| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,684 | C→T | 35.1% | E253E (GAG→GAA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,684 | 0 | C | T | 35.1% | 135.4 / 116.9 | 131 | E253E (GAG→GAA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (43/42); new base T (30/16); total (73/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
GTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCA > Exported/1933616‑1933754 | gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:3970881/70‑1 (MQ=255)gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:675221/70‑1 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:1146341/71‑1 (MQ=255) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTca < 1:554714/71‑2 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:4582207/1‑70 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:430150/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4527674/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4827354/71‑1 (MQ=255) aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTcaa < 1:2987779/70‑3 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:2892083/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:4084731/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:5195268/71‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4519719/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4923416/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:1408252/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:4228905/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:2777119/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:1602887/68‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAAGGTTGATTTCGAATACACGCTCGAAGccac > 1:2157608/1‑71 (MQ=255) gggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTACGAATACACGCTCGAAGccac > 1:2774212/1‑70 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2616005/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:4870123/70‑1 (MQ=255) ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgccta > 1:4819371/1‑68 (MQ=255) ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctac < 1:3838952/71‑1 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa > 1:277397/1‑60 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa > 1:748797/1‑69 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctaca < 1:1084518/70‑2 (MQ=255) tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:5042931/1‑63 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:1326860/1‑69 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2818589/71‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAg < 1:1516433/71‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:1033978/1‑71 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4410153/52‑1 (MQ=255) gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGgcacc > 1:421049/1‑56 (MQ=255) gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt > 1:2675331/1‑71 (MQ=255) gAAATACCTTCATTACGGAAGTTATGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3821128/1‑71 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:3268977/47‑1 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:376949/1‑53 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:1239092/1‑53 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg < 1:4666974/71‑1 (MQ=255) aTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:5186520/1‑68 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa > 1:3994674/1‑53 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTacaac > 1:1873826/1‑56 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa < 1:4856855/71‑1 (MQ=255) cTTCGTTACGGAAGTTTCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:3647018/71‑1 (MQ=255) cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:187276/71‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacca < 1:3893942/49‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacca < 1:5360544/49‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTa > 1:604968/1‑69 (MQ=255) ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAcc < 1:1435071/45‑1 (MQ=25) cattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc > 1:4430100/3‑70 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:5012709/1‑37 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:2656446/1‑37 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcc > 1:2008424/1‑43 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAgg > 1:1963701/1‑65 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc < 1:1080444/69‑2 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc < 1:2252117/69‑2 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTA‑AACCAGACGTTTCAGATACAGCTc > 1:2459265/2‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcc > 1:282983/1‑42 (MQ=19) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTcc > 1:5351536/1‑70 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg > 1:5008196/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc < 1:2260078/70‑1 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:3371058/1‑71 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:5596705/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:5252360/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:4202604/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:917003/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg > 1:1566166/1‑62 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc < 1:2875600/71‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc < 1:288199/71‑1 (MQ=255) gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGa < 1:4795269/71‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:515468/51‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:3097117/51‑1 (MQ=255) tACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa > 1:2469760/1‑71 (MQ=255) aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt > 1:2275108/1‑45 (MQ=12) aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa > 1:4185953/1‑70 (MQ=255) aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:5387034/1‑66 (MQ=255) cGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACg > 1:4656395/1‑71 (MQ=255) ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACG‑TTCAGATACAGCTCCGGCGCGATACGCa < 1:201603/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3248809/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:5289292/71‑1 (MQ=255) ttGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa < 1:665385/70‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3631369/1‑71 (MQ=255) aTTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa < 1:4404712/71‑1 (MQ=255) ttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:2757169/1‑71 (MQ=255) tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:490592/1‑70 (MQ=255) tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:3019741/1‑70 (MQ=255) tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:4662182/1‑37 (MQ=255) tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:2582511/1‑37 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:4741747/1‑69 (MQ=255) acGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt < 1:2723919/71‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:2664894/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:1303601/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:1057842/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga < 1:3263218/69‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc < 1:5599401/71‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc < 1:2306737/71‑1 (MQ=255) gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc < 1:3558803/71‑1 (MQ=255) cTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa < 1:2373747/71‑1 (MQ=255) cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc > 1:1373156/1‑52 (MQ=255) | GTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCA > Exported/1933616‑1933754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |