| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,651 | G→A | 29.3% | N264N (AAC→AAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,651 | 0 | G | A | 29.3% | 245.2 / 117.8 | 167 | N264N (AAC→AAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (54/64); new base A (23/26); total (77/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 168 total aligned reads displayed.
TGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAC > Exported/1933581‑1933717 | tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:3745819/70‑1 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:3010245/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:2944428/69‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg < 1:5230058/71‑1 (MQ=255) tacgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGtt < 1:4557750/68‑1 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:1962743/69‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa < 1:125228/70‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2409599/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2954998/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:1531389/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:166163/71‑1 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg > 1:5644200/1‑71 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg < 1:1362355/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:165428/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:3501760/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:4256620/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:5021681/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:3725946/1‑56 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt < 1:4017126/71‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1783731/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:3602202/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:1807225/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:3599301/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:5302943/1‑71 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc > 1:565703/1‑71 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3840340/71‑1 (MQ=255) tCCATCATGGTGAACTATGGGTTATGACGTACGGAAATACCTTCGtt < 1:725456/47‑1 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa > 1:194962/1‑70 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa < 1:2076415/70‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:1003256/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:4123391/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2682370/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:1314845/1‑66 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAACTTCGTTACGGaa > 1:4739015/1‑52 (MQ=255) acatcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2891802/2‑52 (MQ=255) catcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGtt < 1:4010804/54‑1 (MQ=255) catcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGtt < 1:1451763/60‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5546947/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5484193/62‑1 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:2115306/61‑1 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:4177917/1‑71 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:5339757/1‑71 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa < 1:2793424/62‑1 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:780594/1‑69 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:675221/70‑1 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:3970881/70‑1 (MQ=255) gTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgtt > 1:1080564/1‑68 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:1146341/71‑1 (MQ=255) tGAACTCAGGATTATGTCGAACAGAAATACCTTCATTACgg < 1:480592/41‑1 (MQ=255) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACgg < 1:1758720/41‑1 (MQ=16) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTca < 1:554714/71‑2 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:4582207/1‑70 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1251588/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:5358803/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:91075/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4827354/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:430150/71‑1 (MQ=255) aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTcaa > 1:3317304/1‑68 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:5195268/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:4084731/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:4649937/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:5368982/42‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4923416/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4519719/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:5437985/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:1408252/1‑71 (MQ=255) tGGGTTATCACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:1613899/42‑1 (MQ=255) gggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTACGAATACACGCTCGAAGccac > 1:2774212/1‑70 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:4676412/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2616005/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:4870123/70‑1 (MQ=255) gTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaa > 1:2757253/1‑71 (MQ=255) ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcct > 1:2235956/1‑68 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACg > 1:5447087/1‑36 (MQ=19) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa > 1:748797/1‑69 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctaca < 1:1084518/70‑2 (MQ=255) tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:4435687/1‑63 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2818589/71‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:1002749/36‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:1033978/1‑71 (MQ=255) cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt < 1:4399028/69‑2 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4410153/52‑1 (MQ=255) agAAATACCTTCATTACGGAAGTTACGGTTGATTTCg < 1:4227035/36‑1 (MQ=38) gAAATACCTTCATTACGGAAGTTATGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3821128/1‑71 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:3268977/47‑1 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:376949/1‑53 (MQ=255) aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa < 1:2298181/71‑1 (MQ=255) tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga < 1:260610/71‑2 (MQ=255) tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga < 1:5118977/71‑2 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTacaac > 1:1873826/1‑56 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa < 1:4856855/71‑1 (MQ=255) cTTCGTTACGGAAGTTTCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:3647018/71‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacca < 1:3893942/49‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGtg > 1:1564411/1‑68 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTa > 1:604968/1‑69 (MQ=255) ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:2430177/1‑69 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:5012709/1‑37 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:2656446/1‑37 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:1972426/1‑44 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcc > 1:2008424/1‑43 (MQ=255) | TGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAC > Exported/1933581‑1933717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |