| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,381 | A→T | 9.5% | I354I (ATT→ATA) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,381 | 0 | A | T | 9.5% | 350.4 / 22.3 | 126 | I354I (ATT→ATA) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (65/49); new base T (7/5); total (72/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
TCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTT > Exported/1933313‑1933451 | tcGAATATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:2191541/1‑71 (MQ=255)tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:1137033/1‑71 (MQ=255) aaGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGc < 1:5280438/71‑1 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:4023790/71‑1 (MQ=255) aTCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCtt > 1:2622580/1‑70 (MQ=255) ctctGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttta > 1:2088103/1‑69 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:476645/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:1343296/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:3711795/1‑68 (MQ=255) gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga < 1:5367815/71‑1 (MQ=255) gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa < 1:5642619/71‑1 (MQ=255) aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa < 1:5498546/70‑1 (MQ=255) tACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGc > 1:702205/1‑61 (MQ=255) tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg < 1:3860992/71‑1 (MQ=255) aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa > 1:5191741/1‑61 (MQ=255) aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:518802/1‑70 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg < 1:1940396/48‑1 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg < 1:3319813/48‑1 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4405054/1‑66 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:5408165/1‑66 (MQ=255) cAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa < 1:950847/71‑1 (MQ=255) aGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:4230118/51‑1 (MQ=255) gACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa < 1:4739081/71‑1 (MQ=255) ccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCag < 1:4788319/52‑1 (MQ=255) cccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttt < 1:1370876/46‑1 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:1268140/45‑1 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:5667421/1‑47 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:4195063/1‑47 (MQ=255) ccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTcgg < 1:4201/71‑3 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa < 1:5521836/49‑1 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:2878740/57‑1 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:3136553/57‑1 (MQ=255) cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc > 1:1890944/1‑68 (MQ=255) aGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGc > 1:147769/1‑70 (MQ=255) ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:2692205/57‑1 (MQ=255) tcttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa > 1:695690/1‑51 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:4641696/1‑68 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:36992/1‑68 (MQ=255) ttctCAACGTGGATGCCTATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:692277/69‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:1048956/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:2939179/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:647785/69‑1 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:166091/1‑42 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa > 1:2435580/1‑57 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg > 1:5657053/1‑71 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTACGCag > 1:5523015/1‑42 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:3458363/1‑36 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:3988614/1‑36 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:1956897/1‑48 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:3285343/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:2974341/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:4149875/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt > 1:4995381/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCTGtt > 1:4926143/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt > 1:95204/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt > 1:3457524/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCCAAGTTGTCCAGATCCGCCATGTCGGtt > 1:1021309/1‑71 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa > 1:4579636/1‑45 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:3205432/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:741757/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4585788/1‑47 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:5230752/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:213676/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:2269137/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAg > 1:1268317/1‑52 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg > 1:3955385/1‑66 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:3351043/45‑1 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg < 1:3970705/71‑1 (MQ=255) ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:3871441/40‑1 (MQ=255) ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:860913/40‑1 (MQ=255) aTGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt < 1:3434576/63‑1 (MQ=255) aTGCCGATAGATTCAGCAATTGCTTTCGAAGAGTCGAAGTTGTCCAGATCCGCCATGT‑GGTTTCCGGGCGa < 1:1434767/71‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt < 1:4489459/57‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt < 1:4301297/57‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt < 1:3428074/62‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa > 1:2398714/1‑71 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa < 1:1231949/71‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa < 1:629326/71‑1 (MQ=255) gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt < 1:1167094/41‑1 (MQ=255) gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt < 1:1653556/41‑1 (MQ=255) ccGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGat > 1:5072366/1‑70 (MQ=255) aTAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATAttt > 1:2745621/1‑71 (MQ=255) aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt > 1:1935151/1‑37 (MQ=255) aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa < 1:4420847/42‑1 (MQ=255) aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATAtt > 1:4161495/1‑70 (MQ=255) aTAGATTCAGAAATTGCTTTCGCAGAGTCGAAGTTGt > 1:5258750/1‑37 (MQ=255) aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa > 1:2706683/1‑40 (MQ=255) aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCt > 1:4346231/1‑71 (MQ=255) gATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt < 1:1784528/71‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTc < 1:3740711/68‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg < 1:2807241/71‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg < 1:623735/71‑1 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:1778605/1‑54 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:1314032/1‑54 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGc > 1:4962893/1‑58 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt > 1:5022324/1‑68 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt > 1:254912/1‑71 (MQ=255) cAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc > 1:4039130/1‑41 (MQ=255) aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCtt > 1:5128272/1‑71 (MQ=255) aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCt > 1:497388/1‑70 (MQ=255) | TCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTT > Exported/1933313‑1933451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |