Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,795 G→A 14.2% G216G (GGC→GGT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,7950GA14.2% 173.1 / 15.0 70G216G (GGC→GGTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (24/36);  new base A (8/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 3.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATAC  >  Exported/1933726‑1933865
                                                                     |                                                                      
cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGCTAAACGGACGCGCAGCGGCACCGc                                                                       <  1:5365137/71‑1 (MQ=255)
 gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                                                      <  1:993792/71‑1 (MQ=255)
   gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                                                    <  1:1173550/71‑1 (MQ=255)
   gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                                                    <  1:1631914/71‑1 (MQ=255)
   gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                                                    <  1:1766010/71‑1 (MQ=255)
    aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                                                   >  1:3536593/1‑71 (MQ=255)
    aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                                                   >  1:4510568/1‑71 (MQ=255)
    aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                                                   >  1:4463565/1‑71 (MQ=255)
    aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                                                   >  1:2634568/1‑71 (MQ=255)
     tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                                                      <  1:3841223/67‑1 (MQ=255)
     tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg                                                                  <  1:182418/71‑1 (MQ=255)
       cGCAGGTACATGTCGAGATCCAGCGCGTTATTGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                                                 <  1:827530/70‑1 (MQ=255)
       cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                                                                     >  1:2929089/1‑66 (MQ=255)
       cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                                                 <  1:3943651/70‑1 (MQ=255)
         cAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCa                                                              <  1:2229378/71‑1 (MQ=255)
          aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc                                                             <  1:1902217/71‑1 (MQ=255)
            gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                                                           <  1:2411358/71‑1 (MQ=255)
             tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTg                                                          >  1:3909127/1‑71 (MQ=255)
                aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat                                                       >  1:3289528/1‑71 (MQ=255)
                aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGca                                                        >  1:5083439/1‑70 (MQ=255)
                 tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatc                                                      <  1:2953586/71‑1 (MQ=255)
                   tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                                                      <  1:2705047/53‑1 (MQ=255)
                   tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatca                                                     <  1:3846328/70‑1 (MQ=255)
                     gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                                                           >  1:2118789/1‑62 (MQ=255)
                     gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg                                                  >  1:1910079/1‑71 (MQ=255)
                        aTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc                                                >  1:218407/1‑70 (MQ=255)
                          ccAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAcc                                                            >  1:4806698/1‑56 (MQ=255)
                            aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc                                           <  1:299525/71‑1 (MQ=255)
                             gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc                                           >  1:1235064/1‑70 (MQ=255)
                             gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCa                                          <  1:2873253/71‑1 (MQ=255)
                               gcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa                                         <  1:4238244/70‑1 (MQ=255)
                                cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt                                       >  1:3994413/1‑71 (MQ=255)
                                cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt                                       <  1:4157284/71‑1 (MQ=255)
                                 gTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACtt                                      >  1:4633813/1‑71 (MQ=255)
                                  ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc                                     >  1:1266663/1‑71 (MQ=255)
                                  ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc                                     >  1:4341644/1‑71 (MQ=255)
                                   tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc                                     >  1:2907183/1‑70 (MQ=255)
                                   tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc                                     >  1:2900545/1‑70 (MQ=255)
                                    atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa                                   >  1:5466831/1‑71 (MQ=255)
                                    atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa                                   >  1:1629431/1‑71 (MQ=255)
                                    aTGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa                                   >  1:2968749/1‑71 (MQ=255)
                                     tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa                                   <  1:768147/70‑1 (MQ=255)
                                     tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt                                  <  1:5063368/71‑1 (MQ=255)
                                      gatgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTACCAt                                  >  1:5275157/3‑70 (MQ=255)
                                       gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATaa                                <  1:2920968/71‑1 (MQ=255)
                                          ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc                             <  1:2354082/71‑1 (MQ=255)
                                          ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc                             <  1:1764973/71‑1 (MQ=255)
                                          ggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAg                              <  1:1906686/70‑1 (MQ=255)
                                           gtaaTAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGcc                            <  1:4942852/68‑1 (MQ=255)
                                             gATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgc                          >  1:3084545/1‑71 (MQ=255)
                                              aTAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgcg                         >  1:1694715/1‑71 (MQ=255)
                                               tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg                        <  1:4098648/71‑1 (MQ=255)
                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATaaa                               >  1:810487/1‑63 (MQ=12)
                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgc                        >  1:980354/1‑69 (MQ=255)
                                                 aaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcgc                      >  1:2829337/1‑68 (MQ=255)
                                                    ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg                           <  1:1974073/63‑1 (MQ=255)
                                                    ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc                   <  1:3778846/71‑1 (MQ=255)
                                                     gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc                   >  1:5549767/1‑70 (MQ=255)
                                                        gcgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATg               <  1:832179/71‑1 (MQ=255)
                                                         cgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt                                            <  1:1535552/41‑1 (MQ=255)
                                                            aGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt           <  1:910343/71‑1 (MQ=255)
                                                                gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg       <  1:1211218/71‑1 (MQ=255)
                                                                gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg       <  1:4079909/71‑1 (MQ=255)
                                                                gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg       <  1:5306629/71‑1 (MQ=255)
                                                                gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg       <  1:1625086/71‑1 (MQ=255)
                                                                  aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCAt                <  1:4141221/60‑1 (MQ=255)
                                                                  aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa     >  1:3102022/1‑71 (MQ=255)
                                                                  aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa     >  1:4014552/1‑71 (MQ=255)
                                                                  aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa     >  1:4413358/1‑71 (MQ=255)
                                                                   ccGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCAGCGGTTCACCATg               <  1:4670574/60‑1 (MQ=255)
                                                                     gCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATAc  >  1:5546473/1‑71 (MQ=255)
                                                                     |                                                                      
CGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATAC  >  Exported/1933726‑1933865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: