Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,765 G→A 14.7% H226H (CAC→CAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,7650GA14.7% 213.3 / 23.3 88H226H (CAC→CATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (34/41);  new base A (7/6);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAA  >  Exported/1933695‑1933835
                                                                      |                                                                      
caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                                        <  1:5127902/71‑1 (MQ=255)
caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATACAGCGCGTTAtgg                                                                        <  1:2083800/71‑1 (MQ=255)
 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                                       >  1:768292/1‑71 (MQ=255)
  accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                                      <  1:4447946/71‑1 (MQ=255)
  accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                                        >  1:1444606/1‑69 (MQ=255)
  acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                                      >  1:4548195/4‑71 (MQ=255)
  acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                                      >  1:4321830/4‑71 (MQ=255)
   caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                                     >  1:1068133/3‑71 (MQ=255)
     accaGACGTTTCAGATACAGCTCCGGTGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                                    >  1:415251/1‑70 (MQ=255)
     accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                                   <  1:2784532/71‑1 (MQ=255)
     accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                                    >  1:829866/1‑70 (MQ=255)
     accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                                    >  1:3398683/1‑70 (MQ=255)
     accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                                   >  1:5677978/1‑71 (MQ=255)
     accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                                   <  1:3568469/71‑1 (MQ=255)
      ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                                   >  1:1133008/1‑70 (MQ=255)
      ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                                    >  1:4618509/1‑69 (MQ=255)
        aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                                 >  1:1790977/1‑70 (MQ=255)
         gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                                                >  1:3131719/1‑70 (MQ=255)
         gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                               <  1:585195/71‑1 (MQ=255)
         gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                               <  1:3421319/71‑1 (MQ=255)
          aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                                              <  1:1714656/71‑1 (MQ=255)
            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                            <  1:4374755/71‑1 (MQ=255)
            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                            >  1:5113055/1‑71 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                          <  1:743625/69‑1 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                          <  1:5619305/69‑1 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                          <  1:4481914/69‑1 (MQ=255)
                 agatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcg                                                       <  1:4996120/68‑1 (MQ=255)
                   atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                                     >  1:2999489/2‑69 (MQ=255)
                   atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                                     >  1:5531915/2‑69 (MQ=255)
                   atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                                     >  1:4868777/2‑69 (MQ=255)
                    tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                                     >  1:1524639/1‑70 (MQ=255)
                    tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                                     >  1:5297841/1‑70 (MQ=255)
                    tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                                    <  1:3213693/71‑1 (MQ=255)
                      cAGTTCCGGTGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                                   <  1:2054571/70‑1 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                                             >  1:5344057/1‑60 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                                  <  1:4187216/71‑1 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                                  <  1:2744063/71‑1 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                                  >  1:730371/1‑71 (MQ=255)
                      cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                                   <  1:1520136/70‑1 (MQ=255)
                      cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                                   <  1:3098146/70‑1 (MQ=255)
                        gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                                >  1:2763614/1‑71 (MQ=255)
                        gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                                <  1:1366105/71‑1 (MQ=255)
                         ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                               <  1:3277419/55‑1 (MQ=255)
                         ctCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                                   <  1:2434266/66‑1 (MQ=255)
                          tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                                               >  1:1682187/1‑70 (MQ=255)
                          tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                                               >  1:3631966/1‑70 (MQ=255)
                            cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                                            >  1:37593/1‑71 (MQ=255)
                            cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgg                                                                        >  1:5159091/1‑43 (MQ=16)
                            cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcga                                                      <  1:4585500/61‑2 (MQ=255)
                              gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc                                                        <  1:2283399/57‑1 (MQ=255)
                                  gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                      <  1:1173550/71‑1 (MQ=255)
                                  gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                      <  1:1631914/71‑1 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                               <  1:1623944/45‑1 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                           <  1:886589/49‑1 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                           <  1:2549212/49‑1 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                                    >  1:2779726/1‑56 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                     >  1:2634568/1‑71 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                     >  1:4463565/1‑71 (MQ=255)
                                   aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                     >  1:3536593/1‑71 (MQ=255)
                                    tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                                 >  1:5393793/1‑42 (MQ=255)
                                    tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                                 >  1:4060995/1‑42 (MQ=255)
                                    tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                        <  1:3841223/67‑1 (MQ=255)
                                    tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg                                    <  1:182418/71‑1 (MQ=255)
                                      cGCAGGTACATGTCGAGATCCAGCGCGTTATTGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                   <  1:827530/70‑1 (MQ=255)
                                      cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                                       >  1:2929089/1‑66 (MQ=255)
                                      cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                   <  1:3943651/70‑1 (MQ=255)
                                       gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                                             <  1:211022/59‑1 (MQ=255)
                                        cAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCa                                <  1:2229378/71‑1 (MQ=255)
                                         aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc                               <  1:1902217/71‑1 (MQ=255)
                                          ggTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                                           <  1:167878/42‑1 (MQ=255)
                                           gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                             <  1:2411358/71‑1 (MQ=255)
                                            tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                                    <  1:4856365/47‑1 (MQ=255)
                                            tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTg                            >  1:3909127/1‑71 (MQ=255)
                                              cATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                                            <  1:196368/37‑1 (MQ=255)
                                               aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat                         >  1:3289528/1‑71 (MQ=255)
                                               aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGca                          >  1:5083439/1‑70 (MQ=255)
                                                tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                                     <  1:1338097/42‑1 (MQ=255)
                                                tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatc                        <  1:2953586/71‑1 (MQ=255)
                                                  tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                        <  1:2705047/53‑1 (MQ=255)
                                                  tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatca                       <  1:3846328/70‑1 (MQ=255)
                                                    gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg                    >  1:1910079/1‑71 (MQ=255)
                                                     agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                                              >  1:5679920/1‑44 (MQ=255)
                                                     agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                                              >  1:755035/1‑44 (MQ=255)
                                                       aTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc                  >  1:218407/1‑70 (MQ=255)
                                                         ccAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAcc                              >  1:4806698/1‑56 (MQ=255)
                                                           aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc             <  1:299525/71‑1 (MQ=255)
                                                            gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc             >  1:1235064/1‑70 (MQ=255)
                                                            gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCa            <  1:2873253/71‑1 (MQ=255)
                                                              gcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa           <  1:4238244/70‑1 (MQ=255)
                                                               cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt         >  1:3994413/1‑71 (MQ=255)
                                                                gTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACtt        >  1:4633813/1‑71 (MQ=255)
                                                                 ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc       >  1:1266663/1‑71 (MQ=255)
                                                                 ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc       >  1:4341644/1‑71 (MQ=255)
                                                                  tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc       >  1:2907183/1‑70 (MQ=255)
                                                                  tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc       >  1:2900545/1‑70 (MQ=255)
                                                                   atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa     >  1:1629431/1‑71 (MQ=255)
                                                                   atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa     >  1:5466831/1‑71 (MQ=255)
                                                                   aTGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa     >  1:2968749/1‑71 (MQ=255)
                                                                    tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa     <  1:768147/70‑1 (MQ=255)
                                                                    tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt    <  1:5063368/71‑1 (MQ=255)
                                                                      |                                                                      
CAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAA  >  Exported/1933695‑1933835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: