| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,363 | G→A | 8.7% | I360I (ATC→ATT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,363 | 0 | G | A | 8.7% | 327.5 / 14.1 | 115 | I360I (ATC→ATT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (62/43); new base A (5/5); total (67/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGG > Exported/1933293‑1933431 | gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg < 1:4687913/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5369291/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5632467/71‑1 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:2393523/1‑71 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:2899991/1‑71 (MQ=255) tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:1089477/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1034932/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:2069040/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1875216/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1536290/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:3481753/1‑70 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa < 1:2313055/69‑1 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:5553026/1‑69 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:548779/1‑69 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:296842/1‑70 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:2793419/1‑70 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3662298/70‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:1923287/54‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:1316710/64‑1 (MQ=255) tcGAATATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:2191541/1‑71 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:2928213/63‑1 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:693485/1‑71 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:1137033/1‑71 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:3270839/1‑66 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:4023790/71‑1 (MQ=255) aTCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCtt > 1:2622580/1‑70 (MQ=255) ctctGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttta > 1:2088103/1‑69 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:476645/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:1343296/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:2012272/1‑68 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg < 1:4826876/68‑1 (MQ=255) gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga < 1:5367815/71‑1 (MQ=255) tAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCagag > 1:3408136/1‑71 (MQ=255) gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:2944620/51‑1 (MQ=255) gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3530729/51‑1 (MQ=255) gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa < 1:5642619/71‑1 (MQ=255) aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCt > 1:2604011/1‑57 (MQ=255) aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa < 1:5498546/70‑1 (MQ=255) tACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGc > 1:702205/1‑61 (MQ=255) tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg < 1:3860992/71‑1 (MQ=255) aCGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCagcat > 1:3577424/1‑62 (MQ=255) aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa > 1:5191741/1‑61 (MQ=255) aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:518802/1‑70 (MQ=255) aCGGCCCAGACCCAAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:4195335/1‑70 (MQ=255) cccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:1401028/1‑54 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg < 1:3319813/48‑1 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:5408165/1‑66 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4405054/1‑66 (MQ=255) cAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa < 1:950847/71‑1 (MQ=255) aGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:4230118/51‑1 (MQ=255) gACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa < 1:4739081/71‑1 (MQ=255) acccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAgg > 1:5018149/3‑70 (MQ=255) aCCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:2405484/39‑1 (MQ=255) ccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCag < 1:4788319/52‑1 (MQ=255) cccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttt < 1:1370876/46‑1 (MQ=255) cccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttt < 1:58127/46‑1 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:2962336/37‑1 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:4195063/1‑47 (MQ=255) ccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTcgg < 1:4201/71‑3 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa < 1:5521836/49‑1 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:3136553/57‑1 (MQ=255) cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCaa > 1:5626434/1‑37 (MQ=255) cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc > 1:1890944/1‑68 (MQ=255) gCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCg < 1:274392/52‑1 (MQ=255) ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:2692205/57‑1 (MQ=255) tcttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa > 1:695690/1‑51 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:36992/1‑68 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:4641696/1‑68 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt > 1:3269418/1‑71 (MQ=255) ttctCAACGTGGATGCCTATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:692277/69‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:2939179/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:1048956/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:647785/69‑1 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:166091/1‑42 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa > 1:2435580/1‑57 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg > 1:5657053/1‑71 (MQ=255) ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt < 1:377594/68‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:3458363/1‑36 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:821347/1‑48 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:1956897/1‑48 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:2974341/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:4149875/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:2378936/50‑1 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCTGtt > 1:4926143/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt > 1:3457524/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCCAAGTTGTCCAGATCCGCCATGTCGGtt > 1:1021309/1‑71 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa > 1:4579636/1‑45 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:3205432/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:741757/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4585788/1‑47 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:213676/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:5230752/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAg > 1:1268317/1‑52 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc > 1:1187861/1‑59 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg > 1:3955385/1‑66 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:3351043/45‑1 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg < 1:3970705/71‑1 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg > 1:518343/1‑71 (MQ=255) ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:3871441/40‑1 (MQ=255) ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:860913/40‑1 (MQ=255) | GATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGG > Exported/1933293‑1933431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |