Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,756 C→A 23.8% A229A (GCG→GCT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,7560CA23.8% 245.6 / 51.9 109A229A (GCG→GCTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (34/49);  new base A (17/9);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 4.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

AGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCA  >  Exported/1933689‑1933825
                                                                   |                                                                     
aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                                    >  1:97304/1‑71 (MQ=255)
aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                                    >  1:3754388/1‑71 (MQ=255)
 gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                                   >  1:1466483/1‑71 (MQ=255)
 gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                                   <  1:530615/71‑1 (MQ=255)
    accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                                <  1:1891689/71‑1 (MQ=255)
    accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                                >  1:2121995/1‑71 (MQ=255)
      caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                              <  1:5127902/71‑1 (MQ=255)
      caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATACAGCGCGTTAtgg                                                              <  1:2083800/71‑1 (MQ=255)
       aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc                                                                       >  1:1710353/1‑61 (MQ=255)
       aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                             >  1:768292/1‑71 (MQ=255)
        accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                            <  1:4447946/71‑1 (MQ=255)
        acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                            >  1:4548195/4‑71 (MQ=255)
        acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                            >  1:4321830/4‑71 (MQ=255)
         caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                           >  1:1068133/3‑71 (MQ=255)
           accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACGTATCTAAATCCAGAGCATTATgatggg                                                          >  1:3721280/1‑70 (MQ=255)
           accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                         <  1:2784532/71‑1 (MQ=255)
           accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                          >  1:829866/1‑70 (MQ=255)
           accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                         >  1:5677978/1‑71 (MQ=255)
           accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                         <  1:3568469/71‑1 (MQ=255)
            ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                         >  1:1133008/1‑70 (MQ=255)
            ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                         >  1:739177/1‑70 (MQ=255)
            ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                          >  1:4618509/1‑69 (MQ=255)
             caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                                    <  1:1430205/58‑1 (MQ=255)
              aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                       >  1:1790977/1‑70 (MQ=255)
               gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                                      >  1:3131719/1‑70 (MQ=255)
               gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                     <  1:3421319/71‑1 (MQ=255)
               gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                     <  1:585195/71‑1 (MQ=255)
                aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                                    <  1:1714656/71‑1 (MQ=255)
                  gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                  <  1:4374755/71‑1 (MQ=255)
                  gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                  >  1:5113055/1‑71 (MQ=255)
                      gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                <  1:743625/69‑1 (MQ=255)
                      gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                <  1:4481914/69‑1 (MQ=255)
                       agatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcg                                             <  1:4996120/68‑1 (MQ=255)
                         atACAGCTCCGGCGCGATACGCATATACATATCTAAATCCAGAGCATTAt                                                                <  1:280149/49‑1 (MQ=255)
                         atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAt                                                                <  1:4654202/49‑1 (MQ=255)
                         atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                           >  1:5531915/2‑69 (MQ=255)
                          tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                           >  1:5297841/1‑70 (MQ=255)
                          tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                           >  1:1524639/1‑70 (MQ=255)
                          tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                          <  1:3213693/71‑1 (MQ=255)
                           aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                              >  1:1514538/1‑50 (MQ=255)
                           aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                           >  1:4957127/1‑69 (MQ=255)
                            cAGTTCCGGTGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                         <  1:2054571/70‑1 (MQ=255)
                            cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                                   >  1:5344057/1‑60 (MQ=255)
                            cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                        <  1:2744063/71‑1 (MQ=255)
                            cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                        <  1:4187216/71‑1 (MQ=255)
                            cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                        >  1:730371/1‑71 (MQ=255)
                            cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                         <  1:1520136/70‑1 (MQ=255)
                            cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                         <  1:3098146/70‑1 (MQ=255)
                              gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                      >  1:2763614/1‑71 (MQ=255)
                              gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                      <  1:1366105/71‑1 (MQ=255)
                               ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                     <  1:3277419/55‑1 (MQ=255)
                                tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                                     >  1:3631966/1‑70 (MQ=255)
                                tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                                     >  1:1682187/1‑70 (MQ=255)
                                 ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                                    <  1:3232740/70‑1 (MQ=255)
                                 ccGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAga                                       >  1:2668014/1‑66 (MQ=255)
                                  cGGCGCGATTCGCAGGTACATGTCGAGATCCAGCGCGt                                                                   <  1:1514816/38‑1 (MQ=255)
                                  cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                                   <  1:4096482/38‑1 (MQ=255)
                                  cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                                  >  1:37593/1‑71 (MQ=255)
                                  cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgg                                                              >  1:5159091/1‑43 (MQ=16)
                                  cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtga                                                              >  1:4880795/1‑42 (MQ=16)
                                  cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcga                                            <  1:4585500/61‑2 (MQ=255)
                                    gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc                                              <  1:2283399/57‑1 (MQ=255)
                                     cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGCTAAACGGACGCGCAGCGGCACCGc                               <  1:5365137/71‑1 (MQ=255)
                                        gATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                                <  1:2186273/35‑1 (MQ=255)
                                        gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                            <  1:1766010/71‑1 (MQ=255)
                                        gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                            <  1:1173550/71‑1 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                     <  1:1623944/45‑1 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                 <  1:2549212/49‑1 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                 <  1:886589/49‑1 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                           >  1:3536593/1‑71 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                           >  1:4463565/1‑71 (MQ=255)
                                         aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                           >  1:2634568/1‑71 (MQ=255)
                                          tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                       >  1:4060995/1‑42 (MQ=255)
                                          tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                       >  1:5393793/1‑42 (MQ=255)
                                          tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                              <  1:3841223/67‑1 (MQ=255)
                                            cGCAGGTACATGTCGAGATCCAGCGCGTTATTGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                         <  1:827530/70‑1 (MQ=255)
                                            cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                             >  1:2929089/1‑66 (MQ=255)
                                            cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                         <  1:3943651/70‑1 (MQ=255)
                                              cAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCa                      <  1:2229378/71‑1 (MQ=255)
                                               aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc                     <  1:1902217/71‑1 (MQ=255)
                                                ggTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                 <  1:167878/42‑1 (MQ=255)
                                                 gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                   <  1:2411358/71‑1 (MQ=255)
                                                  tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                          <  1:4856365/47‑1 (MQ=255)
                                                  tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTg                  >  1:3909127/1‑71 (MQ=255)
                                                    cATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                  <  1:196368/37‑1 (MQ=255)
                                                     aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat               >  1:3289528/1‑71 (MQ=255)
                                                     aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGca                >  1:5083439/1‑70 (MQ=255)
                                                      tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                           <  1:1338097/42‑1 (MQ=255)
                                                      tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatc              <  1:2953586/71‑1 (MQ=255)
                                                        tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                              <  1:2705047/53‑1 (MQ=255)
                                                        tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatca             <  1:3846328/70‑1 (MQ=255)
                                                          gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                   >  1:2118789/1‑62 (MQ=255)
                                                          gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg          >  1:1910079/1‑71 (MQ=255)
                                                           agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                                    >  1:5679920/1‑44 (MQ=255)
                                                           agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                                    >  1:755035/1‑44 (MQ=255)
                                                             aTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc        >  1:218407/1‑70 (MQ=255)
                                                               ccAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAcc                    >  1:4806698/1‑56 (MQ=255)
                                                                 aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc   <  1:299525/71‑1 (MQ=255)
                                                                  gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc   >  1:1235064/1‑70 (MQ=255)
                                                                  gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCa  <  1:2873253/71‑1 (MQ=255)
                                                                   |                                                                     
AGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCA  >  Exported/1933689‑1933825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: