Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,198 C→T 48.6% E415E (GAG→GAA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,1980CT48.6% 13.1 / 140.4 107E415E (GAG→GAAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (31/24);  new base T (20/32);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 8.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGA  >  Exported/1933129‑1933264
                                                                     |                                                                  
ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACt                                                                   <  1:5328050/71‑1 (MQ=255)
  gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc                                                                  >  1:5641659/1‑70 (MQ=255)
  cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc                                                                  >  1:1032166/3‑70 (MQ=255)
  cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc                                                                  >  1:4696600/3‑70 (MQ=255)
   agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                                                                <  1:4686757/70‑1 (MQ=255)
   agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                                                                <  1:4362054/70‑1 (MQ=255)
   agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCa                                                                 <  1:3045636/69‑1 (MQ=255)
    gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa                                                                 >  1:1285278/1‑69 (MQ=255)
    gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa                                                                 >  1:300300/1‑69 (MQ=255)
      cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc                                                             >  1:1817262/1‑71 (MQ=255)
       tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg                                                            >  1:3995602/1‑71 (MQ=255)
        gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg                                                           >  1:579612/1‑71 (MQ=255)
         aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt                                                          >  1:5260041/1‑71 (MQ=255)
           cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc                                                         >  1:162298/1‑70 (MQ=255)
           cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                        >  1:83250/1‑71 (MQ=255)
            ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg                                                       >  1:5093520/1‑69 (MQ=255)
              ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt                                                          <  1:3795329/66‑1 (MQ=255)
              ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc                                                         <  1:4732972/67‑1 (MQ=255)
              ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt                                                      <  1:148358/70‑1 (MQ=255)
              ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                        <  1:181014/68‑1 (MQ=255)
              ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                        <  1:3784642/68‑1 (MQ=255)
               cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg                                                     <  1:5053157/70‑1 (MQ=255)
                 cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAtt                                                  <  1:1958619/71‑1 (MQ=255)
                   tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt                                                 <  1:2735161/70‑1 (MQ=255)
                    cATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc                                                         <  1:1971917/61‑1 (MQ=255)
                     aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc                                               <  1:1360530/70‑1 (MQ=255)
                       tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg                                            >  1:2018706/1‑71 (MQ=255)
                       tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg                                            >  1:4314297/1‑71 (MQ=255)
                         aGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt                                           >  1:3826387/1‑70 (MQ=255)
                         aGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt                                           >  1:5368628/1‑70 (MQ=255)
                          attCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt                                          <  1:4205209/68‑1 (MQ=255)
                           ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTc                                                <  1:3011458/62‑1 (MQ=255)
                           ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa                                        >  1:1234982/2‑71 (MQ=255)
                            tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc                                       >  1:3613736/1‑71 (MQ=255)
                            tCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                                                                <  1:3771975/46‑1 (MQ=255)
                             cGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaaaa                                                              >  1:4452849/1‑46 (MQ=255)
                              gCTAAAACCATTACCGATTTTACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat                                     <  1:4925232/71‑3 (MQ=255)
                              gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat                                     <  1:321381/71‑3 (MQ=255)
                              gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAca                                      <  1:5627787/70‑2 (MQ=255)
                                 aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                  >  1:2585388/1‑69 (MQ=255)
                                 aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                  >  1:2156035/1‑69 (MQ=255)
                                 aaaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt                                  >  1:5640703/1‑71 (MQ=255)
                                   aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc                                                             <  1:5349877/42‑1 (MQ=255)
                                    gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                               >  1:2357625/1‑71 (MQ=255)
                                    gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                               >  1:3459425/1‑71 (MQ=255)
                                    gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                               >  1:3650974/1‑71 (MQ=255)
                                    gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                               >  1:1998655/1‑71 (MQ=255)
                                      cattACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg                             <  1:217585/69‑2 (MQ=255)
                                        ttAGCGATTTCACGACCACCGATGAAGAATTCAAAACg                                                            <  1:5633858/38‑1 (MQ=25)
                                        ttACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                  <  1:4769723/64‑3 (MQ=255)
                                        ttACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt                                                 >  1:1390382/1‑49 (MQ=255)
                                         tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                    >  1:4416406/1‑45 (MQ=255)
                                         tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                    >  1:5626423/1‑45 (MQ=255)
                                         tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                    >  1:3099723/1‑45 (MQ=255)
                                         gaCCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                    >  1:5177432/2‑45 (MQ=255)
                                          aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAt                                                   >  1:3840602/1‑45 (MQ=255)
                                          aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg                         >  1:1279968/1‑71 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                  >  1:1087368/1‑56 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                     <  1:5182046/71‑1 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                     <  1:5034452/71‑1 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                     <  1:784231/71‑1 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                     <  1:4415099/71‑1 (MQ=255)
                                              aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                     >  1:610317/1‑71 (MQ=255)
                                              aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg                                                     <  1:5181708/39‑1 (MQ=255)
                                               tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg                         <  1:4283486/66‑3 (MQ=255)
                                               tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg                         <  1:1903804/66‑3 (MQ=255)
                                               tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc                    <  1:4345069/71‑1 (MQ=255)
                                               tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc                    <  1:114508/71‑1 (MQ=255)
                                               tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                    <  1:1902831/71‑1 (MQ=255)
                                               tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                    <  1:2494489/71‑1 (MQ=255)
                                                ttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAc                                       >  1:1764189/1‑51 (MQ=255)
                                                ttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAc                                       >  1:170271/1‑51 (MQ=255)
                                                 tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGcc                       >  1:3604451/1‑66 (MQ=255)
                                                 tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg                   <  1:2327593/70‑1 (MQ=255)
                                                 tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg                   <  1:5217669/70‑1 (MQ=255)
                                                  cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg                   <  1:1954216/69‑1 (MQ=255)
                                                   aCGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt                                  >  1:4191302/1‑53 (MQ=255)
                                                    cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGGCAGCGgag                >  1:4861846/1‑70 (MQ=255)
                                                    cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag                >  1:2485440/1‑70 (MQ=255)
                                                     gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCTTGCCAGCGgggac              <  1:4978718/71‑2 (MQ=255)
                                                      accaccAATGAAGAACTCTAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                               <  1:5526757/53‑1 (MQ=255)
                                                       ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc            >  1:1928819/1‑71 (MQ=255)
                                                       ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc            >  1:1245625/1‑71 (MQ=255)
                                                            gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa                                        >  1:168162/2‑38 (MQ=25)
                                                            gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg       <  1:5022301/70‑1 (MQ=255)
                                                            actGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt        <  1:5183383/68‑1 (MQ=255)
                                                            aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                       >  1:3819135/1‑55 (MQ=255)
                                                            aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                       >  1:5497285/1‑55 (MQ=255)
                                                             aTGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt                                          <  1:1059066/35‑1 (MQ=25)
                                                             aTGAAGAATTCAAAACGGGCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGc      <  1:162148/71‑1 (MQ=255)
                                                              tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt        <  1:3702050/68‑1 (MQ=255)
                                                               gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc            <  1:1030113/63‑1 (MQ=255)
                                                               gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc            <  1:3077125/63‑1 (MQ=255)
                                                                aagaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg    <  1:4965131/70‑1 (MQ=255)
                                                                aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAAc                                       >  1:3454373/1‑35 (MQ=255)
                                                                aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg   <  1:3482038/71‑1 (MQ=255)
                                                                aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg    >  1:2914009/1‑70 (MQ=255)
                                                                 agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc            <  1:4604216/61‑1 (MQ=255)
                                                                  gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTac                               <  1:3750045/41‑1 (MQ=19)
                                                                  gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa  >  1:2674907/1‑70 (MQ=255)
                                                                     |                                                                  
TTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGA  >  Exported/1933129‑1933264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: