Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,390 C→A 5.5% A351A (GCG→GCT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,3900CA 5.5% 411.9 / 5.1 125A351A (GCG→GCTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (63/55);  new base A (5/2);  total (68/57)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 136 total aligned reads displayed.

GGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGG  >  Exported/1933324‑1933460
                                                                  |                                                                      
ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        <  1:1343296/67‑1 (MQ=255)
ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg                                                                       >  1:3711795/1‑68 (MQ=255)
ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg                                                                       >  1:2012272/1‑68 (MQ=255)
ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg                                                                       <  1:4826876/68‑1 (MQ=255)
 gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga                                                                   <  1:5367815/71‑1 (MQ=255)
        tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                                                            <  1:3860992/71‑1 (MQ=255)
         aCGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCagcat                                                                 >  1:3577424/1‑62 (MQ=255)
         aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa                                                                     >  1:5191741/1‑61 (MQ=255)
             cccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:1401028/1‑54 (MQ=255)
              ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                                                           >  1:4405054/1‑66 (MQ=255)
              ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                                                           >  1:5408165/1‑66 (MQ=255)
               cAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa                                                     <  1:950847/71‑1 (MQ=255)
                aGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        <  1:4230118/51‑1 (MQ=255)
                 gACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa                                                   <  1:4739081/71‑1 (MQ=255)
                   ccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCag                                                                    <  1:4788319/52‑1 (MQ=255)
                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:4195063/1‑47 (MQ=255)
                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:5667421/1‑47 (MQ=255)
                     ccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTcgg                                               <  1:4201/71‑3 (MQ=255)
                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa                                                                     <  1:5521836/49‑1 (MQ=255)
                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                                             <  1:3136553/57‑1 (MQ=255)
                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                                             <  1:2878740/57‑1 (MQ=255)
                      cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:4329494/1‑45 (MQ=255)
                      cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc                                                 >  1:1890944/1‑68 (MQ=255)
                       aGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGc                                              >  1:147769/1‑70 (MQ=255)
                        gCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCg                                                               <  1:274392/52‑1 (MQ=255)
                         ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg                                                         <  1:2692205/57‑1 (MQ=255)
                          tcttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa                                                              >  1:695690/1‑51 (MQ=255)
                           cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa                                            >  1:4641696/1‑68 (MQ=255)
                           cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa                                            >  1:36992/1‑68 (MQ=255)
                            ttctCAACGTGGATGCCTATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg                                          <  1:692277/69‑1 (MQ=255)
                            ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                                             <  1:2939179/50‑1 (MQ=255)
                            ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                                             <  1:1048956/50‑1 (MQ=255)
                            ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg                                          <  1:647785/69‑1 (MQ=255)
                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg                                       >  1:5657053/1‑71 (MQ=255)
                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTACGCag                                                                    >  1:5523015/1‑42 (MQ=255)
                              ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt                                         <  1:377594/68‑1 (MQ=255)
                              ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg                                      >  1:4724252/1‑71 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:3988614/1‑36 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                                                        >  1:2967431/1‑36 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                                                            >  1:821347/1‑48 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                                                          <  1:2378936/50‑1 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                                                          <  1:3285343/50‑1 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                                                          <  1:4149875/50‑1 (MQ=255)
                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt                                     >  1:4995381/1‑71 (MQ=255)
                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCTGtt                                    >  1:4926143/1‑71 (MQ=255)
                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt                                    >  1:3457524/1‑71 (MQ=255)
                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt                                    >  1:95204/1‑71 (MQ=255)
                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCCAAGTTGTCCAGATCCGCCATGTCGGtt                                    >  1:1021309/1‑71 (MQ=255)
                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                                             >  1:4579636/1‑45 (MQ=255)
                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                                                           >  1:741757/1‑47 (MQ=255)
                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                                                           >  1:3205432/1‑47 (MQ=255)
                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                                                           >  1:4585788/1‑47 (MQ=255)
                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag                                                                    >  1:5230752/1‑36 (MQ=255)
                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag                                                                    >  1:213676/1‑36 (MQ=255)
                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAg                                                    >  1:1268317/1‑52 (MQ=255)
                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg                                      >  1:3955385/1‑66 (MQ=255)
                                     tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg                                                         <  1:3351043/45‑1 (MQ=255)
                                     tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg                               >  1:518343/1‑71 (MQ=255)
                                        aTGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt                                    <  1:3434576/63‑1 (MQ=255)
                                        aTGCCGATAGATTCAGCAATTGCTTTCGAAGAGTCGAAGTTGTCCAGATCCGCCATGTGGTTTCCGGGCGa                           <  1:1434767/71‑1 (MQ=255)
                                         tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt                                         <  1:4489459/57‑1 (MQ=255)
                                         tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt                                    <  1:3428074/62‑1 (MQ=255)
                                         tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa                           >  1:2398714/1‑71 (MQ=255)
                                         tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa                           <  1:1231949/71‑1 (MQ=255)
                                          gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt                                                        <  1:1653556/41‑1 (MQ=255)
                                          gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt                                                        <  1:1167094/41‑1 (MQ=255)
                                           ccGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGat                          >  1:5072366/1‑70 (MQ=255)
                                              aTAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATAttt                      >  1:2745621/1‑71 (MQ=255)
                                              aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt                                                        >  1:1935151/1‑37 (MQ=255)
                                              aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATAtt                       >  1:4161495/1‑70 (MQ=255)
                                                aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc                                                 >  1:1927055/1‑42 (MQ=255)
                                                aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCt                    >  1:4346231/1‑71 (MQ=255)
                                                aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTACAGa                                                   >  1:5257507/1‑40 (MQ=255)
                                                 gATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt                   <  1:1784528/71‑1 (MQ=255)
                                                  aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 <  1:3965987/56‑1 (MQ=255)
                                                  aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg                  <  1:623735/71‑1 (MQ=255)
                                                  aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg                  <  1:2807241/71‑1 (MQ=255)
                                                   ttCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 <  1:5408720/55‑1 (MQ=255)
                                                    tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 >  1:1314032/1‑54 (MQ=255)
                                                    tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 >  1:1778605/1‑54 (MQ=255)
                                                    tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 >  1:1752849/1‑54 (MQ=255)
                                                    tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt                   >  1:5022324/1‑68 (MQ=255)
                                                    tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt                >  1:254912/1‑71 (MQ=255)
                                                     cAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc                                             >  1:4039130/1‑41 (MQ=255)
                                                         aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCtt           >  1:5128272/1‑71 (MQ=255)
                                                         aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCt            >  1:497388/1‑70 (MQ=255)
                                                           ttGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt                                     <  1:4502156/43‑1 (MQ=255)
                                                           ttGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCg                                <  1:1975309/48‑1 (MQ=255)
                                                             gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc                                 >  1:3163867/1‑45 (MQ=255)
                                                             gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt                >  1:725789/1‑62 (MQ=255)
                                                             gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg       >  1:4677404/1‑71 (MQ=255)
                                                              cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg                                       >  1:3785249/1‑38 (MQ=255)
                                                              cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt                   >  1:1587487/1‑58 (MQ=255)
                                                              cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg       <  1:5186628/70‑1 (MQ=255)
                                                              cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg       <  1:1188248/70‑1 (MQ=255)
                                                              cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGc      <  1:4297489/71‑1 (MQ=255)
                                                                ttCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt                >  1:4093508/1‑59 (MQ=255)
                                                                 tCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa                           <  1:1319858/47‑1 (MQ=255)
                                                                 tCGCAGAGTCGAACTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCAt    <  1:2628132/70‑1 (MQ=255)
                                                                  cGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATgg  >  1:5047525/1‑71 (MQ=255)
                                                                  |                                                                      
GGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGG  >  Exported/1933324‑1933460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: