Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,819 C→G 16.2% T208T (ACG→ACC lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,8190CG16.2% 161.0 / 21.5 68T208T (ACG→ACClysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (24/33);  new base G (9/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

ATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTT  >  Exported/1933750‑1933889
                                                                     |                                                                      
aTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc                                                                        >  1:218407/1‑70 (MQ=255)
    aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc                                                                   <  1:299525/71‑1 (MQ=255)
     gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc                                                                   >  1:1235064/1‑70 (MQ=255)
     gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCa                                                                  <  1:2873253/71‑1 (MQ=255)
       gcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa                                                                 <  1:4238244/70‑1 (MQ=255)
        cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt                                                               <  1:4157284/71‑1 (MQ=255)
        cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt                                                               >  1:3994413/1‑71 (MQ=255)
         gTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACtt                                                              >  1:4633813/1‑71 (MQ=255)
          ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc                                                             >  1:4341644/1‑71 (MQ=255)
          ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc                                                             >  1:1266663/1‑71 (MQ=255)
           tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc                                                             >  1:2900545/1‑70 (MQ=255)
           tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc                                                             >  1:2907183/1‑70 (MQ=255)
            atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa                                                           >  1:1629431/1‑71 (MQ=255)
            atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa                                                           >  1:5466831/1‑71 (MQ=255)
            aTGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa                                                           >  1:2968749/1‑71 (MQ=255)
             tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa                                                           <  1:768147/70‑1 (MQ=255)
             tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt                                                          <  1:5063368/71‑1 (MQ=255)
              gatgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTACCAt                                                          >  1:5275157/3‑70 (MQ=255)
               gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATaa                                                        <  1:2920968/71‑1 (MQ=255)
                  ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc                                                     <  1:1764973/71‑1 (MQ=255)
                  ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc                                                     <  1:2354082/71‑1 (MQ=255)
                  ggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAg                                                      <  1:1906686/70‑1 (MQ=255)
                   gtaaTAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGcc                                                    <  1:4942852/68‑1 (MQ=255)
                     gATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgc                                                  >  1:3084545/1‑71 (MQ=255)
                      aTAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgcg                                                 >  1:1694715/1‑71 (MQ=255)
                       tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg                                                <  1:4098648/71‑1 (MQ=255)
                        aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATaaa                                                       >  1:810487/1‑63 (MQ=12)
                        aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgc                                                >  1:980354/1‑69 (MQ=255)
                         aaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcgc                                              >  1:2829337/1‑68 (MQ=255)
                            ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg                                                   <  1:1974073/63‑1 (MQ=255)
                            ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc                                           <  1:3778846/71‑1 (MQ=255)
                             gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc                                           >  1:5549767/1‑70 (MQ=255)
                                gcgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATg                                       <  1:832179/71‑1 (MQ=255)
                                 cgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt                                                                    <  1:1535552/41‑1 (MQ=255)
                                    aGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt                                   <  1:910343/71‑1 (MQ=255)
                                        gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:5306629/71‑1 (MQ=255)
                                        gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:4079909/71‑1 (MQ=255)
                                        gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:1211218/71‑1 (MQ=255)
                                        gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:1625086/71‑1 (MQ=255)
                                          aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCAt                                        <  1:4141221/60‑1 (MQ=255)
                                          aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa                             >  1:4014552/1‑71 (MQ=255)
                                          aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa                             >  1:4413358/1‑71 (MQ=255)
                                          aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa                             >  1:3102022/1‑71 (MQ=255)
                                           ccGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCAGCGGTTCACCATg                                       <  1:4670574/60‑1 (MQ=255)
                                             gCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATAc                          >  1:5546473/1‑71 (MQ=255)
                                               cAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCa                        <  1:1764683/71‑1 (MQ=255)
                                                 gggATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCaga                      <  1:452627/71‑1 (MQ=255)
                                                  ggATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:4245741/61‑1 (MQ=255)
                                                   gATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCa                                            >  1:369778/1‑47 (MQ=255)
                                                   gATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCaga                      >  1:3739751/1‑69 (MQ=255)
                                                    aTCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgcg                                                 >  1:1619219/1‑41 (MQ=255)
                                                     tCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCagagag                   >  1:5295375/1‑70 (MQ=255)
                                                       aCCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACgga                             >  1:5677450/1‑56 (MQ=16)
                                                         cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGa                                      <  1:5352279/47‑1 (MQ=255)
                                                         cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                               <  1:2535973/54‑1 (MQ=255)
                                                         cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCt              <  1:810334/71‑1 (MQ=255)
                                                          tGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGAt                >  1:1117647/1‑68 (MQ=255)
                                                           gCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAAt                            <  1:2922749/55‑1 (MQ=255)
                                                           gCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCagaga                    >  1:4572828/1‑63 (MQ=255)
                                                           gCATCATCGGCGTTTCAACTTCCATAAAGCCGCCGTTCAcc                                          >  1:944456/1‑41 (MQ=255)
                                                            catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc            <  1:5341534/70‑1 (MQ=255)
                                                            catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc            <  1:2209908/70‑1 (MQ=255)
                                                            catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc            <  1:4214645/70‑1 (MQ=255)
                                                                atcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGc                                >  1:5338288/1‑46 (MQ=255)
                                                                atcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg       <  1:2856231/71‑1 (MQ=255)
                                                                 tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa                              >  1:2591118/1‑47 (MQ=255)
                                                                 tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa                              >  1:4252413/1‑47 (MQ=255)
                                                                 tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg       >  1:4508284/1‑70 (MQ=255)
                                                                  cGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt                                   <  1:3181212/41‑1 (MQ=255)
                                                                     cGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACtt  <  1:5378127/71‑1 (MQ=255)
                                                                     |                                                                      
ATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTT  >  Exported/1933750‑1933889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: