| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,633 | A→G | 30.1% | R270R (CGT→CGC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,633 | 0 | A | G | 30.1% | 239.8 / 134.8 | 177 | R270R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (60/63); new base G (25/28); total (85/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 179 total aligned reads displayed.
AGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCA > Exported/1933565‑1933703 | aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:2144389/1‑71 (MQ=255)aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1631772/1‑71 (MQ=255)aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1337695/1‑71 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:5580556/1‑71 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:2618209/69‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:4813837/69‑1 (MQ=255) taaGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2749264/68‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa > 1:5617105/1‑70 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:5237807/57‑1 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:1190534/57‑1 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:4159908/4‑71 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTa > 1:4402170/1‑57 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:3072758/1‑53 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:3745819/70‑1 (MQ=255) tAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTca < 1:4238562/69‑2 (MQ=255) tAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTca < 1:2503967/69‑2 (MQ=255) aTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa > 1:3950377/1‑59 (MQ=255) aTCCGCATACGCCATGTAGAGTTACATCATTGTGAACTCAGGATTATGGCGAACAGaa > 1:4041847/1‑58 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:46491/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:3010245/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:2944428/69‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg < 1:5230058/71‑1 (MQ=255) tacgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGtt < 1:4557750/68‑1 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3607038/47‑1 (MQ=255) aaGCCATGTAGATTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:3969084/1‑71 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa > 1:1549854/1‑52 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2409599/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2954998/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:166163/71‑1 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg > 1:4784605/1‑71 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1890924/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:4256620/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:3692779/1‑56 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:3725946/1‑56 (MQ=255) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa < 1:4776305/44‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3789488/42‑1 (MQ=16) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:1446979/1‑44 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa > 1:1369690/1‑43 (MQ=16) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:880187/1‑35 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4208479/1‑43 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAcc < 1:650793/47‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAcc < 1:1531179/47‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1783731/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:5302943/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:3599301/1‑71 (MQ=255) agagTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt < 1:1984488/70‑1 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:1417008/1‑47 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:3508873/1‑47 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3840340/71‑1 (MQ=255) tCCATCATGGTGAACTATGGGTTATGACGTACGGAAATACCTTCGtt < 1:725456/47‑1 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa < 1:2076415/70‑1 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa > 1:194962/1‑70 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:4123391/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:1003256/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2682370/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:1314845/1‑66 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:2290000/1‑66 (MQ=255) catcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGtt < 1:4010804/54‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5653391/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:4511490/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5484193/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5546947/62‑1 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATacc > 1:1618597/1‑70 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:1116171/61‑1 (MQ=255) catGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATacac > 1:336618/1‑71 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:5339757/1‑71 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa < 1:2793424/62‑1 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:780594/1‑69 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:675221/70‑1 (MQ=255) gTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgtt > 1:1080564/1‑68 (MQ=255) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACgg < 1:1758720/41‑1 (MQ=16) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:91075/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1251588/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4827354/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:430150/71‑1 (MQ=255) aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTcaa > 1:3317304/1‑68 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:5547161/55‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:2892083/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:5195268/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:4084731/71‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:5368982/42‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4519719/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4923416/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:4228905/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:2777119/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:5437985/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:1602887/68‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAAGGTTGATTTCGAATACACGCTCGAAGccac > 1:2157608/1‑71 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:1013563/1‑38 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2616005/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:4676412/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:4870123/70‑1 (MQ=255) gattATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcc > 1:4934209/3‑70 (MQ=255) attATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:2875523/2‑49 (MQ=37) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACg > 1:5447087/1‑36 (MQ=19) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa > 1:277397/1‑60 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa > 1:748797/1‑69 (MQ=255) tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:4435687/1‑63 (MQ=255) tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:5042931/1‑63 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2818589/71‑1 (MQ=255) | AGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCA > Exported/1933565‑1933703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |