 1:3350585/41‑1 (MQ=255) cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:5270196/1‑71 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa < 1:677582/40‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:1558429/62‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:3734483/71‑1 (MQ=255) aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc < 1:1230944/38‑1 (MQ=255) aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc < 1:3042499/38‑1 (MQ=255) aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt > 1:2066139/1‑41 (MQ=255) cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc > 1:2684931/1‑40 (MQ=255) tGATCCAGGAAGCGTTGCGCTTGATCTTCCGCGTCATTc < 1:1818849/39‑1 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:571443/3‑71 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:3179684/3‑71 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:5614661/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:2515325/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg < 1:545302/50‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:394704/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc < 1:3514483/68‑1 (MQ=255) aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:178907/57‑1 (MQ=255) aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac < 1:1624623/71‑1 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg > 1:3529392/1‑51 (MQ=255) ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt > 1:2960401/1‑58 (MQ=255) aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:43334/71‑1 (MQ=255) | GTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAT > Exported/1933055‑1933191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |