| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,699 | C→A | 33.2% | V248V (GTG→GTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,699 | 0 | C | A | 33.2% | 147.4 / 64.9 | 93 | V248V (GTG→GTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (31/31); new base A (20/11); total (51/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTG > Exported/1933631‑1933767 | tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:3773230/71‑1 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:1326860/1‑69 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2818589/71‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAg < 1:1516433/71‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:1033978/1‑71 (MQ=255) cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt < 1:4399028/69‑2 (MQ=255) gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt > 1:2675331/1‑71 (MQ=255) gAAATACCTTCATTACGGAAGTTATGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3821128/1‑71 (MQ=255) aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa < 1:2298181/71‑1 (MQ=255) aTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:5186520/1‑68 (MQ=255) tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga < 1:260610/71‑2 (MQ=255) ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa < 1:4856855/71‑1 (MQ=255) cTTCGTTACGGAAGTTTCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:3647018/71‑1 (MQ=255) cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:187276/71‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGtg > 1:1564411/1‑68 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTa > 1:604968/1‑69 (MQ=255) ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:2430177/1‑69 (MQ=255) cattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc > 1:4430100/3‑70 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAgg > 1:1963701/1‑65 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgg < 1:5207271/69‑2 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc < 1:1080444/69‑2 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc < 1:2252117/69‑2 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTA‑AACCAGACGTTTCAGATACAGCTc > 1:2459265/2‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTcc > 1:5351536/1‑70 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg > 1:5008196/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAg > 1:421668/1‑60 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc < 1:2260078/70‑1 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:3371058/1‑71 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:4202604/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:917003/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:5252360/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:5596705/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:2971219/1‑59 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg > 1:1566166/1‑62 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc < 1:2875600/71‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc < 1:288199/71‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCA‑CAACCACCAGACGCTTGAGGTACAg > 1:1682360/1‑61 (MQ=255) gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGa < 1:4795269/71‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:515468/51‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:3189958/51‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:4129044/51‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt < 1:3097117/51‑1 (MQ=255) tACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa > 1:2469760/1‑71 (MQ=255) aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa > 1:4185953/1‑70 (MQ=255) aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATAc > 1:4862806/1‑71 (MQ=255) cGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACg > 1:4656395/1‑71 (MQ=255) ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACG‑TTCAGATACAGCTCCGGCGCGATACGCa < 1:201603/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:1084355/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3931902/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:5289292/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3248809/71‑1 (MQ=255) ttGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa < 1:665385/70‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3631369/1‑71 (MQ=255) gATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg > 1:481697/1‑51 (MQ=255) aTTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa < 1:4404712/71‑1 (MQ=255) ttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:2757169/1‑71 (MQ=255) tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:490592/1‑70 (MQ=255) tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa > 1:3019741/1‑70 (MQ=255) tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:4662182/1‑37 (MQ=255) tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:2582511/1‑37 (MQ=255) cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg > 1:2840604/1‑71 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:4741747/1‑69 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:5660354/1‑67 (MQ=255) acGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt < 1:2723919/71‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:1057842/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:1303601/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc > 1:2671457/1‑60 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga < 1:3263218/69‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc < 1:2306737/71‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc < 1:5599401/71‑1 (MQ=255) gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc < 1:3558803/71‑1 (MQ=255) gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc < 1:925502/71‑1 (MQ=255) cTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa < 1:2373747/71‑1 (MQ=255) cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc > 1:2788999/1‑52 (MQ=255) cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc > 1:1373156/1‑52 (MQ=255) cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc > 1:1380682/1‑52 (MQ=255) tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc < 1:1940562/38‑1 (MQ=255) cGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:935990/1‑57 (MQ=255) gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg < 1:446745/61‑1 (MQ=255) gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg < 1:1307289/69‑1 (MQ=255) aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg > 1:3754388/1‑71 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt < 1:5160679/71‑1 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt > 1:1466483/1‑71 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt < 1:530615/71‑1 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:307660/58‑1 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:2140913/58‑1 (MQ=255) caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:252778/1‑49 (MQ=255) accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt < 1:1891689/71‑1 (MQ=255) accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt > 1:2121995/1‑71 (MQ=255) caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg < 1:5127902/71‑1 (MQ=255) caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATACAGCGCGTTAtgg < 1:2083800/71‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGa > 1:5087035/1‑35 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:149809/39‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:1845592/39‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:4778200/39‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc > 1:1710353/1‑61 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc > 1:3788737/1‑61 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt > 1:768292/1‑71 (MQ=255) accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg < 1:4447946/71‑1 (MQ=255) caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga > 1:1355163/1‑50 (MQ=255) | TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTG > Exported/1933631‑1933767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |