Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,282 C→T 36.1% P387P (CCG→CCA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,2820CT36.1% 125.9 / 119.2 130P387P (CCG→CCAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (38/45);  new base T (26/21);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 131 total aligned reads displayed.

TGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAG  >  Exported/1933212‑1933348
                                                                      |                                                                  
tgATTTCCGGGTTAACGTCGTTACGACGCGCAAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc                                                                    >  1:4597413/1‑71 (MQ=255)
       cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa                                                             >  1:239370/1‑71 (MQ=255)
          gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                                                            >  1:5534655/1‑69 (MQ=255)
          gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa                                                          <  1:4157015/71‑1 (MQ=255)
          gTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa                                                          <  1:5486266/71‑1 (MQ=255)
           ttAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAg                                                         <  1:2243293/71‑1 (MQ=255)
             aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa                                                             >  1:5617751/1‑65 (MQ=255)
             aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa                                                             >  1:1530850/1‑65 (MQ=255)
             aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt                                                       >  1:4055516/1‑71 (MQ=255)
                   gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt                                                 >  1:2449454/1‑71 (MQ=255)
                   attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt                                                 <  1:187248/70‑1 (MQ=255)
                    ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTc                                                >  1:2088010/1‑71 (MQ=255)
                     tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa                                                          <  1:4351435/60‑1 (MQ=255)
                      acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTg                                              >  1:3141325/1‑71 (MQ=255)
                          cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc                                          <  1:2829183/71‑1 (MQ=255)
                           gcgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                                                            <  1:5121648/52‑1 (MQ=255)
                             gcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtc                                       >  1:4819275/1‑71 (MQ=255)
                              ccAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtct                                      >  1:243279/1‑71 (MQ=255)
                               cAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt                                                 <  1:366448/59‑1 (MQ=255)
                                 gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCActtcata                                    >  1:4625207/1‑67 (MQ=255)
                                 gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc                                    >  1:137987/1‑70 (MQ=255)
                                 gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg                                   <  1:4607680/71‑1 (MQ=255)
                                 gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg                                   <  1:934750/71‑1 (MQ=255)
                                  cGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc                                            <  1:336913/61‑1 (MQ=255)
                                    gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc                                          >  1:2806325/1‑61 (MQ=255)
                                      gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc                                                   <  1:1816081/50‑1 (MQ=255)
                                      gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt                              <  1:1602002/71‑1 (MQ=255)
                                       aaaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc                             <  1:3332629/71‑1 (MQ=255)
                                        caCTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGa                                                        >  1:4206593/2‑43 (MQ=25)
                                        aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct                            >  1:4452494/1‑71 (MQ=255)
                                         aCTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa                                                             >  1:686656/1‑37 (MQ=25)
                                         aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtctc                           >  1:3067115/1‑71 (MQ=255)
                                          cTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGc                                                   <  1:2199425/46‑1 (MQ=37)
                                          cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg                                <  1:157479/65‑1 (MQ=255)
                                          cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg                                <  1:5651827/65‑1 (MQ=255)
                                          cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg                                <  1:125500/65‑1 (MQ=255)
                                           ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGc                                                   <  1:4701199/45‑1 (MQ=38)
                                           ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGc                                                   <  1:4661222/45‑1 (MQ=38)
                                           ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCaaa                                 >  1:4159003/1‑63 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa                                                        >  1:2596696/1‑40 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                                       >  1:3000191/1‑41 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                                       >  1:3007052/1‑41 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                                       >  1:5221359/1‑41 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg                         >  1:659157/1‑71 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg                         >  1:746224/1‑71 (MQ=255)
                                           ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGAGCTTCTGCCACTTCTTCGAAGATCTCgg                         >  1:2016147/1‑71 (MQ=255)
                                            tCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt                                                 >  1:1080521/1‑46 (MQ=255)
                                            tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt                              >  1:3369534/1‑65 (MQ=255)
                                            tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt                              >  1:5461863/1‑65 (MQ=255)
                                            tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt                              >  1:4072003/1‑65 (MQ=255)
                                            tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt                              >  1:2316289/1‑65 (MQ=255)
                                             cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt                                                       >  1:2297552/1‑39 (MQ=37)
                                             cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt                                                       >  1:1191669/1‑39 (MQ=37)
                                             cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt                                                       >  1:1123035/1‑39 (MQ=37)
                                             cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt                        >  1:1264060/1‑70 (MQ=255)
                                             cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt                        <  1:5263079/70‑1 (MQ=255)
                                                ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc                     <  1:3331715/70‑1 (MQ=255)
                                                ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc                     <  1:5251145/70‑1 (MQ=255)
                                                ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc                     <  1:5257695/70‑1 (MQ=255)
                                                ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc                     <  1:2198002/70‑1 (MQ=255)
                                                ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACg                    <  1:2950561/71‑1 (MQ=255)
                                                 cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa                   <  1:3931061/71‑3 (MQ=255)
                                                 cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa                   <  1:3572823/71‑3 (MQ=255)
                                                  ggATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAAt                  <  1:2151697/71‑1 (MQ=255)
                                                  ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt                  <  1:4125749/71‑1 (MQ=255)
                                                    atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc                >  1:2914735/1‑71 (MQ=255)
                                                    atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc                >  1:5397678/1‑71 (MQ=255)
                                                    atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc                <  1:1503976/71‑1 (MQ=255)
                                                      atTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGaa                                 >  1:1627492/1‑52 (MQ=255)
                                                       ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt                  <  1:3361860/66‑1 (MQ=255)
                                                        tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCt                                               >  1:3963131/1‑36 (MQ=255)
                                                        tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa                                  <  1:2718328/49‑1 (MQ=255)
                                                        tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa                                  <  1:5684663/49‑1 (MQ=255)
                                                        tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc            >  1:3587386/1‑71 (MQ=255)
                                                        tCAGTAATGAAGGTCGGATGAATCAGATGTGCTTCTGCCACTTCTTCGa                                  <  1:3886211/49‑1 (MQ=255)
                                                         ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc            >  1:880591/3‑70 (MQ=255)
                                                         ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc            >  1:228746/3‑70 (MQ=255)
                                                         cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg                          >  1:4408036/1‑56 (MQ=255)
                                                         cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc            >  1:1606530/1‑70 (MQ=255)
                                                          cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc           <  1:5349380/69‑1 (MQ=255)
                                                          cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc           <  1:3444668/69‑1 (MQ=255)
                                                          cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc            >  1:4073074/2‑69 (MQ=255)
                                                          aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa          <  1:3773351/71‑1 (MQ=255)
                                                            taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca                                   <  1:2489814/44‑2 (MQ=37)
                                                            taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaac        <  1:2622100/71‑3 (MQ=255)
                                                             aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg                                <  1:3744015/46‑1 (MQ=255)
                                                             aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa        <  1:3029277/70‑1 (MQ=255)
                                                             aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc       >  1:3738040/1‑71 (MQ=255)
                                                              aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc      >  1:2666226/1‑71 (MQ=255)
                                                              aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc      >  1:5092796/1‑71 (MQ=255)
                                                               tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc                             <  1:3058260/47‑1 (MQ=255)
                                                               tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa                   <  1:171847/57‑1 (MQ=255)
                                                               tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa                   <  1:1088435/57‑1 (MQ=255)
                                                               tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc            <  1:3814991/64‑1 (MQ=255)
                                                                 aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctct                          >  1:3028351/1‑47 (MQ=37)
                                                                 aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa                 >  1:2208617/1‑57 (MQ=255)
                                                                 aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa                 >  1:4037722/1‑57 (MQ=255)
                                                                 aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa          >  1:25551/1‑64 (MQ=255)
                                                                 aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc     >  1:1942019/1‑69 (MQ=255)
                                                                     tCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa                   >  1:3669043/1‑51 (MQ=255)
                                                                      |                                                                  
TGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAG  >  Exported/1933212‑1933348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: