Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,934,253 G→A 17.3% L64L (CTG→TTG) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,934,2530GA17.3% 232.9 / 36.1 104L64L (CTG→TTG) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (39/47);  new base A (11/7);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 105 total aligned reads displayed.

AACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAC  >  Exported/1934185‑1934321
                                                                    |                                                                    
aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg                                                                    >  1:4978297/1‑71 (MQ=255)
   gAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt                                                                  >  1:2343918/1‑70 (MQ=255)
    aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                                                >  1:3316697/1‑71 (MQ=255)
     aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc                                                               <  1:2878285/71‑1 (MQ=255)
        aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt                                                            <  1:4023622/69‑1 (MQ=255)
         ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt                                                           >  1:4066856/2‑71 (MQ=255)
          gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                                          >  1:2613270/1‑71 (MQ=255)
            tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct                                                        >  1:3144320/1‑69 (MQ=255)
              tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc                                                       <  1:2759822/70‑1 (MQ=255)
               aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                                      <  1:776813/70‑1 (MQ=255)
                cccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                                    <  1:4873818/71‑1 (MQ=255)
                  cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCtt                                                                 >  1:4280273/1‑56 (MQ=255)
                  cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct                                                  <  1:321230/71‑1 (MQ=255)
                    taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                                                <  1:2739660/55‑1 (MQ=255)
                    taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                                                <  1:2634463/55‑1 (MQ=255)
                    taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                                                <  1:2608632/55‑1 (MQ=255)
                    taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                                    <  1:1588650/67‑1 (MQ=255)
                    taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct                                                >  1:2161738/1‑71 (MQ=255)
                     aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt                                               >  1:386593/1‑71 (MQ=255)
                     aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt                                               >  1:949985/1‑71 (MQ=255)
                     aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt                                               >  1:740975/1‑71 (MQ=255)
                      ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                                              >  1:435370/1‑71 (MQ=255)
                      ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                                              <  1:846781/71‑1 (MQ=255)
                      ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                                              >  1:3534875/1‑71 (MQ=255)
                      ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                                              >  1:5287180/1‑71 (MQ=255)
                      ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                                              >  1:2285625/1‑71 (MQ=255)
                       taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc                                             >  1:5462633/1‑70 (MQ=255)
                       taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc                                             >  1:666016/1‑70 (MQ=255)
                         cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCa                                                              <  1:5205759/52‑1 (MQ=255)
                         cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc                                           <  1:2983383/71‑1 (MQ=255)
                         cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc                                            <  1:4796101/70‑1 (MQ=255)
                         cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc                                            <  1:3307086/70‑1 (MQ=255)
                             gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg                                       <  1:3866528/71‑1 (MQ=255)
                               gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa                                     <  1:1442612/71‑1 (MQ=255)
                                 catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt                                                        <  1:1445814/50‑1 (MQ=255)
                                 catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                                    >  1:618336/1‑70 (MQ=255)
                                  atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt                                                            <  1:4439579/45‑1 (MQ=255)
                                  atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                                              >  1:4256860/1‑59 (MQ=255)
                                  atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                                              >  1:555288/1‑59 (MQ=255)
                                  atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt                                   <  1:686803/70‑1 (MQ=255)
                                   tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                                                <  1:2284141/40‑1 (MQ=255)
                                   tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc                                        <  1:3931232/64‑1 (MQ=255)
                                   tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt                                 <  1:4701572/71‑1 (MQ=255)
                                   taatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                                              >  1:5677460/3‑58 (MQ=255)
                                    catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc                                                         <  1:4904945/46‑2 (MQ=255)
                                    catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc                                                         <  1:4911708/46‑2 (MQ=255)
                                    catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                                          >  1:1633243/1‑45 (MQ=255)
                                    catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                                          >  1:4980481/1‑45 (MQ=255)
                                    catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                                          >  1:4986892/1‑45 (MQ=255)
                                    catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa                                      <  1:4416385/65‑1 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                                      >  1:5287455/1‑48 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                                    >  1:5369954/1‑50 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg                                <  1:923928/70‑1 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg                                <  1:4031458/70‑1 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc                               >  1:2425460/1‑71 (MQ=255)
                                     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCCTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg                                <  1:4652666/70‑1 (MQ=255)
                                     atGAGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                                      >  1:4521076/1‑48 (MQ=255)
                                       gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                             >  1:4291881/1‑71 (MQ=255)
                                       gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                             >  1:17456/1‑71 (MQ=255)
                                         ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg                            <  1:1099486/70‑1 (MQ=255)
                                         ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGc                           <  1:3994730/71‑1 (MQ=255)
                                           ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg                                             <  1:5267452/51‑1 (MQ=255)
                                           ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa                         >  1:48892/1‑71 (MQ=255)
                                              gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                      >  1:714320/1‑71 (MQ=255)
                                               cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa                                     >  1:3238388/1‑55 (MQ=255)
                                                aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:2595652/71‑1 (MQ=255)
                                                aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:807830/71‑1 (MQ=255)
                                                aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    >  1:3061736/1‑71 (MQ=255)
                                                 aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:3417545/70‑1 (MQ=255)
                                                 aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTATGCGTGCAATTGGt                    >  1:1375951/1‑70 (MQ=255)
                                                   ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc                                                 <  1:2081012/39‑1 (MQ=255)
                                                   ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc                                                 <  1:5345666/39‑1 (MQ=255)
                                                   ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa                         >  1:1073703/1‑63 (MQ=255)
                                                   ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa                         >  1:3461357/1‑63 (MQ=255)
                                                   ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa                         >  1:700856/1‑63 (MQ=255)
                                                    gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:4214467/67‑1 (MQ=255)
                                                    gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa                  >  1:851014/1‑69 (MQ=255)
                                                    gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTATTTGCCGTCGAATTCTGCGCGCAATTGGTCa                  >  1:1746159/1‑69 (MQ=255)
                                                      gACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg              >  1:4700983/1‑71 (MQ=255)
                                                       aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag                 <  1:2590850/67‑1 (MQ=255)
                                                       aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag                 <  1:5408656/67‑1 (MQ=255)
                                                       aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag                 <  1:2462445/67‑1 (MQ=255)
                                                        cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTa            >  1:2552620/1‑71 (MQ=255)
                                                        cTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTa            <  1:3182353/71‑1 (MQ=255)
                                                         ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa                                      <  1:5508224/44‑1 (MQ=255)
                                                         ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt                                 <  1:1077842/49‑1 (MQ=255)
                                                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt           <  1:3968678/69‑1 (MQ=255)
                                                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATg          >  1:3166334/1‑70 (MQ=255)
                                                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa         >  1:2747049/1‑71 (MQ=255)
                                                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa         >  1:874971/1‑71 (MQ=255)
                                                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa         >  1:3159777/1‑71 (MQ=255)
                                                            gATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa         <  1:2304240/70‑1 (MQ=255)
                                                             aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:4991798/58‑1 (MQ=255)
                                                             aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATc       <  1:813340/71‑1 (MQ=255)
                                                                ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                    <  1:1739618/55‑1 (MQ=255)
                                                                ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc     <  1:3862576/70‑1 (MQ=255)
                                                                  cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAGTTCTGCGTGCAATTGGt                    >  1:759464/1‑53 (MQ=255)
                                                                  cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc  >  1:5045988/1‑71 (MQ=255)
                                                                   aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt                                 <  1:3900552/39‑1 (MQ=255)
                                                                   aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc  <  1:4588395/70‑1 (MQ=255)
                                                                    |                                                                    
AACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAC  >  Exported/1934185‑1934321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: