| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,002,416 | A→G | 11.7% | intergenic (+63/+60) | yghU → / ← hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,002,416 | 0 | A | G | 11.7% | 180.9 / 12.4 | 68 | intergenic (+63/+60) | yghU/hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (36/24); new base G (3/5); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
AGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGG > Exported/2002346‑2002486 | aGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa > 1:628797/1‑71 (MQ=255)aGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa < 1:5217679/71‑1 (MQ=255) gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a > 1:990705/1‑71 (MQ=255) gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a < 1:4667310/71‑1 (MQ=255) gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGc < 1:914092/70‑1 (MQ=255) gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGc < 1:3137013/70‑1 (MQ=255) tgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑Ataga > 1:4184107/1‑67 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTg < 1:1579360/70‑1 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGa < 1:3091002/71‑1 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGa < 1:2235435/71‑1 (MQ=255) tcgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCATGCCGCATCCGGCAATCGTGCACA‑ATGCCTGAt > 1:2414298/1‑71 (MQ=255) tcgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGAt > 1:3347479/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:3395797/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:3300464/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:800646/1‑71 (MQ=255) gtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGc < 1:2251105/71‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc < 1:3554549/71‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:3892702/1‑71 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:4786456/1‑71 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:2601440/1‑71 (MQ=255) gcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑t > 1:2718058/1‑71 (MQ=255) gcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑t > 1:1706087/1‑71 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg > 1:549561/1‑71 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg > 1:3460609/1‑71 (MQ=255) aGCCATCCAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGcc > 1:300041/1‑71 (MQ=255) aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGc > 1:5213317/1‑70 (MQ=255) cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCcgcg > 1:3412365/1‑71 (MQ=255) taGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCt > 1:1747449/2‑71 (MQ=11) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCt > 1:547717/1‑70 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTa < 1:2424158/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCtt < 1:2369869/70‑1 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTa > 1:5575257/1‑71 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:398235/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:1976584/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTAt < 1:366548/71‑1 (MQ=1) ccGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCa < 1:2557997/71‑1 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgg > 1:103501/1‑71 (MQ=1) gATAAGGCGTTCACGCCGCATCCGGCAATCGTGcac < 1:1027287/36‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgac < 1:2140569/71‑3 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGcc > 1:299414/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGc > 1:5086911/1‑50 (MQ=255) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTa > 1:502717/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCt < 1:143691/70‑1 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCt < 1:4704908/39‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGGCCTAc < 1:4056090/71‑1 (MQ=1) ggCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGc < 1:4087008/43‑1 (MQ=255) gCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa < 1:5425052/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:2040050/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4972006/71‑1 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2818161/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1811793/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2566937/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:5073097/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:445070/1‑71 (MQ=255) cACGCCGCATCCGGCAATCGTTCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc < 1:2863666/70‑1 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa > 1:448828/1‑66 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1488499/1‑70 (MQ=255) aCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCt < 1:270106/70‑1 (MQ=255) aCGCCGCATCCGGCAATCGAGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTa < 1:3990030/71‑1 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:3214259/1‑69 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc < 1:5619053/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1283253/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1862813/71‑1 (MQ=255) ccGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcg > 1:853808/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:1066588/1‑62 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:3899269/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:2264226/1‑71 (MQ=255) gCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc > 1:313665/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:3221371/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:4051214/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4997144/52‑1 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:4606671/1‑71 (MQ=255) aTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg < 1:1021144/71‑1 (MQ=255) | AGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGG > Exported/2002346‑2002486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |